Polygalacturonases, polygalacturonase-inhibiting proteins and pectic oligomers in plant–pathogen interactions

D’Ovidio, R.; Mattei, Benedetta; Roberti, Serena; Bellincampi, Daniela

doi:10.1016/j.bbapap.2003.08.012

Cited by 189 publications

(131 citation statements)

References 80 publications

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“…The initial mechanism of invasion for fungus is to attack plant using CwDes such as cellulases, polygalacturonases, xylanases, and proteinases, etc. These cell wall fragments act as elicitors which elicit defense reactions in host including the production of ROS and the secretion of antifungal proteins (D'Ovidio et al 2004), such as PR proteins and inhibitor proteins (PGIP, XIP, etc.). Apart from CwDes, recent studies have revealed various effector proteins and signal transduction components like mitogen-activated protein (MAP) kinases that play a possible role in the disease-causing mechanism.…”

Section: Fungal Defensementioning

confidence: 99%

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Swarupa

Ravishankar

Rekha

2014

Planta

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Section: Fungal Defensementioning

confidence: 99%

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Swarupa

Ravishankar

Rekha

2014

Planta

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“…Among these molecules, enzymes that degrade the cell wall (cell wall-degrading enzymes, CWDEs) represent an important pathogenicity factor, and endopolygalacturonases (PGs) are one of the first CWDEs released during the infection process (Kalunke et al, 2011). Host plants produce PGIPs that recognize PGs, and prevent enzymatic action during invasion and the release of nutrients required for the growth of the pathogen (D'Ovidio et al, 2004a). Besides the inhibition of PGs, the interaction between PGs and PGIPs promotes the formation of oligogalacturonides, which are elicitors of a variety of defense responses (Cervone et al, 1989;Ridley et al, 2001;Ferrari et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)

et al. 2016

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ABSTRACT. The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different common bean (Phaseolus vulgaris L.) genotypes and its relationship with resistance to white mold (Sclerotinia sclerotiorum); ii) determine the expression levels of PvPGIP genes at different time points after inoculation with white mold; and iii) investigate differences in PvPGIP gene expression between two white mold isolates with different levels of aggressiveness. Four bean lines were analyzed, including two lines from a recurrent selection for white mold (50/5 and 84/6), one resistant line that was not adapted to Brazilian conditions (Cornell 605), and one susceptible line (Corujinha). Gene expression was investigated at 0, 1, 2, 3, and 5 days after inoculation. The isolate UFLA 03 caused no significant difference in the relative expression of any gene examined, and was inefficient in discriminating among the genotypes. For the isolate UFLA 116, all of the genes were differentially expressed, as they were associated with resistance to white mold, and the expressions increased until the third day after inoculation. The 50/5 line was not significantly different from the Corujinha line for all of the genes analyzed. However, this line had a resistance level that was similar to that of Cornell 605, according to the straw test. Therefore, the incorporation of PvPGIP genes can increase the resistance of lines derived from recurrent selection.

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“…The degradation of pectin by PGs leads to the release of oligogalacturonides (OGAs), which may activate a variety of defense responses (Prade et al, 1999;D'Ovidio et al, 2004). OGAs act as damage-associated molecular patterns (DAMPs) via their perception by the cell wallassociated receptor Wall-Associated Kinase1 (WAK1; Brutus et al, 2010).…”

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confidence: 99%

Fungal Endopolygalacturonases Are Recognized as Microbe-Associated Molecular Patterns by the Arabidopsis Receptor-Like Protein RESPONSIVENESS TO BOTRYTIS POLYGALACTURONASES1

et al. 2013

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Plants perceive microbial invaders using pattern recognition receptors that recognize microbe-associated molecular patterns. In this study, we identified RESPONSIVENESS TO BOTRYTIS POLYGALACTURONASES1 (RBPG1), an Arabidopsis (Arabidopsis thaliana) leucine-rich repeat receptor-like protein, AtRLP42, that recognizes fungal endopolygalacturonases (PGs) and acts as a novel microbe-associated molecular pattern receptor. RBPG1 recognizes several PGs from the plant pathogen Botrytis cinerea as well as one from the saprotroph Aspergillus niger. Infiltration of B. cinerea PGs into Arabidopsis accession Columbia induced a necrotic response, whereas accession Brno (Br-0) showed no symptoms. A map-based cloning strategy, combined with comparative and functional genomics, led to the identification of the Columbia RBPG1 gene and showed that this gene is essential for the responsiveness of Arabidopsis to the PGs. Transformation of RBPG1 into accession Br-0 resulted in a gain of PG responsiveness. Transgenic Br-0 plants expressing RBPG1 were equally susceptible as the recipient Br-0 to the necrotroph B. cinerea and to the biotroph Hyaloperonospora arabidopsidis. Pretreating leaves of the transgenic plants with a PG resulted in increased resistance to H. arabidopsidis. Coimmunoprecipitation experiments demonstrated that RBPG1 and PG form a complex in Nicotiana benthamiana, which also involves the Arabidopsis leucine-rich repeat receptor-like protein SOBIR1 (for SUPPRESSOR OF BIR1). sobir1 mutant plants did not induce necrosis in response to PGs and were compromised in PG-induced resistance to H. arabidopsidis.

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Polygalacturonases, polygalacturonase-inhibiting proteins and pectic oligomers in plant–pathogen interactions

Cited by 189 publications

References 80 publications

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)

Fungal Endopolygalacturonases Are Recognized as Microbe-Associated Molecular Patterns by the Arabidopsis Receptor-Like Protein RESPONSIVENESS TO BOTRYTIS POLYGALACTURONASES1

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