Polyamines and the Cell Cycle of <i>Catharanthus roseus</i> Cells in Culture

Maki, Hisae; Ando, Satoshi; Kodama, Hiroaki; Komamine, Atsushi

doi:10.1104/pp.96.4.1008

Cited by 33 publications

(16 citation statements)

References 35 publications

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“…Developmental processes in plants that are, in part, controlled by cytokinin, may also be affected by PAs, and the promotive effect of cytokinins on endogenous PAs levels, especially on free putrescine, is pronounced (Cho 1983, Hemanataranjan and Vaishampayan 1984, Walker et al 1988. Putrescine is the most abundant PA in cycling cells (Maki et al 1991, Pfosser 1993, Pfosser et al 1990). The precise part a lowering of endogenous PA levels may play in the response to cytokinin starvation has yet to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Mader

Hanke

1996

J Plant Growth Regul

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An immunocytochemical method was used to determine the proportion of cells in the DNA synthesis (S phase) of the mitotic cycle in suspension cultures of soybean (Glycine max (L.) Merr. cv. Acme) callus of cotyledonary origin, the stably cytokinin-dependent tissue used in the cytokinin bioassay devised by Carlos O.Miller. A standard cell synchronization protocol involving hydroxyurea was used to demonstrate the applicability of the immunocytochemical method to this cell culture. Cells were brought to mitotic arrest by cytokinin withdrawal, and the cell division cycle was restarted by the addition of cytokinin. We have followed the pattern of resumption of S phase after the readdition of cytokinin. This pattern reveals the existence of three subpopulations of cells in cytokinin-starved cultures, consistent with the occurrence of three cytokinin-requiring events in the cell cycle: one in mitosis, one in S phase, and one in the G~ phase.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Mader

Hanke

1996

J Plant Growth Regul

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“…2-4), because of the high activity of ADC (Fig. 5) that is a prerequisite for cell division (Fracassini et al, 1980;Maki et al, 1991) leading to callus formation. High levels of free PAs (PUT) and ADC activity have been reported in growing and dividing tissues (Kaur-Sawhney et al, 1985.…”

Section: Discussionmentioning

confidence: 99%

Temporal Regulation of Somatic Embryogenesis by Adjusting Cellular Polyamine Content in Eggplant1

Yadav

Rajam

1998

Plant Physiology

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Four critical stages of embryogenesis, including callus induction, cellular acquisition of morphogenetic competence, expression of embryogenic program, and development and maturation of somatic embryos during somatic embryogenesis from leaf discs of eggplant (Solanum melongena L.), were identified by scanning electron microscopy. Temporal changes in arginine decarboxylase (ADC) activity and polyamines (PAs) during critical stages of embryogenesis revealed that high levels of PAs (especially putrescine [PUT]), due to higher ADC activity in discs from the apical region (with high embryogenic capacity) than from the basal region of the leaf (with poor embryogenic capacity), were correlated with differential embryogenesis response. Kinetic studies of the up-and downregulation of embryogenesis revealed that PUT and difluoromethylarginine pretreatments were most effective before the onset of embryogenesis. Basal discs pretreated with PUT for 4 to 7 d showed improved embryogenesis that was comparable to apical discs. PA content at various critical steps in embryogenesis from basal discs were found to be comparable to that of apical discs following adjustments of cellular PA content by PUT. In contrast, pretreatment of apical discs with difluoromethylarginine for 3 d significantly reduced ADC activity, cellular PA content, and embryogenesis to levels that were comparable to basal discs. Discs from the basal region of leaves treated with PUT for 3 d during the identified stages of embryogenesis improved their embryogenic potential.

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“…Putrescine may play an important role in the progression of the cell cycle and more especially in the transition from G1 to S and from the G 2 to M. phase (Maki et al 1991). An increased level of putrescine might inhibit DNA-metylation, permitting the expression of specific genes (Martin-Tanguy et al 1996).…”

Section: Discussionmentioning

confidence: 98%

Role of polyamines in cytokinin-dependent physiological processes. III. Changes in polyamine levels during cytokinin-induced formation of gametophore buds in Ceratodon purpureus

Legocka

Żarnowska

2002

Acta Physiol Plant

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Key words: Ceratodon purpureus, chromatin, g a m e t o p h o r e buds, kinetin, polyamines, ribosomes, inhibitors of polyamine biosynthesis PAs-polyamines, PCA-perchloric acid, Put-putrescine, S A M D C -S -a d e n o s y l m e t h i o n i n e decarboxylase, Spd-spermidine, Spm-spermine AbstractCytokinin-induced gametophore bud formation in the protonema of Ceratodon purpureus was used as a model system to study a possible relationship between cytokinin and polyamines. The levels of free, conjugated and bound to chromatin and ribosomes putrescine, spermidine and spermine in moss growing in a medium with or without kinetin was investigated. Cytokinin elevated (about 2-fold) the level of free, conjugated and bound to the PCA-insoluble fraction putrescine and it was correlated with a 2-fold increase in arginine decarboxylase activity. A higher level of spermine bound to chromatin and spermidine bound to ribosomes m cytokinin-treated protonema can play an important role in cytokinin-evoked stimulation of transcription and translation processes (Gwd£d~ and Szweykowska 1982, Schneider et al. 1988). This suggests that polyamines may be involved in cytokinin mode of action during the induction of gametophore bud formation.This suggestion was additionaly supported by the experiments with inhibitors of putrescine synthesis, D-arginine and canavanine as well as spemfidine synthesis, MGBG, which when added to the medium together with kinetin, reduced or inhibited cytokinin-stimulated gametophore bud formation.

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Polyamines and the Cell Cycle of Catharanthus roseus Cells in Culture

Cited by 33 publications

References 35 publications

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Temporal Regulation of Somatic Embryogenesis by Adjusting Cellular Polyamine Content in Eggplant1

Role of polyamines in cytokinin-dependent physiological processes. III. Changes in polyamine levels during cytokinin-induced formation of gametophore buds in Ceratodon purpureus

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