Polymer-Paraburkholderia phytofirmans PsJN Coated Diammonium Phosphate Enhanced Microbial Survival, Phosphorous Use Efficiency, and Production of Wheat

Abstract

Low bioavailability of phosphorus (P) in alkaline/calcareous soils leads to low yields due to its precipitation/fixation with calcium (Ca). An effective method to enhance phosphorus use efficiency (PUE) and its availability to plants is the application of alginate bioaugmented coated di-ammonium phosphate (DAP). In this study, the pre-isolated P solubilizing Paraburkholderia phytofirmans (PsJN) coated with different concentrations of polymer (alginate) on DAP with and without carbon source was tested in incubation, pot, and field experiments to investigate microbial survival, Olsen P release, and its availability to plants. Results indicated that the maximum recovery of PsJN loaded on alginate at 30 days incubation with and without carbon source was 199 × 1010 and 82 × 106 CFU mL−1 respectively. Application of alginate bioaugmented DAP released 962 mg kg−1 Olsen P after 60 days of incubation compared to 280 and 370 mg kg−1 Olsen P released by uncoated and simple alginate coated DAP, respectively. Results from pot trial revealed that application of 100% alginate bioaugmented DAP increased 26% plant height, 31% photosynthetic rate, 55% grain yield, and 6 × 105 CFU g−1 root bacterial population, compared to uncoated DAP. Field trial results indicated that 22% grain yield, 14% straw yield, and 44% agronomy P efficiency were increased by applying 100% alginate bioaugmented DAP compared to uncoated DAP. This new approach resulted in controlled release of P from coated DAP that not only reduced phosphorus fixation but also enhanced the bioavailability of P to plants.

Deciphering the Potential of Bioactivated Rock Phosphate and Di-Ammonium Phosphate on Agronomic Performance, Nutritional Quality and Productivity of Wheat (Triticum aestivum L.)

“…Moreover, as the P sources in the present study were bioaugmented with P-solubilizing microbes, these microbes might have enhanced indigenous and applied P solubilization through diverse mechanisms such as rhizospheric acidification and nutrient solubilization which in turn have improved plant growth and yield contributing factors in the present study. These results are in accordance with previously reported works [7,86].…”

“…Furthermore, in the present study, we found higher nutritional quality parameters of wheat grains under the combined application of P sources. This could be attributed to the presence of ammonium in DAP fertilizer which on hydrolysis might have caused acidification and resultantly enhanced solubility of bound P from Ca-P complexes [7]. The increased grain quality parameters in the present study may also be due to the enhanced supply of micronutrients such as Fe, Zn, Cu and Mn to wheat crop [98,99].…”

“…It is vital to improve P availability and efficiency for better yield and improved quality of wheat grain [7,50]. The solubility of RP can be improved through its composting, by using phosphate solubilizing microbes along with organic manures [51][52][53].…”

“…Its deficiency causes stunted plant growth and ultimately severe yield losses [6]. The fertility status of Pakistani soils is following a drastic decreasing trend due to intensive, extensive, and exhaustive cultivation of crops and nutrient mining [7]. Worldwide, almost 70% of soils (acidic or alkaline) are deficient in P [8].…”

The Recovery of Phosphate and Ammonium from Biogas Slurry as Value-Added Fertilizer by Biochar and Struvite Co-Precipitation

“…Therefore, a large amount of P recovery in struvite is particularly value added [4,13,14]. Additionally, struvite is also a comprehensive nutrient fertilizer and, as such, can promote the germination and growth of vegetables [15][16][17]. At present, commonly used nitrogen and phosphorus fertilizers, such as diammonium phosphate (46% P 2 O 5 , 18% N) and triple superphosphate (48% P 2 O 5 ), have similar nitrogen and phosphorus contents to struvite [13,18,19], which suggests that struvite could potentially be marketed as a fertilizer.…”