Polydiacetylene Nanovesicles as Carriers of Natural Phenylpropanoids for Creating Antimicrobial Food-Contact Surfaces

Abstract

The ultimate goal of this study was developing antimicrobial food-contact materials based on natural phenolic compounds using nanotechnological approaches. Among the methyl-β-cyclodextrin-encapsulated phenolics tested, curcumin showed by far the highest activity toward Escherichia coli with a minimum inhibitory concentration of 0.4 mM. Curcumin was enclosed in liposome-type polydiacetylene/phosholipid nanovesicles supplemented with N-hydroxysuccinimide and glucose. The fluorescence spectrum of the nanovesicles suggested that curcumin was located in their bilayer region. Free-suspended nanovesicles tended to bind to the bacterial surface and demonstrated bactericidal activity toward Gram-negative (E. coli) and vegetative cells of Gram-positive (Bacillus cereus) bacteria reducing their counts from 5 log CFU mL(-1) to an undetectable level within 8 h. The nanovesicles were covalently bound to silanized glass. Incubation of E. coli and B. cereus with nanovesicle-coated glass resulted in a 2.5 log reduction in their counts. After optimization this approach can be used for controlling microbial growth, cross-contamination, and biofilm formation on food-contacting surfaces.

The mode of antimicrobial action of curcumin depends on the delivery system: monolithic nanoparticles vs. supramolecular inclusion complex

“…The uorescent emission wavelength of curcumin is known to be dependent on the polarity of the environment and to be shiing towards longer wavelengths with increasing solvent polarity. 11 The uorescence spectrum of bacterial cells treated with curcumin-CD closely coincided with the spectrum of the complex itself with an emission maximum at 511 nm (Fig. 3B) reecting the curcumin situated in the hydrophobic inner core of cyclodextrin molecule and supporting the suggestion that CCD is taken-up by bacterial cells as a whole complex.…”

Therapeutic Applications of Curcumin Nanoformulations

“…An aqueous dispersion of wet milling nanocurcumin exhibited potent activity against a number bacteria (S. aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Penicillium notatum, and Aspergillus niger); however, it was more effective against Gram-positive bacteria than Gram-negative bacteria (112). Curcumin nanovesicles efficiently bind to the surface of bacteria and reduce their counts from 5 log CFU mL −1 to an undetectable level, suggesting a novel approach for controlling microbial growth, cross-contamination, and biofilm formation (113).…”

Effect of Lipid Composition onIn VitroRelease and Skin Deposition of Curcumin Encapsulated Liposomes

“…Liposomal encapsulation has improved numerous important properties of curcumin with potential applications in many different fields. For instance, curcumin encapsulated liposomes have been utilized in developing antimicrobial surfaces for the food industry [11]. Curcumin nanoliposomes coated with chitosan were considered suitable for applications in the food industry by Shin and coworkers, based on their increased bioavailability, mucoadhesive properties, and storage stability [12].…”