Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1 (Sirt1)

Abstract

The aim of the present study was to investigate the beneficial effect of polydatin (PD), the glycoside form of resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries of Korean Hanwoo cows and cultured until Day 8 in a humidified atmosphere of 5% CO in air at 38.5°C. Protein and gene expression levels were determined through confocal microscopy and reverse transcription-polymerase chain reaction respectively, whereas the number of total and apoptotic cells in Day 8 blastocysts was determined using Hoechst 33342 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling. Of the different concentrations of PD (0.5, 1.0 and 2.0µM) added to the IVM medium, only 1.0µM PD significantly improved blastocyst development. Immunofluorescence analysis confirmed that protein levels of sirtuin 1 (Sirt1) increased significantly (P<0.05) after PD treatment, whereas levels of reactive oxygen species (ROS) were significantly (P<0.05) decreased, as evidenced by reductions in 8-oxoguanine immunoreactivity. Similarly, protein levels of nuclear factor (NF)-κB and cyclo-oxygenase (COX)-2 were significantly (P<0.05) lower in the PD-treated group than in the control group. Treatment with 1.0µM PD reduced gene expression of BCL2-associated X protein, inducible nitric oxide synthase, COX2 and Nfkb, but increased the expression of Sirt1, supporting the immunofluorescence data. PD possesses antioxidant activity and is useful for embryo development in vitro. We conclude that supplementation of IVM medium with PD improves embryo developmental competence via Sirt1.

Improved developmental competence in embryos treated with lycopene during in vitro culture system

“…The data were normalized to an average of two background regions for each projection. The average intensity of each protein was considered for statistical analysis (Khan et al, ; Khan, Kim, et al, ; Khan, Lee, et al, ; Mesalam, Khan, et al, ).…”

“…Embryonic culture conditions also influence lipid metabolism and the oxidative stress response at the gene‐expression level, which is particularly evident when comparing the transcriptomes of blastocysts generated in vivo versus in vitro (Côté et al, ; Gad et al, ; Plourde et al, ). For example, embryos developed in vitro usually possess markedly more lipids than in vivo controls, which increase the abundance of substrate for damaging lipid peroxidation (De La Torre‐Sanchez, Gardner, Preis, Gibbons, & Seidel, ; De La Torre‐Sanchez, Preis, Seidel, ; Khan, Kim, et al, ; Seidel, ). When lipid‐reducing chemicals were added to culture media, embryo development, and quality were improved (De La Torre‐Sanchez, Gardner, et al, ; De La Torre‐Sanchez, Preis, et al, ; Takahashi et al, ).…”

The Role of Resveratrol in Mammalian Reproduction

“…When supplemented to in vitro maturation (IVM) medium, polydatin, a glycosidic form of resveratrol, improved embryo development, increasing SIRT1 protein and decreasing reactive oxygen species (ROS) [ 48 ]. In the same work, embryo protein levels of nuclear factor NF-kB and cyclooxygenase (COX2) were significantly lower when polydatin was added to the culture medium [ 48 ].…”

“…When supplemented to in vitro maturation (IVM) medium, polydatin, a glycosidic form of resveratrol, improved embryo development, increasing SIRT1 protein and decreasing reactive oxygen species (ROS) [ 48 ]. In the same work, embryo protein levels of nuclear factor NF-kB and cyclooxygenase (COX2) were significantly lower when polydatin was added to the culture medium [ 48 ]. Since NFkB and especially COX2 play a pivotal role in inflammation, the authors supposed that this resveratrol analogue (polydatin) might have a beneficial effect on embryo development by decreasing their expression, thereby reducing any inflammatory processes in progress [ 48 ].…”

“…In the same work, embryo protein levels of nuclear factor NF-kB and cyclooxygenase (COX2) were significantly lower when polydatin was added to the culture medium [ 48 ]. Since NFkB and especially COX2 play a pivotal role in inflammation, the authors supposed that this resveratrol analogue (polydatin) might have a beneficial effect on embryo development by decreasing their expression, thereby reducing any inflammatory processes in progress [ 48 ]. Using immunofluorescence and Western blot techniques, Wang et al [ 49 ] evidenced the presence of SIRT1 in bovine granulosa cells, cumulus cells, oocytes, and blastocysts.…”