Polyamine Analogues of Propanediamine Series Inhibit Prostate Tumor Cell Growth and Activate the Polyamine Catabolic Pathway

Плосконос, М. В.; Syatkin, S. P.; Неборак, Е. В.; Hilal, Abdullah; Sungrapova, Kristina Y.; Sokuyev, Roman I.; Благонравов, М. Л.; Korshunova, A Yu; Terentyev, A. A.

doi:10.21873/anticanres.14085

Cited by 7 publications

(7 citation statements)

References 14 publications

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“…The products of PA catabolism (mainly reactive oxygen species (ROS), such as hydrogen peroxide and acrolein) are capable of inducing cell death via apoptosis [25,26,32,33]. Apoptosis was the type of cell death in HepG2 cells when 4e was coincubated with Spd/Spm.…”

Section: Discussionmentioning

confidence: 99%

“…The inhibition of these enzymes resulted in tumor sensitization to anticancer therapy [17,18] or in a decrease in tumor aggressiveness [19]. The degradation pathway of PAs includes different di-and polyamine oxidases [2,3] that are often reduced in cancer tissues [20,21], while the catabolic products of PA oxidation are able to induce apoptosis in normal and tumor cells [22][23][24][25]. Thus, activation of the PA catabolic pathway, primarily through a direct interaction with PA oxidases, may be a promising strategy for cancer therapy [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Anticancer Cytotoxic Activity of Bispidine Derivatives Associated with the Increasing Catabolism of Polyamines

et al. 2022

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Polyamine (PA) catabolism is often reduced in cancer cells. The activation of this metabolic pathway produces cytotoxic substances that might cause apoptosis in cancer cells. Chemical compounds able to restore the level of PA catabolism in tumors could become potential antineoplastic agents. The search for activators of PA catabolism among bicyclononan-9-ones is a promising strategy for drug development. The aim of the study was to evaluate the biological activity of new 3,7-diazabicyclo[3.3.1]nonan-9-one derivatives that have antiproliferative properties by accelerating PA catabolism. Eight bispidine derivatives were synthetized and demonstrated the ability to activate PA catabolism in regenerating rat liver homogenates. However, only three of them demonstrated a potent ability to decrease the viability of cancer cells in the MTT assay. Compounds 4c and 4e could induce apoptosis more effectively in cancer HepG2 cells rather than in normal WI-38 fibroblasts. The lead compound 4e could significantly enhance cancer cell death, but not the death of normal cells if PAs were added to the cell culture media. Thus, the bispidine derivative 4e 3-(3-methoxypropyl)-7-[3-(1H-piperazin-1-yl)ethyl]-3,7-diazabicyclo[3.3.1]nonane could become a potential anticancer drug substance whose mechanism relies on the induction of PA catabolism in cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anticancer Cytotoxic Activity of Bispidine Derivatives Associated with the Increasing Catabolism of Polyamines

et al. 2022

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“…Each lobe has an iron-binding centre. The tertiary structures of apo-lactoferrin (iron-free lactoferrin) and iron-binding lactoferrin differ [4,6,7,8,9,10,13]. For example, it is found that Fе 3+ binding plays an essential role in implementing the antioxidant and bacteriostatic function of lactoferrin.…”

Section: R E L E V a N C Ementioning

confidence: 99%

Isolation and Purification of Protein That Is Immunologically Similar to Human Lactoferrin

Boiko¹,

Nikolaev²,

Kozak³

et al. 2021

ArveMED

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The study aimed at isolating a substance that is immunologically similar to human lactoferrin, hereinafter — microbial-derived lactoferrin (MdLF) isolated from K.pneumoniae liquid culture. Protein extraction started with isolation of ballast proteins in 2M ammonium sulphate (p.a.). Ion chromatography with cation-exchange agents was the basic method used for isolating MLF. Proteins from the isolated protein fractions were identified with AGID method. The isolated MLF is glucoprotein with the molecular mass of about 84,000, the agarose diffusion coefficient of 3.1 × 10–7 cm2 sec–1 and the relative electrophoretic mobility of 0.42. MLF is characterized by low hydrophobicity and elutes from phenylSepharose with 0.4 ammonium sulphate. Its isoelectric point equals to 9.2.

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“…1). They are essential for cell growth, cell proliferation and differentiation (8,9), and have the ability to coordinate the process of cell apoptosis (9)(10)(11)(12)(13). The contents of the PAs spermine (Spm) and spermidine (Spd) in the seminal plasma are significantly higher than in any other biological fluid, which highlights the importance of these low molecular weight organic polycations for human reproductive function, especially in men (10,14).…”

Section: Introductionmentioning

confidence: 99%

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Плосконос

Zulbalaeva²,

Kurbangalieva³

et al. 2022

Biomed Rep

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The aim of the present study was to investigate the influence of millimeter-wave electromagnetic (MW) irradiation on normal and pathological human sperm in vitro, and to evaluate a possible role of polyamines (PA) in this process. The stability of sperm membranes, the number of apoptotic gametes, and the content of seminal plasma PA in the ejaculates of fertile and subfertile men were compared before and after short-term MW electromagnetic exposure in vitro. The ejaculate samples were collected from healthy donors [n=25, age 22-38 years old (y.o.), average age 30.6±1.1 y.o. (mean ± SEM)] and from subfertile men (n=78, age 25-48 y.o., average age 34.1±0.8 y.o.) and exposed to MW radiation. The electromagnetic field had a wavelength of 7.1 mm, a frequency of 42.194 GHz and an exposure time of 20 min. The fragility of sperm membranes was evaluated by their resistance to sodium chloride solution (Milovanov test) and to acetic acid (Joel test). Acrosin activity was assayed spectrophotometrically. Apoptosis was determined by the externalization of phosphatidylserine on the outer side of the sperm membrane and propidium iodide staining. The PA levels were determined by agar gel electrophoretic fractionation. An increase in the resistance of sperm membranes, a decrease in acrosin activity, a decrease in the number of apoptotic gametes and a decrease in the seminal plasma PA concentrations were found after exposure of the native human sperm to low-intensity MW irradiation. Two types of reactions were revealed for the subfertile samples. The results revealed positive bio-effects of specific microwaves on the human semen and the participation of PA in the realization of these effects.

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Polyamine Analogues of Propanediamine Series Inhibit Prostate Tumor Cell Growth and Activate the Polyamine Catabolic Pathway

Cited by 7 publications

References 14 publications

Anticancer Cytotoxic Activity of Bispidine Derivatives Associated with the Increasing Catabolism of Polyamines

Anticancer Cytotoxic Activity of Bispidine Derivatives Associated with the Increasing Catabolism of Polyamines

Isolation and Purification of Protein That Is Immunologically Similar to Human Lactoferrin

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

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