Polymer gene delivery vectors encapsulated in thermally sensitive bioreducible shell

Ivanova, E.; Ivanova, Nadya I.; Apostolova, Margarita D.; Turmanova, Sevdalina; Dimitrov, Ivaylo

doi:10.1016/j.bmcl.2013.05.055

Cited by 6 publications

(8 citation statements)

References 21 publications

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“…Glucose and temperature dual-responsive nanospheres were fabricated by incorporation of glucose-responsive monomer (3-acrylamidophenylboronic acid) (APBA) as reported by Du et al Biodegradability of the shell. By using biodegradable cross-linking agents such as N , N ′-bis(acryloyl) cystamine (BAC), disulfide bonds were incorporated in the network of the shell. − They can be degraded under reducing conditions or via thiol–disulfide exchange occurring in the cells. Attachment of cell recognizable targeting ligands. This was demonstrated by Dimitrov by incorporating a folate-terminated PEO-macromonomer. …”

Section: Outer Shell Membrane Formation By Means Of Seeded Heterophas...mentioning

confidence: 99%

“…Biodegradability of the shell. By using biodegradable cross-linking agents such as N , N ′-bis(acryloyl) cystamine (BAC), disulfide bonds were incorporated in the network of the shell. − They can be degraded under reducing conditions or via thiol–disulfide exchange occurring in the cells.…”

Section: Outer Shell Membrane Formation By Means Of Seeded Heterophas...mentioning

confidence: 99%

“…Seeded radical copolymerization has been successively used for encapsulation of temperature-responsive polyplexes with a biodegradable cross-linked shell. − For this purpose, first plasmid or linear DNA was compacted via complex formation with cationic diblock copolymer (PNIPAM- b -PLLys or (PNIPAM- g -PEG)- b -PLLys , (Scheme ) or via interaction of plasmid DNA with cationic worm-like micelles comprising a poly(propylene oxide) core and a PDMAEMA corona (Scheme ). The polyplex particles exhibit good colloidal stability at high temperature (up to 70 °C), allowing formation of a cross-linked shell via copolymerization of NIPAM and N , N ′-bis(acryloyl) cystamine.…”

Section: Outer Shell Membrane Formation By Means Of Seeded Heterophas...mentioning

confidence: 99%

“…(A) Non-transfected cells (negative control); (B) cells transfected with Lipofectamine 2000 containing 4 μg of pEGFP-C2 (positive control); (C) transfection of initial polyplex; and (D) after polyplex stealth coating with a biodegradable PNIPAm membrane. Reprinted from ref . Copyright 2013, with permission from Elsevier.…”

Section: Nonviral Gene Delivery By Polymer-coated Polyplexesmentioning

confidence: 99%

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Polymeric Nanoparticle Engineering: From Temperature-Responsive Polymer Mesoglobules to Gene Delivery Systems

et al. 2014

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A novel approach for the preparation of nano- and microcapsules in aqueous solutions by using thermoresponsive polymer (TRP) templates (mesoglobules) is described. The method comprised three steps: formation of mesoglobules, coating the templates by seeded radical copolymerization, followed by core dissolution and core removal upon cooling. When mesoglobule entraps biomacromolecules during the process of their formation, it makes it possible to load a controlled amount of bioactive compounds without covalent attachment. Special attention is paid to the mesoglobule dissolution upon cooling, as well as their loading efficiency. Details on the outer shell formation and the possibilities for targeting ligands incorporation and control of the shell porosity are discussed. Finally, the seeded radical copolymerization was used for covering DNA complexes with cationic copolymers bearing TRP blocks. This Review is an attempt to convince researchers of the promising perspectives for using mesoglobules as potential reservoirs, carriers, and transferring agents for biologically active substances.

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Section: Outer Shell Membrane Formation By Means Of Seeded Heterophas...mentioning

confidence: 99%

Section: Outer Shell Membrane Formation By Means Of Seeded Heterophas...mentioning

confidence: 99%

Section: Outer Shell Membrane Formation By Means Of Seeded Heterophas...mentioning

confidence: 99%

Section: Nonviral Gene Delivery By Polymer-coated Polyplexesmentioning

confidence: 99%

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Polymeric Nanoparticle Engineering: From Temperature-Responsive Polymer Mesoglobules to Gene Delivery Systems

et al. 2014

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“…One of the potential routes by which such DNA-binding modulation or switching can be attained is by using responsive or “smart” polymers capable of conformational or phase changes under the effect of varying pH and temperatures. − Haladjova et al have recently reported the preparation of mesoglobules using thermosensitive polymers as potential reservoirs, vehicles, and transferring agents for biologically active substances such as DNA etc. Thermosensitive star-shaped copolymers having branched chains were designed by Zhou et al, which can potentially serve as gene carriers with improved gene transfection efficiency .…”

Section: Introductionmentioning

confidence: 99%

Temperature- and Composition-Dependent DNA Condensation by Thermosensitive Block Copolymers

et al. 2017

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Successful intracellular delivery of genes requires an efficient carrier, as genes by themselves cannot diffuse across cell membranes. Because of the toxicity and immunogenicity of viral vectors, nonviral vectors are gaining tremendous interest in research. In this work, we have investigated the temperature-dependent DNA condensation efficiency of various compositions of a thermosensitive block copolymer viz., poly(N-isopropylacrylamide)-b-poly(2-(diethylamino)ethyl methacrylate) (PNIPA-b-PDMAEMA). Three different copolymer compositions of varying molecular weights were successfully synthesized via the RAFT polymerization technique. Steady-state fluorescence and circular dichroism (CD) spectroscopies, dynamic light scattering (DLS) and zeta potential measurements, agarose gel electrophoresis, and atomic force microscopy techniques were utilized to study the interaction of the copolymers with DNA at temperatures above and below the critical aggregation temperature (CAT). All these experiments revealed that, above the CAT, there was formation of highly stable and tight polymer–DNA complexes (polyplexes). The size of polyplexes was dependent on the temperature up to a certain charge ratio, as determined by the DLS results. The results obtained from temperature-dependent fluorescence spectroscopy, CD, and gel electrophoresis indicated that the DNA molecules were shielded more from aqueous exposure above the CAT because of the formation of relatively more compact complexes. The polyplexes also exhibited changes in the particle morphology below and above the CAT, with particles generated above CAT being more spherical in morphology. These results suggested at the possibility of modulating the complex formation by temperature modification. The present biophysical studies would provide new physical insight into the design of novel gene carriers.

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Polymeric vehicles for transport and delivery of DNA via cationic micelle template method

Haladjova

Kyulavska

Doumanov³

et al. 2017

Colloid Polym Sci

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Polymer gene delivery vectors encapsulated in thermally sensitive bioreducible shell

Cited by 6 publications

References 21 publications

Polymeric Nanoparticle Engineering: From Temperature-Responsive Polymer Mesoglobules to Gene Delivery Systems

Polymeric Nanoparticle Engineering: From Temperature-Responsive Polymer Mesoglobules to Gene Delivery Systems

Temperature- and Composition-Dependent DNA Condensation by Thermosensitive Block Copolymers

Polymeric vehicles for transport and delivery of DNA via cationic micelle template method

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