Polylactide Microparticles Prepared by Double Emulsion-Evaporation

Nihant, N; Schugens, C; Grandfils, Christian; Jérôme, Robert; Teyssié, Philippe

doi:10.1006/jcis.1995.1296

Cited by 80 publications

(55 citation statements)

References 20 publications

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“…Primary emulsion stability and polymer precipitation conditions play important roles in the encapsulation efficiency and the internal structure of the particles [13]. The notably small particle size of CTAB-PLGA microspheres may be attributed to the solubility of CTAB in both oil and aqueous phases as well as the electrostatic repulsion as observed in other studies [11,14].…”

Section: Discussionmentioning

confidence: 71%

Effect of Surfactants on Plasmid DNA Stability and Release from Poly (D,L-lactide-co-glycolide) Microspheres

Doolaanea¹,

Ismail²,

Mansor³

et al. 2015

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 71%

Effect of Surfactants on Plasmid DNA Stability and Release from Poly (D,L-lactide-co-glycolide) Microspheres

Doolaanea¹,

Ismail²,

Mansor³

et al. 2015

Trop. J. Pharm Res

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“…BSA is known to have surface active properties, which are responsible for its deposition at the oil-water interface [18]. This has a strong stabilizing effect on the first emulsion [19] and leads to the formation of a matrix-like structure (Figure 6). …”

Section: Deposition Behavior During Double Emulsion Techniquementioning

confidence: 99%

Structural analysis of microparticles by confocal laser scanning microscopy

2000

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This study demonstrates the potential of confocal laser scanning microscopy (CLSM) as a characterization tool for different types of microparticles. Microparticles were prepared by various methods including complex coacervation, spray drying, double emulsion solvent evaporation technique, and ionotropic gelation. Protein drugs and particle wall polymers were covalently labeled with a fluorescent marker prior to particle preparation, while low molecular weight drugs were labeled by mixing with a fluorescent marker of similar solubility properties. As was demonstrated in several examples, CLSM allowed visualization of the polymeric particle wall composition and detection of heterogeneous polymer distribution or changes in polymer matrix composition under the influence of the drug. Furthermore, CLSM provides a method for threedimensional reconstruction and image analysis of the microparticles by imaging several coplanar sections throughout the object. In conclusion, CLSM allows the inspection of internal particle structures without prior sample destruction. It can be used to localize the encapsulated compounds and to detect special structural details of the particle wall composition.

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“…was found to be more efficient in preventing the insulin aggregation in solution. In addition, poloxamers stabilize primary emulsion and reduce protein-polymer interactions (Nilhant et al 1994). During the secondary drying phase, the removal of water molecules also contributes to the protein aggregation (Gribbon et al 1996).…”

mentioning

confidence: 99%