Polygoni Multiflori Radix Preparat Delays Skin Aging by Inducing Mitophagy

Abstract

Background. As the skin is the largest organ of the human body, it is aging inevitably and produces cosmetic and psychological problems, and even disease. Therefore, the molecular mechanisms related to the prevention of skin aging need to be further explored. Methods. Aging models were constructed by D-galactose. Mice were administrated with polygoni multiflori radix preparat (PMRP), PMRP and 3-methyladenine, or PMRP and rapamycin intragastrically. The apparent and viscera index of aged rats was measured. Then, the physicochemical property, antioxidant ability, histological structure, mitochondrial membrane potential, ATP and ROS levels, and mitophagy of aged skins were determined. Finally, the expression of PINK1, Parkin, P62, and LC3II/I; apoptosis-related proteins; and the percentage of apoptotic cells were measured. Results. PMRP relieved skin aging with reducing of thymus index, improvement of pathological damage and histological structure, increase of the expression area of fibrous tissue, the ratio of type I to type III collagen, and antioxidant ability of aged skins. Importantly, PMRP also improved mitochondrial dysfunction with an increase in the content of mitochondrial membrane potential and ATP and a decrease of ROS levels. Moreover, mitophagy was enhanced with the treatment of PMRP when observed using electron microscopy, and the expression of PINK1, Parkin, and LC3I/II was increased with PMRP treatment but P62 expression was decreased. Meanwhile, PMRP alleviated apoptosis with a decrease of apoptotic cell and the expression of Cleaved-cas3, Bax, Cyt-c, AIF, and Smac as well as an increase of Bcl-2 expression. Conclusion. The results demonstrated that the polygoni multiflori radix preparata may delay skin aging by inducing mitophagy.

Ablation of Shank3 alleviates cardiac dysfunction in aging mice by promoting CaMKII activation and Parkin-mediated mitophagy

“…Dysregulated mitophagy is commonly seen in cardiovascular pathologies, including cardiac hypertrophy, heart failure and dilated cardiomyopathy [ 12 , 13 ]. Downregulation of mitophagy has been reported to contribute to the organ aging process [ [14] , [15] , [16] ]. Notably, defective mitophagy is commonly observed in aging cardiomyocytes, which leads to the accumulation of defective mitochondria.…”

Pharmacokinetics, Tissue Distribution, and Excretion Characteristics of a Radix Polygoni Multiflori Extract in Rats

“…Moldenke (Polygonaceae), is a popular traditional Chinese medicine that has been used for hair darkening, prolonging life, and mitigating several dysfunctions ( Tang et al, 2017 ). Modern pharmacological research and clinical practices have shown that RPM has several bioactive properties, including antioxidant ( Liu X. et al, 2021 ; Wang et al, 2008 ), antiaging ( Cheung et al, 2014 ; Fan et al, 2021 ), and antitumor activities ( Ma et al, 2012 ; Hou et al, 2013 ; Way et al, 2014 ; Lin et al, 2017 ; Xing et al, 2019 ), and it helps in regulating immunity ( Chen et al, 2012 ), lowering blood lipids ( Yao et al, 2013 ; Chang et al, 2016 ; Xie et al, 2012 ), and promoting neuroprotection ( Sun et al, 2011 ; Yang X.-P. et al, 2014 ; Ruan et al, 2021 ). However, an increasing number of recently published studies have demonstrated the adverse effects of RPM.…”

Preparation and Safety Evaluation of Centella asiatica Total Glycosides Nitric Oxide Gel and Its Therapeutic Effect on Diabetic Cutaneous Ulcers

“…e reproductive system is susceptible to the influence of toxicants [41], so research on reproductive toxicity is indispensable. Viscera index is an objective, practical, and sensitive indicator in toxicology, which can directly reflect the comprehensive toxicity of viscera after medication and is an essential clue to the target organ of toxicant action; e decrease or increase of viscera index can reflect the atrophy or congestion of viscera [42].…”