Polyacrylamide Nanoparticles with Visible and Near-Infrared Autofluorescence

Xie, Hongmei; Zhang, Ling; Wu, Lin; Wang, Jinke

doi:10.1002/ppsc.201700222

Cited by 6 publications

(5 citation statements)

References 48 publications

(45 reference statements)

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“…In addition, due to the use of APMA and ε-PL, PAMNPs had positive charges. Our study also demonstrated that PAMNPs had high biocompatibility in vitro and in vivo …”

Section: Introductionsupporting

confidence: 72%

“…The surface properties of polyacrylamide nanoparticles (PAMNPs) can be modified through introducing functional reactive groups by adding different monomers during the preparation process such as introducing the carboxyl groups by adding acrylic acid monomer and introducing the amino groups by adding APMA . We have previously synthesized a kind of PAMNP with autofluorescence of both visible fluorescence (VF) and near-infrared fluorescence (NIRF) . This kind of autofluorescence is generated by glutaraldehyde (GTA) cross-linking, which produces two different double bonds, CN and CC .…”

Section: Introductionmentioning

confidence: 99%

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Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection

Wang

Zhang

et al. 2020

ACS Omega

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The fast and cost-effective DNA extraction is critical for all DNA-based detections. Here, we fabricated a new kind of polyacrylamide microsphere (PAMMP) in various sizes with two methods, spot polymerization (large size but low yield) and modified inverse microemulsion polymerization (small size but high yield). The fabricated PAMMPs have strong autofluorescence (fPAMMPs), including both visible fluorescence (VF) and near-infrared fluorescence (NIRF), which can remain very stable in various stringent conditions including strong acid and alkali and high temperature. The fabricated fPAMMPs were also highly positively charged, which could be used to effectively capture various biomolecules such as IRDye 800-labeled streptavidin and DNA. We thus developed a new method for rapid extraction (3−5 min) of DNA from various samples including bacteria, mammalian cells, plant and animal solid tissues, and human blood plasma using fPAMMPs. Moreover, the DNA captured on fPAMMPs (fPAMMP@DNA) could be effectively detected by both normal and quantitative PCR amplifications. Finally, we showed that NaBH 4 treatment removed autofluorescence in fPAMMPs (PAMMPs), which could also be applied to DNA extraction and PCR detection. In conclusion, we here fabricated new kinds of fPAMMPs and PAMMPs, developed a new rapid DNA extraction method, and demonstrated their useful applications in PCR detection.

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“…In addition, due to the use of APMA and ε-PL, PAMNPs had positive charges. Our study also demonstrated that PAMNPs had high biocompatibility in vitro and in vivo …”

Section: Introductionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection

Wang

Zhang

et al. 2020

ACS Omega

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“…Therefore, polyacrylamide, a hydrophilic polymer, was selected because of its biocompatibility and low toxicity . Polyacrylamide nanobeads have been used for a wide array of biomedical applications ranging from imaging and MRI contrast to photodynamic therapy because they are amenable to the addition of fluorophores, photosensitizers, or to encapsulate magnetic iron oxide nanoparticles …”

Section: Resultsmentioning

confidence: 99%

Paramagnetic Clusters of Mn₃(O₂CCH₃)₆(Bpy)₂ in Polyacrylamide Nanobeads as a New Design Approach to a T₁–T₂ Multimodal Magnetic Resonance Imaging Contrast Agent

Dahanayake¹,

Pornrungroj

Pablico-Lansigan

et al. 2019

ACS Appl. Mater. Interfaces

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There is an increasing need for gadolinium-free magnetic resonance imaging (MRI) contrast agents, particularly for patients suffering from chronic kidney disease. Using a cluster−nanocarrier combination, we have identified a novel approach to the design of biomedical nanomaterials and report here the criteria for the cluster and the nanocarrier and the advantages of this combination. We have investigated the relaxivity of the following manganese oxo clusters: the parent cluster Mn 3 (O 2 CCH 3 ) 6 (Bpy) 2 (1) where Bpy = 2,2′-bipyridine and three new analogs, Mn 3 (O 2 CC 6 H 4 CHCH 2 ) 6 (Bpy) 2 (2), Mn 3 (O 2 CC(CH 3 )CH 2 ) 6 (Bpy) 2 (3), and Mn 3 O(O 2 CCH 3 ) 6 (Pyr) 2 (4) where Pyr = pyridine. The parent cluster, Mn 3 (O 2 CCH 3 ) 6 (Bpy) 2 (1), had impressive relaxivity (r 1 = 6.9 mM −1 s −1 , r 2 = 125 mM −1 s −1 ) and was found to be the most amenable for the synthesis of cluster-nanocarrier nanobeads. Using the inverse miniemulsion polymerization technique (1) in combination with the hydrophilic monomer acrylamide, we synthesized nanobeads (∼125 nm diameter) with homogeneously dispersed clusters within the polyacrylamide matrix (termed Mn 3 Bpy-PAm). The nanobeads were surfacemodified by co-polymerization with an amine-functionalized monomer. This enabled various postsynthetic modifications, for example, to attach a near-IR dye, Cyanine7, as well as a targeting agent. When evaluated as a potential multimodal MRI contrast agent, high relaxivity and contrast were observed with r 1 = 54.4 mM −1 s −1 and r 2 = 144 mM −1 s −1 , surpassing T 1 relaxivity of clinically used Gd-DTPA chelates as well as comparable T 2 relaxivity to iron oxide microspheres. Physicochemical properties, cellular uptake, and impacts on cell viability were also investigated.

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“…In functional analyses of interactions between microbes such as endophytes and roots, the labeling of bacteria with fluorescent proteins or application of transcriptional fusions with reporter genes to visualize and measure bacterial gene expression is very common (Buschart et al ., 2012; Chen et al ., 2015; Sarkar et al ., 2017). For fluorescent staining, the signal may be enhanced by increasing the amount of the fluorescent dye and eliminating the autofluorescence by irradiation with light before staining (Neumann & Gabel, 2002), or by treating the sample with some autofluorescence reducing agent (Xie et al ., 2017). However, for in vivo studies applying fluorescent proteins, the autofluorescence is problematic, especially when the fluorescent protein is not strongly expressed.…”

Section: Introductionmentioning

confidence: 99%

Deep‐learning‐based removal of autofluorescence and fluorescence quantification in plant‐colonizing bacteria in vivo

2022

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Summary Fluorescence microscopy is common in bacteria–plant interaction studies. However, strong autofluorescence from plant tissues impedes in vivo studies on endophytes tagged with fluorescent proteins. To solve this problem, we developed a deep‐learning‐based approach to eliminate plant autofluorescence from fluorescence microscopy images, tested for the model endophyte Azoarcus olearius BH72 colonizing Oryza sativa roots. Micrographs from three channels (tdTomato for gene expression, green fluorescent protein (GFP) and AutoFluorescence (AF)) were processed by a neural network based approach, generating images that simulate the background autofluorescence in the tdTomato channel. After subtracting the model‐generated signals from each pixel in the genuine channel, the autofluorescence in the tdTomato channel was greatly reduced or even removed. The deep‐learning‐based approach can be applied for fluorescence detection and quantification, exemplified by a weakly expressed, a cell‐density modulated and a nitrogen‐fixation gene in A. olearius. A transcriptional nifH::tdTomato fusion demonstrated stronger induction of nif genes inside roots than outside, suggesting extension of the rhizosphere effect for diazotrophs into the endorhizosphere. The pre‐trained convolutional neural network model is easily applied to process other images of the same plant tissues with the same settings. This study showed the high potential of deep‐learning‐based approaches in image processing. With proper training data and strategies, autofluorescence in other tissues or materials can be removed for broad applications.

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Polyacrylamide Nanoparticles with Visible and Near-Infrared Autofluorescence

Cited by 6 publications

References 48 publications

Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection

Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection

Paramagnetic Clusters of Mn₃(O₂CCH₃)₆(Bpy)₂ in Polyacrylamide Nanobeads as a New Design Approach to a T₁–T₂ Multimodal Magnetic Resonance Imaging Contrast Agent

Deep‐learning‐based removal of autofluorescence and fluorescence quantification in plant‐colonizing bacteria in vivo

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Polyacrylamide Nanoparticles with Visible and Near-Infrared Autofluorescence

Cited by 6 publications

References 48 publications

Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection

Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection

Paramagnetic Clusters of Mn3(O2CCH3)6(Bpy)2 in Polyacrylamide Nanobeads as a New Design Approach to a T1–T2 Multimodal Magnetic Resonance Imaging Contrast Agent

Deep‐learning‐based removal of autofluorescence and fluorescence quantification in plant‐colonizing bacteria in vivo

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Paramagnetic Clusters of Mn₃(O₂CCH₃)₆(Bpy)₂ in Polyacrylamide Nanobeads as a New Design Approach to a T₁–T₂ Multimodal Magnetic Resonance Imaging Contrast Agent