Polyethyleneimine-AuNPs-copper protoporphyrin nanocomposite: a novel biosensor for sensitive detection of hydrogen peroxide in human serum

Liu, Yanju; Guo, Zhuangzhuang; Hao, Lulu; Shi, Xin-Chang; Yan, Shuxun; Yang, Huai-Xia

doi:10.1007/s10008-019-04322-5

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…Similarly, when gold nanoparticles (GNPs) of different particle sizes and plasmonic properties were synthesized by chemical reduction on the chip, PEI could rapidly adsorb them. This method significantly increased the MetaSPR biosensor platform's sensitivity because of the GNPs' potent surface plasmon absorption and excellent light-scattering qualities [37][38][39].…”

Section: Resultsmentioning

confidence: 99%

Rapid, Multispecies Detection of SARS-CoV-2 Antibodies via a Meta-Surface Plasmon Resonance Biosensor

Zhao,

Li,

Liu

et al. 2024

Transboundary and Emerging Diseases

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Public health concerns have been raised by numerous reports of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and its variations infecting a range of animals. Wildlife reservoirs may facilitate the evolution of viral types capable of causing human infection in the future. Therefore, epidemiological monitoring of animals in close contact with humans is necessary. Yet, infection symptoms are not obvious in most animals, which leads to a short nucleic acid test-detection period and limits the application of this method in animals. The use of virus- and pseudovirus-based neutralizing antibody detection techniques is restricted to establishments with elevated biosafety standards. Traditional enzyme-linked immunosorbent assays (ELISA) do not offer multispecies detection and are time-consuming and labor-intensive. This work developed a polyethyleneimine-gold nanoparticle meta-surface plasmon resonance biosensor system-based multispecies SARS-CoV-2 antibody detection platform that is fast, sensitive, has a high throughput, and is fully automated. The test can be done in 30 min and specificity is up to 100% for detection in cats, dogs, and minks. Moreover, the coincidence rate was up to 99.36% (313/315) for the detection of pseudovirus in clinical and immune sera. Additionally, this method’s detection sensitivity in cat, dog, and mink serum is 2,048, 1,024, and 4,096 times, which is much better than indirect ELISA and comparable to indirect immunofluorescence assays. An efficient method for COVID-19 epidemiology screening in animal serum will be made available by this platform.

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Section: Resultsmentioning

confidence: 99%