Polydatin exerts anti-tumor effects against renal cell carcinoma cells via induction of caspase-dependent apoptosis and inhibition of the PI3K/Akt pathway

Jin, Yi; Xin, Limin; Zhou, Changchun; Ren, Yu

doi:10.2147/ott.s180785

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…In fact, biomaterials have an important dual purpose: to release the antitumor drugs in the bone at the place of surgical intervention and to mimic the bone microenvironment by inducing tissue regeneration [20]. Polydatin, a natural glycosylate precursor of resveratrol, has been reported to have an antitumor effect by inhibiting the PI3K/Akt and PDGF/Akt pathway [29][30][31][32]. It was reported that polydatin inhibited the proliferation and promoted the apoptosis of OS cells through upregulation of the Bax/Bcl-2 ratio and attenuation of β-catenin signaling [33].…”

Section: Discussionmentioning

confidence: 99%

Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation

et al. 2022

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Polycaprolactone nanofibers are used as scaffolds in the field of tissue engineering for tissue regeneration or drug delivery. Polycaprolactone (PCL) is a biodegradable hydrophobic polyester used to obtain implantable nanostructures, which are clinically applicable due to their biological safety. Polydatin (PD), a glycosidic precursor of resveratrol, is known for its antioxidant, antitumor, antiosteoporotic, and bone regeneration activities. We aimed to use the osteogenic capacity of polydatin to create a biomimetic innovative and patented scaffold consisting of PCL-PD for bone tissue engineering. Both osteosarcoma cells (Saos-2) and mesenchymal stem cells (MSCs) were used to test the in vitro cytocompatibility of the PD-PCL scaffold. Reverse-phase (RP) HPLC was used to evaluate the timing release of PD from the PCL-PD nanofibers and the MTT assay, scanning electron microscopy, and alkaline phosphatase (ALP) activity were used to evaluate the proliferation, adhesion, and cellular differentiation in both osteosarcoma and human mesenchymal stem cells (MSCs) seeded on PD-PCL nanofibers. The proliferation of osteosarcoma cells (Saos-2) on the PD-PCL scaffold decreased when compared to cells grown on PLC nanofibers, whereas the proliferation of MSCs was comparable in both PCL and PD-PCL nanofibers. Noteworthy, after 14 days, the ALP activity was higher in both Saos-2 cells and MSCs cultivated on PD-PCL than on empty scaffolds. Moreover, the same cells showed a spindle-shaped morphology after 14 days when grown on PD-PCL as shown by SEM. In conclusion, we provide evidence that nanofibers appropriately coated with PD support the adhesion and promote the osteogenic differentiation of both human osteosarcoma cells and MSCs.

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Section: Discussionmentioning

confidence: 99%

Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation

et al. 2022

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“…In contrast, 2-Cl-MGV-1 achieved a significant protective effect on the same biological processes (Figures 1, 4 One of the main intracellular functions of TSPO is its regulatory role in apoptotic cell death [12,13]. Caspase-dependent apoptosis was previously demonstrated to be sensitive to TSPO ligands [24,25,43,44]. In this study, caspase-dependent apoptosis was expressed by the significant elevation in cell death levels along with the apoptotic markers caspases 3, 8 and 9 following 60 min of CS exposure.…”

Section: Discussionmentioning

confidence: 99%

The TSPO Ligands MGV-1 and 2-Cl-MGV-1 Differentially Inhibit the Cigarette Smoke-Induced Cytotoxicity to H1299 Lung Cancer Cells

Zeineh

Nagler

Gabay

et al. 2021

Biology

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TSPO is involved in cigarette smoke (CS)-induced cellular toxicity, which may result in oral and pulmonary diseases and lung cancer. H1299 lung cancer cells were exposed directly to CS. The H1299 cells were pretreated with our TSPO ligands MGV-1 and 2-Cl-MGV-1 (Ki = 825 nM for both) at a concentration of 25 µM 24 h prior to CS exposure. Cell death and apoptotic markers were measured, in addition to TSPO expression levels, ATP synthase activity, generation of reactive oxygen species (ROS), depolarization of mitochondrial membrane potential (ΔΨm), cAMP and LDH levels. Pretreatment with MGV-1 and 2-Cl-MGV-1 (25 µM), 24 h prior to CS exposure, differentially attenuated the CS-induced cellular insult as well as cell death in H1299 lung cancer cells. These protective effects included prevention of ATP synthase reversal, ROS generation, depolarization of the mitochondrial membrane and elevation in LDH. The preventive efficacy of 2-Cl-MGV-1 was superior to that achieved by MGV-1. Both ligands did not prevent the elevation in cAMP. These findings may indicate a mild protective effect of these TSPO ligands in CS-related pulmonary and keratinocyte cellular pathology.

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“…Polydatin has showed anticancer effects against various tumours in laboratory, including breast cancer, liver cancer and renal cancer (55)(56)(57) . Efforts in the field of bone cancer are mainly devoted to osteosarcoma, and a broad spectrum of cell responses have been found to be involved in the anti-osteosarcoma process of polydatin (Fig.…”

Section: Anticancer Effects Of Polydatin On Bonesmentioning

confidence: 99%

Protective effects of polydatin against bone and joint disorders: thein vitroandin vivoevidence so far

et al. 2023

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Polydatin is an active polyphenol displaying multifaceted benefits. Recently, growing studies have noticed its potential therapeutic effects on bone and joint disorders (BJDs). Therefore, this article reviews recent in vivo and in vitro progress on the protective role of polydatin against BJDs. An insight into the underlying mechanisms is also presented. It was found that polydatin could promote osteogenesis in vitro, and symptom improvements have been disclosed with animal models of osteoporosis, osteosarcoma, osteoarthritis, and rheumatic arthritis. These beneficial effects obtained in laboratory could be mainly attributed to the bone metabolism-regulating, anti-inflammatory, antioxidative, apoptosis-regulating, and autophagy-regulating functions of polydatin. However, studies on human subjects with BJDs that can lead to early identification of the clinical efficacy and adverse effects of polydatin have not been reported yet. Accordingly, this review would serve as a starting point for pursuing clinical trials. Additionally, future emphasis should also be devoted to the low bioavailability and prompt metabolism nature of polydatin. In summary, well-designed clinical trials of polydatin in BJD patients are in demand, and its pharmacokinetic nature must be taken into account.

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Polydatin exerts anti-tumor effects against renal cell carcinoma cells via induction of caspase-dependent apoptosis and inhibition of the PI3K/Akt pathway

Cited by 8 publications

References 30 publications

Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation

Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation

The TSPO Ligands MGV-1 and 2-Cl-MGV-1 Differentially Inhibit the Cigarette Smoke-Induced Cytotoxicity to H1299 Lung Cancer Cells

Protective effects of polydatin against bone and joint disorders: thein vitroandin vivoevidence so far

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