Polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge by PHA-storing denitrifiers integrating PHA accumulation with nitrate removal

Tu, Weiming; Zhang, Dandan; Wang, Hui; Lin, Ziyu

doi:10.1016/j.biortech.2019.121895

Cited by 45 publications

(9 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For these reasons, the utilization of food processing by-products or wastes from water treatment systems as carbon sources for PHA production with high comonomer contents is seen as a very promising alternative . Thus, different organic wastes from both citric and dairy industries, − oils and biodiesel, , and even sludge from treatment plants − have been explored for the production of PHAs. In this context, the use of mixed microbial cultures (MMCs) particularly represents an affordable approach to reduce costs since sterile conditions and specific feedstock are not necessary .…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Electrospun Biopapers of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Derived from Cheese Whey with Varying 3-Hydroxyvalerate Contents

et al. 2021

View full text Add to dashboard Cite

In the present study, three different newly developed copolymers of poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) with 20, 40, and 60 mol % contents in 3-hydroxyvalerate (3HV) were produced by the biotechnological process of mixed microbial cultures (MMCs) using cheese whey (CW), a by-product from the dairy industry, as feedstock. The CW-derived PHBV copolyesters were first purified and then processed by solution electrospinning, yielding fibers of approximately 2 μm in cross-section in all cases. The resultant electrospun PHBV mats were, thereafter, post-processed by annealing at different temperatures, below their maximum of melting, selected according to their 3HV content in order to obtain continuous films based on coalesced fibers, so-called biopapers. The resultant PHBV films were characterized in terms of their morphology, crystallinity, and mechanical and barrier properties to assess their potential application in food packaging. The CW-derived PHBV biopapers showed high contact transparency but a slightly yellow color. The fibers of the 20 mol % 3HV copolymer were seen to contain mostly poly(3-hydroxybutyrate) (PHB) crystals, the fibers of the 40 mol % 3HV copolymer a mixture of PHB and poly(3-hydroxyvalerate) (PHV) crystals and lowest crystallinity, and the fibers of the 60 mol % 3HV sample were mostly made of PHV crystals. To understand the interfiber coalesce process undergone by the materials during annealing, the crystalline morphology was also assessed by variable-temperature both combined small-angle and wide-angle X-ray scattering synchrotron and Fourier transform infrared experiments. From these experiments and, different from previously reported biopapers with lower 3HV contents, all samples were inferred to have a surface energy reduction mechanism for interfiber coalescence during annealing, which is thought to be activated by a temperature-induced decrease in molecular order. Due to their reduced crystallinity and molecular order, the CW-derived PHBV biopapers, especially the 40 mol % 3HV sample, were found to be more ductile and tougher. In terms of barrier properties, the three copolymers performed similarly to water and limonene, but to oxygen, the 40 mol % sample showed the highest relative permeability. Overall, the materials developed, which are compatible with the Circular Bioeconomy organic recycling strategy, can have an excellent potential as barrier interlayers or coatings of application interest in food packaging.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Electrospun Biopapers of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Derived from Cheese Whey with Varying 3-Hydroxyvalerate Contents

et al. 2021

View full text Add to dashboard Cite

show abstract

“…NH 4 + –N, NO 2 – –N, NO 3 – –N, COD, and CDW were measured according to the methods reported by Gilcreas . The concentrations of VFAs and PHAs were measured using gas chromatography (GC-2014, Shimadzu, Japan) with a flame ionization detector and a DB-FFAP capillary column (25 m × 0.25 mm × 0.25 μm) based on a previous study …”

Section: Methodsmentioning

confidence: 99%

Polyhydroxyalkanoate Production from Food Waste Coupled with Aerobic Nitrate Reduction

Liu

Xiong

et al. 2021

ACS Sustainable Chem. Eng.

Self Cite

View full text Add to dashboard Cite

Polyhydroxyalkanoate (PHA) production from wastes by mixed microbial cultures has been developed into an integrated process to couple with nitrate removal due to its economic and environmental benefits. However, the anoxic conditions for denitrification result in low productivity of PHA production due to the limited energy supply. In this work, the fully aerobic feeding regime uncoupling carbon and nitrogen supply was applied to enhance the culture enrichment and productivity while being accompanied by nitrate removal. The enriched cultures showed a nitrate assimilation rate of 2.35 ± 1.10 mg of NO 3 − −N per gram of active biomass (X a ) per hour; meanwhile, the specific growth rate (μ X a , 0.019 ± 0.002 h −1 ) and biomass yield on PHA (Y X a /PHA , 0.68 ± 0.15 g X a /g PHA) were higher than those of a classic anoxic process (μ X a , 0.011 ± 0.001 h −1 and Y X a /PHA , 0.38 ± 0.09 g X a /g PHA). Furthermore, ammonia and oxygen were confirmed to have significant impacts on regulating nitrate utilization by directing nitrate flow to assimilatory nitrate use, aerobic denitrification, or anoxic denitrification. Metagenome analysis revealed that the overall function was mostly undertaken by Thauera aminoaromatica, and a nitrate metabolic model driven by PHAs was proposed. In summary, these findings widened application scenarios for PHA production coupled with nitrate removal.

show abstract

“…Different conditions of (a) pH, (b) temperature, and (c) HRT during the fermentation of bio-based streams at lab-scale (part 2): cases 99–100, 101–105, 106, 107, 108, 109–116, 117–118, 119, 120, 121, 122–131, 132–136, 137, 138, 139–147, 148–151, 152–155, 156 …”

Section: Bio-based Vfa Production Through Anaerobic Fermentationmentioning

confidence: 99%

Targeted Bio-Based Volatile Fatty Acid Production from Waste Streams through Anaerobic Fermentation: Link between Process Parameters and Operating Scale

Bruni

Foglia

Eusebi

et al. 2021

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Anaerobic processes are proven to have much more environmental and economic benefits than conventional aerobic treatment systems, offering sustainable energy and valuable biochemicals. In recent years, bio-based volatile fatty acid (VFA) production has come into prominence as more value is derived before ending up with other final products. This paper presents a critical review of the research studies on bio-based VFA production from different waste streams (i.e., industrial sludge/waste, organic fraction of municipal solid waste/food waste, municipal wastewater/sludge, combined streams) through anaerobic fermentation. Fundamentals and decisive process parameters (i.e., pH, temperature, retention time, organic loading rate) are reviewed, and their correlations with VFA yields are critically discussed based on 178 cases (156 lab- and 22 pilot-scale). The picture we provided clearly demonstrates that process parameters should be clearly defined and optimized according to the type of waste streams which may have a significant impact on downstream processes in most cases.

show abstract

Polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge by PHA-storing denitrifiers integrating PHA accumulation with nitrate removal

Cited by 45 publications

References 38 publications

Development and Characterization of Electrospun Biopapers of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Derived from Cheese Whey with Varying 3-Hydroxyvalerate Contents

Development and Characterization of Electrospun Biopapers of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Derived from Cheese Whey with Varying 3-Hydroxyvalerate Contents

Polyhydroxyalkanoate Production from Food Waste Coupled with Aerobic Nitrate Reduction

Targeted Bio-Based Volatile Fatty Acid Production from Waste Streams through Anaerobic Fermentation: Link between Process Parameters and Operating Scale

Contact Info

Product

Resources

About