Polyamines as clinical laboratory tools

Gugliucci, Alejandro

doi:10.1016/j.cccn.2004.02.022

Cited by 120 publications

(86 citation statements)

References 80 publications

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“…Elevated levels of polyamines have been linked to carcinogenesis (9). In vitro experiments have shown this elevation of polyamines to be closely associated and not coincidental (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Difluoromethylornithine on Decreasing Prostate Size and Polyamines in Men: Results of a Year-Long Phase IIb Randomized Placebo-Controlled Chemoprevention Trial

Simoneau

Gerner

Nagle

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Prostate cancer is a major health issue, and prevention of prostate cancer and/or its progression will yield benefits for men. Difluoromethylornithine (DFMO) is an antiproliferative agent, inhibiting ornithine decarboxylase, the first enzyme in the polyamine pathway, and has been studied as a therapeutic and chemopreventive agent. The prostate has high levels of tissue polyamines and has shown sensitivity to DFMO both in vitro and in vivo. Methods: Eighty-one men participated in a 1-year randomized trial of placebo or DFMO. Prostate volume determination and biopsy of the prostate for histology and polyamine content were done at baseline and after 12 months. Other biomarker variables were assessed, including total and free prostate-specific antigen and prostate-specific antigen doubling time.

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Section: Introductionmentioning

confidence: 99%

The Effect of Difluoromethylornithine on Decreasing Prostate Size and Polyamines in Men: Results of a Year-Long Phase IIb Randomized Placebo-Controlled Chemoprevention Trial

Simoneau

Gerner

Nagle

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…1) The key enzyme in mammalian polyamine biosynthesis is ornithine decarboxylase (ODC) which catalyzes the formation of putrescine, the precursor of spermidine and spermine, via decarboxylation of ornithine usually produced by arginase. [1][2][3] In humans, the ODC gene is known to have a restriction fragment length polymorphism when cleaved with Pst I (Hickok), and two ODC alleles as A or G were * To whom correspondence should be addressed: Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20, Daikominami, Higashi-ku, Nagoya 461-8673, Japan. Tel.…”

Section: Introductionmentioning

confidence: 99%

“…3) Among a number of molecular functions performed by polyamines, the role as a direct free radical scavenger is critical in protecting DNA damage from reactive oxygen species. 12) More recently, polyamines were demonstrated to be potent antioxidant and antiinflammatory agents most likely due to their prevention of superoxide generation.…”

Section: Introductionmentioning

confidence: 99%

Association of a Polymorphism in the Ornithine Decarboxylase Gene with Whole Blood Polyamine Concentrations in a Non-smoking Healthy Population

Kondo

Hamajima

Nishio

et al. 2007

JOURNAL OF HEALTH SCIENCE

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Ornithine decarboxylase (ODC) is the rate-limiting key enzyme in the biosynthesis of polyamines which have been confirmed to possess potent antioxidant and antiglycating properties. The aim of the present study was to examine the relationship of the ODC polymorphism and circulating whole blood polyamine concentrations. The study subjects were non-smoking, healthy men (n = 30) and women (n = 30) aged 55-69 years with equal numbers of AA, GA, and GG genotypes of the ODC gene, who were randomly recruited from 607 health checkup examinees. The whole blood polyamines (spermidine and spermine) were determined by high-performance liquid chromatography and were adjusted for hematocrit. The difference in the adjusted polyamines across the ODC gene was statistically tested using analysis of covariance. Women homozygous for the A-allele showed significantly higher spermidine levels than those with other genotypes. No such association was found among men, and spermine showed no association with the ODC genotype. In conclusion, the ODC polymorphism is significantly associated with whole blood polyamines in women. The ODC gene seems to be expressed more actively among female A-allele homozygotes than women carrying the G-allele.

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“…Polyamines are ubiquitous in living organisms and play important roles in cell growth, division, and differentiation [1,2]. The basal levels of polyamine concentrations in normal cells are highly regulated by biosynthetic enzymes; therefore, determining their endogenous concentrations is well-known method for diagnosing a number of cancer and ageassociated diseases because the concentrations in neoplastic cells are higher than those in normal cells [3,4].…”

Section: Introductionmentioning

confidence: 99%

Multi-Perfluoroalkyl Derivatization of Polyamines for Selective Liquid Chromatography-Tandem Mass Spectrometric Analysis Utilizing Fluorous Affinity

et al. 2017

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A novel derivatization method for the selective and sensitive liquid chromatography (LC)-tandem mass spectrometric (MS/MS) analysis of polyamines (putrescine, cadaverine, spermidine, and spermine) was developed. In this study, to utilize the specific affinity between perfluoroalkyl compounds, called 'fluorous' affinity, two to four amino groups in each polyamine molecule were transformed with a relatively short perfluoroalkyl reagent, N-succinimidyl 4,4,5,5,6,6,7,7,8,8,9,9,9-tridecafluorononanoate to achieve the corresponding diperfluoroalkyl-to tetraperfluoroalkyl-derivatives. Compared with the non-fluorous compounds, the fluorophilicity of the multi-fluorous derivatives was sufficient for realizing strong retention on an LC column with a perfluoroalkyl-modified stationary phase. Furthermore, sensitive analysis of these derivatives could be performed using the multiple reaction monitoring mode in positive electrospray ionization-MS/MS. The limits of detection of the polyamines were in the range of 0.31-1.4 nM. The method was validated using human plasma samples. Although the recoveries from spiked human plasma after ultrafiltration were in the range of 66.4-103%, the derivatives could be determined without interference from matrix effects because of their selective retention on the column, which excluded non-fluorous biological matrix components, such as phospholipids. Therefore, this sensitive and selective analysis method was useful for the determination of trace amounts of polyamines in human plasma.

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Polyamines as clinical laboratory tools

Cited by 120 publications

References 80 publications

The Effect of Difluoromethylornithine on Decreasing Prostate Size and Polyamines in Men: Results of a Year-Long Phase IIb Randomized Placebo-Controlled Chemoprevention Trial

The Effect of Difluoromethylornithine on Decreasing Prostate Size and Polyamines in Men: Results of a Year-Long Phase IIb Randomized Placebo-Controlled Chemoprevention Trial

Association of a Polymorphism in the Ornithine Decarboxylase Gene with Whole Blood Polyamine Concentrations in a Non-smoking Healthy Population

Multi-Perfluoroalkyl Derivatization of Polyamines for Selective Liquid Chromatography-Tandem Mass Spectrometric Analysis Utilizing Fluorous Affinity

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