Polyelectrolyte multilayer capsules with quantum dots for biomedical applications

Adamczak, M.; Hoel, Hanna Julie; Gaudernack, Gustav; Barbasz, Jakub; Szczepanowicz, Krzysztof; Warszyński, Piotr

doi:10.1016/j.colsurfb.2011.10.028

Cited by 33 publications

(19 citation statements)

References 38 publications

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“…In the present work nanocapsules with the core-shell structure were synthesized using nanoemulsification and the layer-by-layer (LbL) saturation method analogous to the method described previously [11,16,[32][33][34][35][36][44][45][46]. Emulsion cores were formed by addition of oil phase to polycation solution during mixing with the magnetic stirrer at 300 rpm.…”

Section: Preparation Of Nanocapsulesmentioning

confidence: 99%

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Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Piotrowski

Jantas

Szczepanowicz

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Preparation Of Nanocapsulesmentioning

confidence: 99%

“…Nanocapsules were synthesized using the method described by Szczepanowicz et al [31] and repeatedly cited in further papers [11,16,[32][33][34][35][36]. Briefly, nanocapsules' synthesis was based on direct encapsulation of emulsion cores in (bio)polyelectrolyte multilayer shells.…”

Section: Nanocapsules' Synthesismentioning

confidence: 99%

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Piotrowski

Jantas

Szczepanowicz

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…(1) Bead identification accuracies as high as 99.99% (a) CdSe/ZnS [51] (2) The coding and target signals of DNA hybridization at the single-bead level QDs are absorbed into the subsurface region of microbeads, as the solvent is removed (1) Both single-and dual-color codes were also obtained (b) CdSeTe [52] (2) Good detection sensitivity and low cytotoxicity on suspension immunoassay for goat antimouse IgG on the xMAP platform QDs are electrostatically bound to the surfaces of the microspheres, using layer-by-layer strategy (a) CdTe [53] More flexibility for creating QD-beads in biomedicine applications (sensing, immunoassay, encoding, and diagnostic) (b) CdTe [54] Cytotoxicity reductions QDs grow around preformed silica spheres CdSe, CdSe/ZnS, or CdSe/ZnSe/ZnS [55] A comparatively stable and noncytotoxic intracellular delivery already addressed many problems related to undesired ligand exchange reactions.…”

Section: Approachesmentioning

confidence: 99%

Semiconductor Quantum Dots Surface Modification for Potential Cancer Diagnostic and Therapeutic Applications

Wang

Han

et al. 2012

Journal of Nanomaterials

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Semiconductor Quantum dots (QDs) have generated extensive interest for biological and clinical applications. These applications arise from their unique properties, such as high brightness, long-term stability, simultaneous detection of multiple signals, tunable emission spectra. However, high-quality QDs, whether single or core-shell QDs, are most commonly synthesized in organic solution and surface-stabilized with hydrophobic organic ligands and thus lack intrinsic aqueous solubility. For biological applications, very often it is necessary to make the QDs dispersible in water and therefore to modify the QD surfaces with various bifunctional surface ligands or caps to promote solubility in aqueous media. Well-defined methods have been developed for QD surface modification to impart biocompatibility to these systems. In this review, we summarize the recent progress and strategies of QDs surface modification for potential cancer diagnostic and therapeutic applications. In addition, the question that arose from QD surface modification, such as impact of size increase of QD bioconjugates after surface-functionalization or surface modification on photophysical properties of QDs, are also discussed.

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“…The most common methods are surface functionalization strategies through the linkage of hydrophilic molecules on the surface of QDs or encapsulation of QDs in water-soluble materials [ 15 – 17 ]. Commonly used hydrophilic molecules include amphiphilic polymers, amphiphilic lipid molecules, and hydrophilic groups (e.g., alcoholic, sulfhydryl, and carboxyl groups) [ 18 – 21 ]. Water solubility and stability of QDs are increased, and bio-toxicity is reduced through the covalent modification of QDs surfaces [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling

et al. 2015

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Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

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Polyelectrolyte multilayer capsules with quantum dots for biomedical applications

Cited by 33 publications

References 38 publications

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Polyelectrolyte-coated nanocapsules containing undecylenic acid: Synthesis, biocompatibility and neuroprotective properties

Semiconductor Quantum Dots Surface Modification for Potential Cancer Diagnostic and Therapeutic Applications

Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling

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