Polydiacetylene Liposomes Functionalized with Sialic Acid Bind and Colorimetrically Detect Influenza Virus

Reichert, A.; Nagy, Jon O.; Spevak, Wayne; Charych, Deborah H.

doi:10.1021/ja00107a032

Cited by 269 publications

(202 citation statements)

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“…3b), while the rapid reversibility was maintained even aer dozens of thermal cycles (Fig. 3c, CR (colorimetric response), 20 (B 0 À B temperature )/B 0 Â 100%, B temperature ¼ A 630 /(A 630 + A 540 ), where B 0 is the initial value and A wavelength is the absorbance at the corresponding wavelength). Furthermore, different heating temperatures corresponded to different recovered spectra which, in our opinion, is due to a partly irreversible conformational transition in the rst heating stage (Fig.…”

Section: Thermochromism and Mechanical Propertiesmentioning

confidence: 95%

Ultrafast and reversible thermochromism of a conjugated polymer material based on the assembly of peptide amphiphiles

et al. 2014

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Section: Thermochromism and Mechanical Propertiesmentioning

confidence: 95%

Ultrafast and reversible thermochromism of a conjugated polymer material based on the assembly of peptide amphiphiles

et al. 2014

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“…During the polymerization, adjacent diacetylene units, transfer into a polyconjugated polydiacetylenic polymer backbone with remarkable optical properties. Different factors like temperature [2], pH [4], organic solvent [1] and mechanical stress [12] trigger Colour transition from blue to red due to conformational changes in the conjugated backbone in Polydiacetylene assemblies.The best examples of Polydiacetylene biosensors are carbohydrate functionalized PDA for the detection of the influenza virus, cholera toxin [5,21,27], and E. coli. The topochemically polymerization is achieved by self assembly of the diacetylene entities in ordered form depending on their molecular architecture and nature.…”

Section: Introduction:-mentioning

confidence: 99%

Stable Drug Delivery Systems: Peg-Polydiacetylene Liposomes.

Swapna¹

2017

IJAR

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“…PDA-based biosensors have been proven to be sensitive to a wide range of analytes, such as proteins, lipophilic enzymes, bacteria, viruses, and pharmacologically active compounds. [9][10][11][12][13] However, PDA vesicles (PDAVs) are fragile and collapse easily, which limits their practical applications. To improve the versatility of PDAVs and enable multiplex detection, immobilization of the vesicles on solid substrates is needed.…”

Section: Introductionmentioning

confidence: 99%

A reinforced composite structure composed of polydiacetylene assemblies deposited on polystyrene microspheres and its application to H5N1 virus detection

Wu¹,

Luo²,

He³

et al. 2013

IJN

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Abstract:In this study, we immobilized polydiacetylene vesicles (PDAVs) onto the surface of polystyrene (PS) microspheres (1 µm in diameter) by using both electrical charge and conjugated forces to form a reinforced composite structure. These reinforced complexes could be easily washed, separated by centrifugation, and resuspended by gentle agitation. After passing through a narrow 200 µm-diameter channel, the composite structures maintained their original shape, demonstrating their resilience and potential for use in microfluidic technologies. The number of PDAVs in the composite structure could be mediated by changing the extent of layer deposition, which affected the sensitivity of detection. It showed that PDAVs did not change their blue color after addition of detecting probes such as anti-H5N1, which was of great importance in the fabrication and modification of stable color-changeable biosensors based on PDAVs. By conjugating anti-H5N1 antibodies to the PS@PDAV via N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide chemistry, a stable blue complex, anti-H5N1 microsphere (PS@PDAV-anti-H5N1) was formed. A target antigen of H5N1 (HAQ [H5N1 strain A/ environment/Qinghai/1/2008{H5N1} in clade 0]) was detected by PS@PDAV-anti-H5N1. At an optimal PDAV deposition level of three layers, the limit of detection was determined to be approximately 30 ng/mL of HAQ by using optical spectrum measurement and visual inspection, meeting the needs of fast and simple color-changeable detection. However, a much lower limitation of detection (1 ng/mL) was able to be obtained using laser-scanning confocal microscopy, which could be compared with the results obtained with other sophisticated equipment.

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Polydiacetylene Liposomes Functionalized with Sialic Acid Bind and Colorimetrically Detect Influenza Virus

Cited by 269 publications

References 0 publications

Ultrafast and reversible thermochromism of a conjugated polymer material based on the assembly of peptide amphiphiles

Ultrafast and reversible thermochromism of a conjugated polymer material based on the assembly of peptide amphiphiles

Stable Drug Delivery Systems: Peg-Polydiacetylene Liposomes.

A reinforced composite structure composed of polydiacetylene assemblies deposited on polystyrene microspheres and its application to H5N1 virus detection

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