Polyanionic Polysaccharides Reduce Intra-abdominal Adhesion and Abscess Formation in a Rat Peritonitis Model

Reijnen, Michel M.P.J.; Skrabut, E.M.; Postma, V.A.; Burns, James W.; Goor, Harry van

doi:10.1006/jsre.2001.6288

Cited by 43 publications

(31 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Modified HA derivates are presently used to reduce the number and severity of post-surgical adhesions [10][11][12]. The question has been raised as to whether these derivates would prevent tumour implantation and growth at the traumatized peritoneum, because of the presumed similarity between pathogenesis of adhesions and tumour implantation [5,8].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronan-Based Antiadhesive Membrane Has No Major Effect on Intraperitoneal Growth of Colonic Tumour Cells

Sikkink¹,

Postma²,

Reijnen³

et al. 2004

Eur Surg Res

View full text Add to dashboard Cite

Background: A relationship between post-surgical adhesion formation and peritoneal tumour implantation has been proposed. Hyaluronan (HA)-based agents reduce adhesion formation, but the effect on peritoneal tumour is not established. This study investigated the influence of a HA-containing agent on intraperitoneal tumour in an experimental model. Methods: 66 Balb/c mice underwent laparotomy and damage was inflicted to the parietal peritoneum. The animals were randomized into five groups. Groups 1 and 2 received HA-carboxymethylcellulose bioresorbable membrane and no treatment, respectively. Mice in groups 3–5 were injected intraperitoneally with 10⁵ colon 26-B cells after the laparotomy. Treatment consisted of HA membrane, no HA agent and placement of HA membrane on the non-traumatized peritoneal wall, respectively. Animals were killed after 14 days; adhesions were scored in groups 1 and 2, and the tumour mass in groups 3–5. 45 Wag/Rij rats underwent the same procedures and treatment as mice in groups 3–5. In rats, 10⁶ CC-531 cells were injected. Rats were killed after 3 weeks and the tumour mass was scored. Results: HA membrane resulted in a significant reduction of adhesions, but had no major effect on the intraperitoneal tumour mass in mice and rats. Conclusion: HA-carboxymethylcellulose bioresorbable membrane has no major effect on intraperitoneal tumour implantation and growth in an experimental model.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Hyaluronan (HA) derivates reduce post-surgical ad-hesion formation [10][11][12]. The adhesion-reducing capacity of this polyanionic polysaccharide seems to be the result of mechanical separation, as well as biological effects on peritoneal repair mechanisms [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan-Based Antiadhesive Membrane Has No Major Effect on Intraperitoneal Growth of Colonic Tumour Cells

Sikkink¹,

Postma²,

Reijnen³

et al. 2004

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…Barrier devices have been tested or commercialized in various forms including polymer solutions [43][44][45], solid membranes [46], pre-formed [47][48][49] or in situ crosscrosslinkable hydrogels [41,50,51]. Solutions, mostly consisting of viscous polysaccharides, are applied by a variety of methods (spraying, pouring, extrusion from a container) at the end of the surgical procedure.…”

Section: Barrier Devicesmentioning

confidence: 99%

Polymers in the prevention of peritoneal adhesions

Yeo

Kohane

2008

European Journal of Pharmaceutics and Biopharmaceutics

108

View full text Add to dashboard Cite

Peritoneal adhesions are serious complications of surgery, and can result in pain, infertility, and potentially lethal bowel obstruction. Pharmacotherapy and barrier devices have reduced adhesion formation to varying degrees in preclinical studies or clinical trials; however, complete prevention of adhesions remains to be accomplished. We and others have hypothesized that the limitations of the two approaches could be overcome by combining their strengths in the context of controlled drug delivery. Here we review the role of polymeric systems in the prevention of peritoneal adhesions, with an emphasis on our recent work in developing and applying polymeric drug delivery systems such as nano-or microparticles, hydrogels, and hybrid systems for peritoneal use.

show abstract

“…The adhesion-reducing capacity of this solution is well established in animals [8,9,22]. Reijnen et al [23] reported that intraperitoneal treatment with 8 ml of 1.7% CMC solution significantly reduced both adhesion and abscess formation in a rat peritonitis model. In the present study, we found that the lower concentration of 0.2% CMC also had an adhesion-and abscess-reducing capacity.…”

Section: Discussionmentioning

confidence: 99%

“…The swabs were immediately introduced into medium and cultured semiquantitatively in aerobic and anaerobic conditions [23].…”

Section: Preparation Of Materialsmentioning

confidence: 99%

The Effect of Polysaccharides and Carboxymethylcellulose Combination to Prevent Intraperitoneal Adhesion and Abscess Formation in a Rat Peritonitis Model

Bae

Jin

Jang

2004

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Polysaccharides isolated from fungi, Phellinus spp. is well-known material with anti-tumor and anti-inflammatory properties. We have assessed the adhesion-and abscess-reducing capacity of carboxymethylcellulose (CMC) and polysaccharides from Phellinus spp. combination in a rat peritonitis model. In 72 Sprague-Dawley rats, experimental peritonitis was induced by means of the ce cal ligation and puncture model (CLP). After 24 hr, the abdomen was reopened and the ligated cecum was resected. Peritoneal fluid samples were taken for microbiological examination. Rats were randomly assigned to 6 groups: ringer lactate solution (RL group), polysaccharides from Phellinus gilvus (PG group) and Phellinus linteus (PL group), carboxymethylcellulose (CMC group), and their combinations (PG+CMC and PL+CMC groups). Adhesions and abscesses were noted at day 7 after CLP. RT-PCR assay for urokinase-type plasminogen activator (uPA), its cellular receptor (uPAR), and tumor necrosis factor (TNF)-α was performed to assess the cecal tissue. Microbiological examination showed polymicrobial bacterial peritonitis. Adhesion formation was significantly reduced in PG+CMC and PL+CMC groups (P<0.05). The incidence of abscesses was reduced in all treated groups except the RL group (P<0.05). uPA, uPAR, and TNF-α mRNA were highly expressed in the PG+CMC and PL+CMC groups, as compared to the RL group. We concluded that the combination of polysaccharides and CMC had significant adhesion-and abscess-reducing effects compared with their single treatment and the effects may act by modifying the fibrinolytic capacity of uPA, uPAR and TNF-α produced from activated macrophages in a rat peritonitis model.

show abstract

Polyanionic Polysaccharides Reduce Intra-abdominal Adhesion and Abscess Formation in a Rat Peritonitis Model

Cited by 43 publications

References 18 publications

Hyaluronan-Based Antiadhesive Membrane Has No Major Effect on Intraperitoneal Growth of Colonic Tumour Cells

Hyaluronan-Based Antiadhesive Membrane Has No Major Effect on Intraperitoneal Growth of Colonic Tumour Cells

Polymers in the prevention of peritoneal adhesions

The Effect of Polysaccharides and Carboxymethylcellulose Combination to Prevent Intraperitoneal Adhesion and Abscess Formation in a Rat Peritonitis Model

Contact Info

Product

Resources

About