Polymer microfluidic devices

Becker, Holger; Locascio, Laurie E.

doi:10.1016/s0039-9140(01)00594-x

Cited by 1,067 publications

(783 citation statements)

References 62 publications

Supporting

Mentioning

757

Contrasting

Unclassified

Order By: Relevance

“…Lab-on-a-Chip) is a key enabling technology for point-of-care diagnostics as it promises improvements over standard immunoassay formats in terms of speed, sensitivity and reagent consumption, and can potentially be highly automated [11][12][13][14][15][16][17][18][19][20] . A low-cost disposable microfluidic card could, in principle, carry out the necessary tests using a small patient sample and a scant amount of expensive reagents, (such as antibodies).…”

Section: Microfluidics (And Its Integration Into What Is Known Asmentioning

confidence: 99%

Concentration Gradient Immunoassay. 1. An Immunoassay Based on Interdiffusion and Surface Binding in a Microchannel

2007

View full text Add to dashboard Cite

We describe a novel microfluidic immunoassay method based on the diffusion of a small molecule analyte into a parallel-flowing stream containing cognate antibody. This interdiffusion results in a steady-state gradient of antibody binding site occupancy transverse to convective flow. In contrast to the diffusion immunoassay (Hatch et al. Nature Biotechnology,19:461−465 (2001)), this antibody occupancy gradient is interrogated by a sensor surface coated with a functional analog of the analyte. Antibodies with at least one unoccupied binding site may specifically bind to this functionalized surface, leading to a quantifiable change in surface coverage by the antibody. SPR imaging is used to probe the spatial distribution of antibody binding to the surface and, therefore, the outcome of the assay. We show that the pattern of antibody binding to the SPR sensing surface correlates with the concentration of a model analyte (phenytoin) in the sample stream. Using an inexpensive disposable microfluidic device, we demonstrate assays for phenytoin ranging in concentration from 75 to 1000 nM in phosphate buffer. At a total volumetric flow rate of 90 nL/sec, the assays are complete within 10 minutes. Inclusion of an additional flow stream on the side of the antibody stream opposite to that of the sample enables simultaneous calibration of the assay. This assay method is suitable for rapid quantitative detection of low-molecular weight analytes for point-of-care diagnostic instrumentation.

show abstract

Section: Microfluidics (And Its Integration Into What Is Known Asmentioning

confidence: 99%

Concentration Gradient Immunoassay. 1. An Immunoassay Based on Interdiffusion and Surface Binding in a Microchannel

2007

View full text Add to dashboard Cite

show abstract

“…The master was then placed on top of the polymer with the surface to be embossed in contact with the polymer and the hot embossing proceeded in typical fashion [10,11].…”

Section: Polymer Imprintingmentioning

confidence: 99%

Production of structures for microfluidics using polymer imprint techniques

Mills¹,

Martínez²,

Bessueille³

et al. 2005

Microelectronic Engineering

View full text Add to dashboard Cite

“…1 Author to whom any correspondence should be addressed. manufacturing [11]. As well as being low-cost and disposable, polymers are highly adaptable for diagnostic purposes [12].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

Tothill

Partridge

James

et al. 2017

J. Micromech. Microeng.

View full text Add to dashboard Cite

A 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay.

show abstract

Polymer microfluidic devices

Cited by 1,067 publications

References 62 publications

Concentration Gradient Immunoassay. 1. An Immunoassay Based on Interdiffusion and Surface Binding in a Microchannel

Concentration Gradient Immunoassay. 1. An Immunoassay Based on Interdiffusion and Surface Binding in a Microchannel

Production of structures for microfluidics using polymer imprint techniques

Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

Contact Info

Product

Resources

About