Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation

Veríssimo, Denusa Moreira; Leitão, Renata F. C.; Ribeiro, Ronaldo A.; Figueiró, S.D.; Sombra, A. S. B.; Góes, J.C.; Brito, Gerly Anne Castro

doi:10.1016/j.actbio.2010.04.012

Cited by 69 publications

(48 citation statements)

References 35 publications

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“…48 Crosslinking provides collagen scaffolds with an additional benefit, because by varying the degree of crosslinking present in and between collagen molecules, it is possible to control the rate of in vivo degradation. 19,49,50 Ideally, scaffolds should be designed with a slow enough degradation rate in order to maintain mechanical viability long enough to facilitate and support cell activity during the remodeling process. However, it is important that scaffolds do degrade over time in order to transfer function and transmit mechanical forces to the newly formed tissue and to prevent the requirement for further surgery to facilitate removal.…”

Section: Dehydrothermal Processingmentioning

confidence: 99%

Collagen scaffolds for orthopedic regenerative medicine

Cunniffe

O’Brien

2011

JOM

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How would you……describe the overall signifi cance of this paper?Collagen and collagen-based scaffolds offer distinct advantages when selected as biomaterials for use across a broad spectrum of regenerative medicine applications. However, relatively poor mechanical properties are often perceived to limit their usefulness for orthopedic applications. These problems can be overcome through enhanced crosslinking mechanisms or through the addition of a second, stiffer phase such as hydroxyapatite, thus allowing tailored composite scaffolds to meet specifi c tissue requirements. This overview will highlight the current state of the art of these scaffolds, and consider the exciting prospects and future directions of collagen-based technologies for orthopedic regenerative medicine.…describe this work to a materials science and engineering professional with no experience in your technical specialty?Disease, injury and trauma can cause damage and degeneration of tissues and organs in the human body, which requires treatments to facilitate their repair, replacement or regeneration. Tissue engineering aims to regenerate damaged tissues instead of replacing them, by implanting biological substitutes that restore, sustain, or improve tissue function. Tissue engineering relies considerably on the use of porous 3-D scaffolds to provide a suitable environment for the regeneration of tissues and organs. These scaffolds essentially provide a template for new tissue formation and are either seeded with cells prior to implantation or are implanted directly into the injured site and the body's own cells populate the construct to facilitate healing. This review article focuses on the use of collagen and collagen-based scaffolds for tissue engineering applications. Overview biomaterials for regenerative medicineCollagen and collagen-based scaffolds offer distinct advantages when selected as biomaterials for use across a broad spectrum of regenerative medicine applications. However, relatively poor mechanical properties are often perceived to limit their usefulness for orthopedic applications. These problems can be overcome through enhanced crosslinking mechanisms or through the addition of a second, stiffer phase such as hydroxyapatite, thus allowing tailored composite scaffolds to meet specifi c tissue requirements. This overview will highlight the current state of the art of these scaffolds, and consider the exciting prospects and future directions of collagen-based technologies for orthopedic regenerative medicine.

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Section: Dehydrothermal Processingmentioning

confidence: 99%

Collagen scaffolds for orthopedic regenerative medicine

Cunniffe

O’Brien

2011

JOM

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“…The process of crosslinking of collagen with glutaraldehyde and the amount of glutaraldehyde used, has been improved to withdraw its cytotoxicity [35]. Verissimo et al performed a study about progressive crosslinking with glutaraldehyde in 2010 [36]. A polyanionic collagen membrane was prepared with consecutive cycles of mineralization with hydroxyapatite.…”

Section: Collagenmentioning

confidence: 99%

Membranes for periodontal tissues regeneration

Babo

Pires

Reis

et al. 2014

Ciência & Tecnologia dos Materiais

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“…41 Consistent with these data, we have previously demonstrated that GA cross-linked membranes reduce the inflammatory response in the rat subcutaneous implant model. 13 …”

Section: 27mentioning

confidence: 99%

“…The preparation of these PAC membranes as well as the methods for the mineralization and cross-linking with GA was described previously. 13 The membranes were manufactured by LOCEM, in the Physics Department of the Federal University of Ceará (Brazil). A collagen membrane of demineralized bovine cortical bone (Genderm, Genius, Baumer S.A., Brazil) was used as a control.…”

Section: Collagen Membranementioning

confidence: 99%

“…12 Consistent with these reports, we recently demonstrated that the subcutaneous implantation in rats of mineralized polyanionic collagen (PAC) membranes cross-linked with GA led to a reduced inflammatory response and a lack of membrane resorption compared with implantation of membranes that were not crosslinked. 13 The critical-size rat calvarium defect model has been used in several investigations to evaluate guided bone regeneration. [14][15][16] In rats, 5-mm calvarial defects are regarded as critical-size defects (CSDs).…”

mentioning

confidence: 99%

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Guided bone regeneration produced by new mineralized and reticulated collagen membranes in critical-sized rat calvarial defects

Veríssimo

Leitão

Figueiró

et al. 2014

Exp Biol Med (Maywood)

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The aim of this study was to evaluate the bone regenerative effect of glutaraldehyde (GA) cross-linking on mineralized polyanionic collagen membranes in critical-sized defects on rat calvarias. Bone calvarial defects were induced in Wistar rats, which were then divided into five groups: a sham group; a control group, which received a commercial membrane; and GA, 25GA, and 75GA groups, which received one of three different polyanionic collagen membranes mineralized by 0, 25, or 75 hydroxyapatite cycles and then cross-linked by GA. Bone formation was evaluated based on digital radiography and computerized tomography. Histological analyses were performed 4 and 12 weeks after the surgical procedure to observe bone formation, membrane resorption, and fibrous tissue surrounding the membranes. Measurement of myeloperoxidase activity, tumor necrosis factor alpha, and interleukin 1beta production was performed 24 h after surgery. The percentage of new bone formation in the GA, 25GA, and 75GA groups was higher compared with the control and sham groups. In the GA and 25 GA groups, the membranes were still in place and were contained in a thick fibrous capsule after 12 weeks. No significant difference was found among the groups regarding myeloperoxidase activity and interleukin 1beta levels, although the GA, 25GA, and 75GA groups presented decreased levels of tumor necrosis factor alpha compared with the control group. These new GA cross-linked membranes accelerated bone healing of the calvarium defects and did not induce inflammation. In addition, unlike the control membrane, the experimental membranes were not absorbed during the analyzed period, so they may offer advantages in large bone defects where prolonged membrane barrier functions are desirable.

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Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation

Cited by 69 publications

References 35 publications

Collagen scaffolds for orthopedic regenerative medicine

Collagen scaffolds for orthopedic regenerative medicine

Membranes for periodontal tissues regeneration

Guided bone regeneration produced by new mineralized and reticulated collagen membranes in critical-sized rat calvarial defects

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