Polyhydroxyalkanoate production from crude glycerol by newly isolated Pandoraea sp.

Paula, Fabrício Coutinho de; Kakazu, Sérgio; Paula, Carolina Bilia Chimello de; Gomez, José Gregório Cabrera; Contiero, Jonas

doi:10.1016/j.jksus.2016.07.002

Cited by 65 publications

(38 citation statements)

References 31 publications

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“…Besides, the recovery yield is superior to that obtained to Yezza et al, [35] who reported that, after 66 h, a copolymer content of 30 % (w/w) was achieved when 1 % (v/v) methanol solution supplemented with 0.1 % (v/v) valeric acid used as substrate. In addition, the yield (Y p/s ) of 0.228 g/g obtained in this study is about 84.5 % of the maximum theoretical yield based on glycerol (0.47 g/g) via acetyl-CoA pathway [22].…”

Section: Discussionsupporting

confidence: 47%

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

2017

J App Biol Biotech

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Polyhydroxyalkalonates (PHAs) are biodegradable thermoplastics that are receiving immense attention as an alternative to petroleum derived plastics. The present study aimed to produce PHA using cheap and ecofriendly biodiesel waste glycerol as a substrate. Several PHA-producing bacterial isolates were isolated from different environmental samples and their efficacy for PHA production was assessed. Isolate Azu-IN1 showed the highest PHA production and was identified as Zobellella taiwanensis Azu-IN1 using 16S rRNA gene sequence and biochemical characterization. Factors affecting PHA production were optimized in batch fermentations. Supplementation of ammonium chloride as nitrogen source, methanol as an auxiliary carbon source, and agitation rate are the main limiting factors affecting PHA production. Maximum PHA production of 2.65 g/L at recovery yield of 50.3 (%), w/w) was achieved after 36 h in batch fermentation using optimized medium. Enhanced PHA production of 3.73 g/L with recovery yield of 61.7 % (w/w) was obtained in fed batch fermentation. The characteristics of extracted PHA were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) 1 H and 13 C Nuclear Magnetic Resonance (NMR) spectroscopy. This is the first report on accumulation of PHA by Zobellella taiwanensis using glycerol as the sole carbon source.

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Section: Discussionsupporting

confidence: 47%

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

2017

J App Biol Biotech

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“…This value is similar to the 3HV yield obtained from pure glycerol by this mutant strain of 0.78 g g −1 and reveals a significant improvement of the conversion rates of 3HV monomers from PA compared to the wild type strain Pandoraea sp. MA03 which showed a maximum yield of 0.19 gg −1 in previous experiments . Furthermore, cultivations in MSM containing CG and PA showed a maximum polymer production and accumulation of 2.5 g L −1 and 62.3% CDW, respectively, with 3HV fractions varying from 21.9 to 30 mol% (Figures A,B).…”

Section: Resultsmentioning

confidence: 58%

“…MA03 which showed a maximum yield of 0.19 gg 21 in previous experiments. 22 Furthermore, cultivations in MSM containing CG and PA showed a maximum polymer production and accumulation of 2.5 g L 21 and 62.3% CDW, respectively, with 3HV fractions varying from 21.9 to 30 mol% (Figures 2A,2B). These results obtained from cultivations in CG are essential for the PHA production at industrial scale since the purification of this carbon source would be economically counterproductive.…”

Section: Mutant Selectionmentioning

confidence: 90%

Poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) production from biodiesel by‐product and propionic acid by mutant strains of Pandoraea sp.

Paula

Gomez

et al. 2017

Biotechnology Progress

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Pandoraea sp. MA03 wild type strain was subjected to UV mutation to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was selected from 52 mutants affected in the propionate metabolism exhibiting a conversion rate of PA into 3HV units of 0.78 g g . The use of crude glycerol (CG) plus PA or valeric acid resulted in a copolymer with 3HV contents varying from 21.9 to 30 mol% and 22.2 to 36.7 mol%, respectively. Fed-batch fermentations were performed using CG and PA and reached a 3HV yield of 1.16 g g , which is 86% of the maximum theoretical yield. Nitrogen limitation was a key parameter for polymer accumulation reaching up to 63.7% content and 18.1 mol% of 3HV. Henceforth, mutant prp25 is revealed as an additional alternative to minimize costs and support the P(3HB-co-3HV) production from biodiesel by-products. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1077-1084, 2017.

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“…MA03 achieved a considerable amount of intracellular biopolymer (63.6% cell dry weight [CDW]) and final PHB production was 2.9 g L −1 from 2% crude glycerol after 72 hours of cultivation. 77 Zobellella denitrificans utilized crude glycerol (2%) to produce PHA with yield up to 2.82 g L −1 , and those were around fourfold that compared with the amount of PHA produced by Bacillus megaterium INCQS 425 and Cupriavidus necator IPT 027 of 0.78 and 0.33 g L −1 , respectively. 86 Different concentrations of crude glycerol were used for production of PHA by Bacillus thuringiensis EGU45, whereas the accumulation of PHA inside the bacterial cells increased from 1540 to 3585 mg L −1 by raising the crude glycerol input from 1% to 5%.…”

Section: Discussionmentioning

confidence: 99%

Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

et al. 2019

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Summary This study demonstrated a biotechnological approach for simultaneous production of low‐cost H2, liquid biofuels, and polyhydroxyalkanoates (PHAs) by solventogenic bacterium (Clostridium beijerinckii) from renewable industrial wastes such as molasses and crude glycerol. C beijerinckii ASU10 (KF372577) exhibited considerable performance for hydrogen production of 5.1 ± 0.84 and 11 ± 0.44 mL H2 h−1 on glycerol and sugarcane molasses, respectively. The total acetone‐butanol‐ethanol (ABE) generation from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L−1, respectively. ABE productivity (g L−1 h−1) was 0.0486 and 0.0564 with a yield rate (g g−1) up to 0.508 and 0.493 from glycerol and molasses fermentation, respectively. The PHA yields from glycerol and sugarcane molasses were 84.37% and 37.97% of the dried bacterial biomass, respectively. Additionally, the ultrathin section of C beijerinckii ASU10 showed that PHA granules were accumulated more densely on glycerol than molasses. Gas chromatography–mass spectrometry (GC‐MS) analysis confirmed that the PHAs obtained from molasses fermentation included 3‐hydroxybutyrate (47.3%) and 3‐hydroxyoctanoate (52.7%) as the main constituents. Meanwhile, 3‐hydroxybutyrate represented the sole monomer of PHA produced from glycerol fermentation. This study demonstrated that C beijerinckii ASU10 (KF372577) is a potent strain for low‐cost PHA production depending on its high potential to produce high‐energy biofuel and other valuable compounds from utilization of organic waste materials.

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Polyhydroxyalkanoate production from crude glycerol by newly isolated Pandoraea sp.

Cited by 65 publications

References 31 publications

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

Poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) production from biodiesel by‐product and propionic acid by mutant strains of Pandoraea sp.

Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

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