Polyelectrolyte Membrane Nanocoatings Aimed at Personal Protective and Medical Equipment Surfaces to Reduce Coronavirus Spreading

Grzeczkowicz, Anna; Lipko, Agata; Kwiatkowska, A.; Strawski, Marcin; Bącal, Paweł; Więckowska, Agnieszka; Granicka, Ludomira

doi:10.3390/membranes12100946

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…The selected polyelectrolytes were examined as the base of materials for cooperation with NPs for systems aimed for medical devices to maintain human cells' function. The effect of PEI and PLL, which exhibit adhesive properties towards viruses [42], on bacterial strains of S. aureus and E. coli was examined.…”

Section: The Polyelectrolyte Layer Coatings' Bacteriostatic Effect Ev...mentioning

confidence: 99%

Polyelectrolyte Platforms with Copper Nanoparticles as a Multifunctional System Aimed at Healing Process Support

Lipko,

Grzeczkowicz,

Antosiak-Iwańska

et al. 2024

Processes

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(1) Purpose: The aim of the study was to develop a nanocomposite with copper nanoparticles constituting a bacteriostatic surface to maintain human lung cell function. (2) Methods: A polyelectrolyte layer coating that incorporated copper nanoparticles was designed. As a bacteriostatic factor, copper nanoparticles were applied as a colloidal solution of copper nanoparticles (ColloidCuNPs) and a solution of copper nanoparticles (CuNPs). The influence of the polyelectrolytes on selected Gram (+) and Gram (−) strains was examined. The function and morphology of the human adenocarcinoma A549 cell line, comprising human epithelial lung cells cultured in the presence of nanocomposite layer coatings, were evaluated. We applied fluorescence and scanning electron microscopies, as well as flow cytometry, for these studies. Furthermore, the layer coating material was characterized by atomic force microscopy (AFM) and energy dispersive X-ray analysis (EDX). (3) Results: It was observed that the polyelectrolytes polyethyleneimine (PEI) and poly-L-lysine (PLL) did not induce proliferation of the E. coli strain. However, they did induce the proliferation of the S. aureus strain. Due to the effectiveness of the CuNPs against the E. coli strain, CuNPs were selected for further research. The designed coatings of proper NPs shared the sustained function of human lung cells within 10 days of culture. The AFM and EDX characterization confirmed the presence of copper in the layer coating nanomaterial. The presence of CuNPs in polyethyleneimine-based nanocomposite deepened the bacteriostatic effect on E. coli compared with PEI alone. Meanwhile, incorporating CuNPs in PLL allowed A549 cell maintenance but did not exert a bacteriostatic influence on the examined strain. (4) Conclusions: The platform based on polyelectrolytes, incorporated with copper nanoparticles, that ensures the growth and appropriate morphology of the human lung epithelial cells, might be considered an element of a system for medical devices used to maintain the function of human lung cells.

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Section: The Polyelectrolyte Layer Coatings' Bacteriostatic Effect Ev...mentioning

confidence: 99%

Polyelectrolyte Platforms with Copper Nanoparticles as a Multifunctional System Aimed at Healing Process Support

Lipko,

Grzeczkowicz,

Antosiak-Iwańska

et al. 2024

Processes

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“…The materials used in the fight against SARS-CoV-2 may include polymers applied in various ways [ 91 , 92 ]. Polymers can act as anti-viral factors interacting with the surface of the virus.…”

Section: Anti-viral Surfaces and Coatings And Their Mechanisms Of Actionmentioning

confidence: 99%

“…Experimental results indicate that the balance between ionic and hydrophobic interactions plays a leading role not only in the construction of self-assembled systems but also in the functional properties of the bioactive interface [ 95 ]. Therefore, building different multilayers based on hydrogen bonding can be introduced as a mild process, allowing the maintenance of the function of cells that come into contact with the layer [ 96 , 97 , 98 , 99 ], and, on the other hand, adsorbing viruses [ 92 ]. The hydrogen-bonded PE multilayer involves hydrogen interactions of the polymers using non-ionic layer-by-layer complexation.…”

Section: Anti-viral Surfaces and Coatings And Their Mechanisms Of Actionmentioning

confidence: 99%

Anti-Viral Surfaces in the Fight against the Spread of Coronaviruses

Kwiatkowska

Granicka

2023

Membranes

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This review is conducted against the background of nanotechnology, which provides us with a chance to effectively combat the spread of coronaviruses, and which primarily concerns polyelectrolytes and their usability for obtaining protective function against viruses and as carriers for anti-viral agents, vaccine adjuvants, and, in particular, direct anti-viral activity. This review covers nanomembranes in the form of nano-coatings or nanoparticles built of natural or synthetic polyelectrolytes––either alone or else as nanocomposites for creating an interface with viruses. There are not a wide variety of polyelectrolytes with direct activity against SARS-CoV-2, but materials that are effective in virucidal evaluations against HIV, SARS-CoV, and MERS-CoV are taken into account as potentially active against SARS-CoV-2. Developing new approaches to materials as interfaces with viruses will continue to be relevant in the future.

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“…Some attention has been paid to wound dressing, which plays a vital role in post-operative aftercare, and it is necessary to develop dressings for applications on the border of soft and hard tissues. Grzeczkowicz et al [ 5 ] studied multifunctional polyelectrolyte-layered membrane nanocomposites enhanced, among others, by hydroxyapatite nanoparticles and gold nanoparticles, and/or fullerenol to achieve a wound dressing that could be applied on the bone–skin interface. The obtained results indicate that the designed bilayer membranes prevent the internalization of bacteriostatic elements caused by human osteoblastic cells, simultaneously ensuring the proper morphology of the cells maintained within.…”

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confidence: 99%