Polymer live-cell array for real-time kinetic imaging of immune cells

Zurgil, Naomi; Afrimzon, Elena; Deutsch, Assaf; Namer, Yaniv; Shafran, Yana; Sobolev, Maria; Tauber, Yishay; Ravid-Hermesh, Orit; Deutsch, Mordechai

doi:10.1016/j.biomaterials.2010.02.035

Cited by 24 publications

(22 citation statements)

References 18 publications

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“…HCI assays such as nuclei density, nuclei morphology, cell proliferation, and apoptosis were demonstrated on this platform to study drug responses against cancer cells [ 82 ]. In addition, HCI assays have been demonstrated in PDMS microwells with immune cells that were monitored in real-time to evaluate cell viability, MMP, reactive oxygen species (ROS) generation, and plasma membrane integrity for the cytotoxic effect of drugs [ 94 ]. Major limitations of the microwell systems are the potential for cross-contamination between microwells, the difficulties in dispensing multiple drugs directly into the microwells and monitoring drug responses, and the requirement of expensive and time-consuming fabrication steps.…”

Section: Miniaturized 3d Cell Culture Systems and Their Applicatiomentioning

confidence: 99%

High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures

Joshi

Lee

2015

Biosensors

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High content imaging (HCI) is a multiplexed cell staining assay developed for better understanding of complex biological functions and mechanisms of drug action, and it has become an important tool for toxicity and efficacy screening of drug candidates. Conventional HCI assays have been carried out on two-dimensional (2D) cell monolayer cultures, which in turn limit predictability of drug toxicity/efficacy in vivo; thus, there has been an urgent need to perform HCI assays on three-dimensional (3D) cell cultures. Although 3D cell cultures better mimic in vivo microenvironments of human tissues and provide an in-depth understanding of the morphological and functional features of tissues, they are also limited by having relatively low throughput and thus are not amenable to high-throughput screening (HTS). One attempt of making 3D cell culture amenable for HTS is to utilize miniaturized cell culture platforms. This review aims to highlight miniaturized 3D cell culture platforms compatible with current HCI technology.

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Section: Miniaturized 3d Cell Culture Systems and Their Applicatiomentioning

confidence: 99%

High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures

Joshi

Lee

2015

Biosensors

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“…One example of a screening application utilized a hexagonal honeycomb microwell plate previously fabricated from a Polymer Live Cell Array (PLCA) to culture 3D spheroids of MCF7 breast cancer cells and to screen for antitumor drugs [35,36]. In addition to providing separate wells where the 3D spheroids could be cultured, the hexagonal microwell plate allowed for the treatment of the 3D spheroids with two anticancer drugs.…”

Section: Microscale 3d Culture Screening Systemsmentioning

confidence: 99%

Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges

Montanez-Sauri

Beebe

Sung

2014

Cell. Mol. Life Sci.

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The increasing interest in studying cells using more in vivo-like three-dimensional (3D) microenvironments has created a need for advanced 3D screening platforms with enhanced functionalities and increased throughput. 3D screening platforms that better mimic in vivo microenvironments with enhanced throughput would provide more in-depth understanding of the complexity and heterogeneity of microenvironments. The platforms would also better predict the toxicity and efficacy of potential drugs in physiologically relevant conditions. Traditional 3D culture models (e.g. spinner flasks, gyratory rotation devices, non-adhesive surfaces, polymers) were developed to create 3D multicellular structures. However, these traditional systems require large volumes of reagents and cells, and are not compatible with high throughput screening (HTS) systems. Microscale technology offers the miniaturization of 3D cultures and allows efficient screening of various conditions. This review will discuss the development, most influential works, and current advantages and challenges of microscale culture systems for screening cells in 3D microenvironments.

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“…[102][103][104] The integration of microfluidic systems with these types of arrays can further expand their utility by enabling the modulation of microenvironments or application of stimuli. 105,106 Similar to most examples of microfluidic assays, one of the main disadvantages in these systems is the efficiency of distributing stem cells into individual wells. The number of wells satisfying the cell-occupying criteria relies on the cellseeding density and the dimensions of each microwell.…”

Section: Microwell-based Analysis Systemmentioning

confidence: 99%

Technology Advancement for Integrative Stem Cell Analyses

Jeong

Choi

Lee

2014

Tissue Engineering Part B: Reviews

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Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose-by introducing a concept of vertical and horizontal approach-that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

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Polymer live-cell array for real-time kinetic imaging of immune cells

Cited by 24 publications

References 18 publications

High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures

High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures

Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges

Technology Advancement for Integrative Stem Cell Analyses

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