Polydopamine-based molecular imprinting on silica-modified magnetic nanoparticles for recognition and separation of bovine hemoglobin

Jia, Xiaoping; Xu, Minli; Wang, Yuzhi; Ran, Dan; Shan, Yang; Zhang, Min

doi:10.1039/c2an36313e

Cited by 171 publications

(78 citation statements)

References 39 publications

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“…In this work, the MIP1 exhibited significant adsorption selectivity for BHb with IF of 4.23 and SC for other proteins being higher than 2.40 (Supporting Information Table S2). Compared with some studies, the IF in this work (IF = 4.23) was higher [33,34]. These results further proved the excellent imprinting efficiency of the present method.…”

Section: Adsorption Specificitysupporting

confidence: 70%

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Gao

Zhang

Hao

et al. 2015

J of Separation Science

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A novel magnetic core-shell polydopamine-cupric ion complex imprinted polymer was prepared in one-step through surface imprinting technology, which could specifically recognize bovine hemoglobin from the real blood samples. The polymerization conditions and adsorption performance of the resultant nanomaterials were investigated in detail. The results showed that the cupric ion played an important role in the recognition of template proteins. The saturating adsorption capacity of this kind of imprinted polymers was 2.23 times greater than those of imprinted polymers without cupric ion. The imprinting factor of the imprinted materials was as high as 4.23 for the template molecule. The selective separation bovine hemoglobin from the real blood sample is successfully applied. In addition, the prepared materials had excellent stability and no obvious deterioration after five adsorption-regeneration cycles. Easy preparation, rapid separation, high binding capacity and satisfactory selectivity for the template protein make this polymer attractive in the separation of high-abundance proteins.

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Section: Adsorption Specificitysupporting

confidence: 70%

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Gao

Zhang

Hao

et al. 2015

J of Separation Science

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“…Compared to the uncoated AZ31, the AHEC coating of AZ31 shows no obvious differences. This may be because the AHEC film is relatively thin with amorphous crystallinity [41]. However, in addition to the characteristic peak of magnesium alloy, the HA/AHEC/AZ31 produces a new sharp and narrow characteristic peak.…”

Section: Cell Viability Assay and Cell Morphology Examinationmentioning

confidence: 99%

Preparation and Characterization of Aminated Hydroxyethyl Cellulose-Induced Biomimetic Hydroxyapatite Coatings on the AZ31 Magnesium Alloy

Zhu

Sun

et al. 2017

Metals

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Abstract:The purpose of this work is to improve the cytocompatibility and corrosion resistance of magnesium alloy in the hope of preparing a biodegradable medical material. The aminated hydroxyethyl cellulose-induced biomimetic hydroxyapatite coating was successfully prepared on AZ31 magnesium alloy surface with a sol-gel spin coating method and biomimetic mineralization. Potentiodynamic polarization tests and electrochemical impedance spectroscopy showed that the hydroxyapatite/aminated hydroxyethyl cellulose (HA/AHEC) coating can greatly improve the corrosion resistance of AZ31 magnesium alloy and reduce the degradation speed in simulated body fluid (SBF). The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide] method and cell morphology observation results showed that the HA/AHEC coating on AZ31 magnesium alloy has excellent cytocompatibility and biological activity.

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“…Finally, PDA thin films were coated onto the functional Fe3O4@Au NPs by a self polymerization process. 34,35 Therefore, the carboxyl groups, amino-group and benzene-diol, have the effect of orientation imprinting on the template protein.…”

Section: Construction Of Electrochemical Sensormentioning

confidence: 99%

A Study of Electrochemical Sensor Based on BHb-imprinted Magnetic Nanoparticles

Huang

Wang

et al. 2017

ANAL. SCI.

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A study of an electrochemical sensor based on bovine hemoglobin (BHb) imprinted magnetic nanoparticles (MIPs) was investigated. First, BHb MIPs were successfully synthesized with magnetic Fe3O4@Au nanoparticles as carrier by surface modification of mercapto propionic acid for introducing carboxyl groups, combined with dopamine as the functional monomer and BHb as the template protein. Then, the MIPs were modified to the surface of a glassy carbon electrode (GCE) for electrochemical analysis by using cyclic voltammetry in the potassium ferricyanide solution. Results show that there was a good dynamic response between electrochemical signals and the adsorption amount of protein in the range of 0.05 to 0.5 mg mL -1 . Results show that the sensor exhibits a significant specific recognition toward the template protein via selective test and can be used for analysis of serum samples. The synthesized MIPs are suitable for the removal and enrichment of template protein in proteomics. At the same time, the proposed electrochemical sensor can be used for recognition of BHb.

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Polydopamine-based molecular imprinting on silica-modified magnetic nanoparticles for recognition and separation of bovine hemoglobin

Cited by 171 publications

References 39 publications

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

Preparation and Characterization of Aminated Hydroxyethyl Cellulose-Induced Biomimetic Hydroxyapatite Coatings on the AZ31 Magnesium Alloy

A Study of Electrochemical Sensor Based on BHb-imprinted Magnetic Nanoparticles

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