Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Sun, Lin; Lehnert, Thomas; Gijs, Martin A. M.; Li, Songjing

doi:10.1039/d2lc00366j

Cited by 6 publications

(2 citation statements)

References 65 publications

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“…However, SAW-based acoustic streaming typically requires a SAW transducer outside the microchannel, which drastically increases the device footprint. The same issue is found in other microfluidic devices with active acoustic or photoacoustic streaming produced by lasers or piezoelectric transducers [11,12].…”

Section: Introductionsupporting

confidence: 58%

Acoustic Streaming Efficiency in a Microfluidic Biosensor with an Integrated CMUT

et al. 2023

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The effect of microchannel height on acoustic streaming velocity and capacitive micromachined ultrasound transducer (CMUT) cell damping was investigated. Microchannels with heights ranging from 0.15 to 1.75 mm were used in experiments, and computational microchannel models with heights varying from 10 to 1800 micrometers were simulated. Both simulated and measured data show local minima and maxima of acoustic streaming efficiency associated with the wavelength of the `bulk acoustic wave excited at 5 MHz frequency. Local minima occur at microchannel heights that are multiples of half the wavelength (150 μm), which are caused by destructive interference between excited and reflected acoustic waves. Therefore, microchannel heights that are not multiples of 150 μm are more favorable for higher acoustic streaming effectiveness since destructive interference decreases the acoustic streaming effectiveness by more than 4 times. On average, the experimental data show slightly higher velocities for smaller microchannels than the simulated data, but the overall observation of higher streaming velocities in larger microchannels is not altered. In additional simulation, at small microchannel heights (10–350 μm), local minima at microchannel heights that are multiples of 150 μm were observed, indicating the interference between excited and reflected waves and causing acoustic damping of comparatively compliant CMUT membranes. Increasing the microchannel height to over 100 μm tends to eliminate the acoustic damping effect as the local minima of the CMUT membrane swing amplitude approach the maximum value of 42 nm, which is the calculated amplitude of the freely swinging membrane under the described conditions. At optimum conditions, an acoustic streaming velocity of over 2 mm/s in a 1.8 mm-high microchannel was achieved.

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Section: Introductionsupporting

confidence: 58%

Acoustic Streaming Efficiency in a Microfluidic Biosensor with an Integrated CMUT

et al. 2023

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“…However, this approach requires the introduction of sheath flow focusing before the cells reach the acoustic manipulation region, which may contaminate the sample as well as affect cell activity. Compared to SAW methods, BAW devices(operating frequencies in the 1-1000 KHz [31]) are often combined with bubbles or solid microstructures to achieve versatile and efficient sample manipulation capabilities at lower fabrication costs as well as power requirements. Wang et al [32] achieved selective capture, accumulation, and release of particles depending on the size difference by combining a stabilized background flow with a microbubble acoustic microfluidic technique.…”

Section: Introductionmentioning

confidence: 99%

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Lin,

Zhu,

et al. 2024

Micromachines

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The precise isolation of circulating tumor cells (CTCs) from blood samples is a potent tool for cancer diagnosis and clinical prognosis. However, CTCs are present in extremely low quantities in the bloodstream, posing a significant challenge to their isolation. In this study, we propose a non-contact acoustic micropillar array (AMPA) chip based on acoustic streaming for the flexible, label-free capture of cancer cells. Three shapes of micropillar array chips (circular, rhombus, and square) were fabricated. The acoustic streaming characteristics generated by the vibration of microstructures of different shapes are studied in depth by combining simulation and experiment. The critical parameters (voltage and flow rate) of the device were systematically investigated using microparticle experiments to optimize capture performance. Subsequently, the capture efficiencies of the three micropillar structures were experimentally evaluated using mouse whole blood samples containing cancer cells. The experimental results revealed that the rhombus microstructure was selected as the optimal shape, demonstrating high capture efficiency (93%) and cell activity (96%). Moreover, the reversibility of the acoustic streaming was harnessed for the flexible release and capture of cancer cells, facilitating optical detection and analysis. This work holds promise for applications in monitoring cancer metastasis, bio-detection, and beyond.

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Acoustofluidic precise manipulation: Recent advances in applications for micro/nano bioparticles

Li,

Yao,

et al. 2024

Advances in Colloid and Interface Science

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Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Abstract: We present a microstructure-enhanced microfluidic chip for efficient DNA fragmentation, suitable for next-generation sequencing platforms. The improved on-chip performance arises from acoustic streaming generated by oscillating microstructures.

Cited by 6 publications

References 65 publications

Acoustic Streaming Efficiency in a Microfluidic Biosensor with an Integrated CMUT

Acoustic Streaming Efficiency in a Microfluidic Biosensor with an Integrated CMUT

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Acoustofluidic precise manipulation: Recent advances in applications for micro/nano bioparticles

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