Polyglycosylceramides with branched N-acetyllactosamine sequences are synthesized by the human pancreatic carcinoma cell line PANC-1.

Polyglycosylceramides, Poly-N-acetyllactosamine-Containing Glycosphingolipids: Methods of Analysis, Structure, and Presumable Biological Functions

“…Polyglycosylceramides were isolated also from pancreatic carcinoma cell line PANC-1 using a combination of detergent solubilization, protease digestion, and reverse-phase chromatography . The glycolipids were degraded by ceramide glycanase, and the released saccharides were analyzed by methylation and degradation tests and by lectin binding.…”

“…During this procedure a significant part of the material was lost due to the incomplete separation from proteins and incomplete elution from the gels. Other preparative methods included extraction with other mixtures of solvents, 21 use of detergents, 15,21 protease digestion, 22 reverse-phase chromatography, 22 and peracetylation. 23 Some of these approaches, e.g., use of detergents during column separations 21 or peracetylation of crude fractions, 23 allowed for better yields during isolation procedures.…”

“…Polyglycosylceramides were isolated also from pancreatic carcinoma cell line PANC-1 using a combination of detergent solubilization, protease digestion, and reverse-phase chromatography. 22 The glycolipids were degraded by ceramide glycanase, and the released saccharides were analyzed by methylation and degradation tests and by lectin binding. The structures reported for these PGCs were in agreement with a linear chain with short side branches (model A in Figure 2), with 16-18 monosaccharides in polylactosamine core chains and 1-2 mol of sialic acid in R2-3 linkage to penultimate galactose.…”

In VitroBiosynthesis of a Decasaccharide Prototype of Multiply Branched Polylactosaminoglycan Backbones

“…Multiply branched polylactosaminoglycan backbones are present for example in glycoproteins of human red blood cells (Fukuda et al, 1984b), in murine (Muramatsu et al, 1978) and human embryonal carcinoma cells (Rasilo & Renkonen, 1982;Fukuda et al, 1985), as well as in the parasitic protozoan Trypanosoma brucei (Zamse et al, 1991). They are also expressed in glycolipids from several types of human and animal cells (Gardas, 1976;Koscielak et al, 1976;Dabrowski et al, 1984;Dabrowski et al, 1988;Hanfland et al, 1988;Nudelman et al, 1989;Barnett & Clark, 1992). Additional examples are likely to be found as experimental recognition of these structures develops further.…”

“…Polylactosaminoglycans consist of repeating N -acetyllactosamine (LacNAc) units linked together by β(1−3‘) linkages to linear backbone chains. In branched polylactosaminoglycans, some of the 3-substituted galactose residues within the primary chains are substituted also at position 6 by additional backbone elements.…”