Polymer Skulls With Integrated Transparent Electrode Arrays for Cortex‐Wide Opto‐Electrophysiological Recordings

Donaldson, Preston D.; Navabi, Zahra S.; Carter, Russell E.; Fausner, Skylar M. L.; Ghanbari, Leila; Ebner, Timothy J.; Swisher, Sarah L.; Kodandaramaiah, Suhasa B.

doi:10.1002/adhm.202200626

Cited by 19 publications

(22 citation statements)

References 77 publications

(121 reference statements)

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“…In another example, Donaldson et al demonstrated transparent PEDOT:PSS MEA for simultaneous electrical and optical recording of cortical dynamics from multiple brain regions. 122 The patterning and fabrication of the MEA relied on inkjet printing of Ag interconnects and bond pads and 200 nm thick PEDOT:PSS microelectrodes (50 μm in diameter) on laser patterned PET substrates, followed by parylene C encapsulation. The 10 microelectrodes in the MEA spread over a window with a large field of view of ∼45 mm 2 for simultaneous optical imaging.…”

Section: Soft Transparent Meas For Neural and Cardiac Applicationsmentioning

confidence: 99%

Recent Progress on Transparent Microelectrode-Based Soft Bioelectronic Devices for Neuroscience and Cardiac Research

2023

ACS Appl. Bio Mater.

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Transparent microelectrodes have emerged as promising tools to combine electrical and optical sensing and modulation modalities in many areas of biological and biomedical research. Compared to conventional opaque microelectrodes, they offer a number of specific advantages that can enable advances in functionality and performance. In addition to optical transparency, the mechanical softness feature is desired to minimize foreign body responses, increase biocompatibility, and avoid loss of functionality. In this review, we present recent research from the past several years on transparent microelectrode-based soft bioelectronic devices with an emphasis on their material properties and advanced device designs, as well as multimodal application scenarios for neuroscience and cardiology. First, we introduce material candidates with proper electrical, optical, and mechanical properties for soft transparent microelectrodes. We then discuss examples of soft transparent microelectrode arrays tailored to combine electrical recording and/or stimulation with optical imaging and/or optogenetic modulation of the brain and the heart. Next, we summarize the most recent progress on soft opto-electric devices integrating transparent microelectrodes with microscale light-emitting diodes and/or photodetectors into single and hybrid microsystems as powerful tools to explore the brain and heart functions. A brief overview of possible future directions of soft transparent microelectrode-based biointerfaces is provided to conclude the review.

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Section: Soft Transparent Meas For Neural and Cardiac Applicationsmentioning

confidence: 99%

Recent Progress on Transparent Microelectrode-Based Soft Bioelectronic Devices for Neuroscience and Cardiac Research

2023

ACS Appl. Bio Mater.

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“…Instead, the observed neural signal is a population summation of the activity within essentially a 3-dimensional voxel (not just 2 dimensional) that includes the dendrites, somata, and axons within that volume. While typical wide-field Ca 2+ imaging does not report the activity of single cells, there are strategies, such as sparse expression of indicators and/or restriction to the soma, that can provide single cell resolution [ 76 , 77 ].…”

Section: Limitations Of Wide-field Imagingmentioning

confidence: 99%

“…However, chronic studies with transparent ECoG electrodes have been restricted to a single brain region and a small fields of view (~2–5 mm 2 ). To address this limited field of view, transparent, inkjet-printed electrode arrays have been integrated into morphologically conformant transparent polymer skulls (eSee-Shells) [ 77 ]. The electrodes and interconnects are composed of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), a transparent, printable conductor with excellent electrical properties.…”

Section: New Developmentsmentioning

confidence: 99%

Wide-Field Calcium Imaging of Neuronal Network Dynamics In Vivo

Nietz

Popa

Streng

et al. 2022

Biology

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A central tenet of neuroscience is that sensory, motor, and cognitive behaviors are generated by the communications and interactions among neurons, distributed within and across anatomically and functionally distinct brain regions. Therefore, to decipher how the brain plans, learns, and executes behaviors requires characterizing neuronal activity at multiple spatial and temporal scales. This includes simultaneously recording neuronal dynamics at the mesoscale level to understand the interactions among brain regions during different behavioral and brain states. Wide-field Ca2+ imaging, which uses single photon excitation and improved genetically encoded Ca2+ indicators, allows for simultaneous recordings of large brain areas and is proving to be a powerful tool to study neuronal activity at the mesoscopic scale in behaving animals. This review details the techniques used for wide-field Ca2+ imaging and the various approaches employed for the analyses of the rich neuronal-behavioral data sets obtained. Also discussed is how wide-field Ca2+ imaging is providing novel insights into both normal and altered neural processing in disease. Finally, we examine the limitations of the approach and new developments in wide-field Ca2+ imaging that are bringing new capabilities to this important technique for investigating large-scale neuronal dynamics.

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“…Microelectrode arrays made of graphene or carbon nanotubes on transparent parylene C or other substrates have been employed in combination with single- and multiphoton optical imaging in vivo by us and others (Driscoll et al 2021, Kuzum et al 2014, Lu et al 2018, Park et al 2016, Park et al 2014, Thunemann et al 2018, Zhang et al 2018). The second strategy is using non-transparent metal and polymer electrodes on a transparent substrate, where the electrodes are small enough not to produce significant shadows (Donahue et al 2018, Donaldson et al 2022, Ganji et al 2018, Ganji et al 2019, Hossain et al 2020, Qiang et al 2018, Seo et al 2019). Following the second strategy, we have previously developed stable and biocompatible Poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) surface electrode arrays suitable for chronic implantation (Ganji et al 2018, Ganji et al 2019, Hossain et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted September 3, 2022. ; https://doi.org/10.1101/2022.09.01.506113 doi: bioRxiv preprint 6 transparent metal and polymer electrodes on a transparent substrate, where the electrodes are small enough not to produce significant shadows (Donahue et al 2018, Donaldson et al 2022, Ganji et al 2018, Hossain et al 2020, Qiang et al 2018, Seo et al 2019. Following the second strategy, we have previously developed stable and biocompatible Poly(3,4ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) surface electrode arrays suitable for chronic implantation (Ganji et al 2018, Hossain et al 2020.…”

Section: Introductionmentioning

confidence: 99%

Imaging through Windansee electrode arrays reveals a small fraction of local neurons following surface MUA

Thunemann

Hossain

Ness

et al. 2022

Preprint

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Prior studies have shown that neuronal spikes can be recorded with microelectrode arrays placed on the cortical surface. However, the etiology of these spikes remains unclear. Because the top cortical layer (layer 1) contains very few neuronal cell bodies, it has been proposed that these spikes originate from neurons with cell bodies in layer 2. To address this question, we combined two-photon calcium imaging with electrophysiological recordings from the cortical surface in awake mice using chronically implanted PEDOT:PSS electrode arrays on transparent parylene C substrate. Our electrode arrays (termed Windansee) were integrated with cortical windows offering see-through optical access while also providing measurements of local field potentials (LFP) and multiunit activity (MUA) from the cortical surface. To enable longitudinal data acquisition, we have developed a mechanical solution for installation, connectorization, and protection of Windansee devices aiming for an unhindered access for high numerical aperture microscope objectives and a lifetime of several months while worn by a mouse. Contrary to the common notion, our measurements revealed that only a small fraction of layer 2 neurons from the sampled pool (~13%) faithfully followed MUA recorded from the surface above the imaging field-of-view. Surprised by this result, we turned to computational modeling for an alternative explanation of the MUA signal. Using realistic modeling of neurons with back-propagating dendritic properties, we computed the extracellular action potential at the cortical surface due to firing of local cortical neurons and compared the result to that due to axonal inputs to layer 1. Assuming the literature values for the cell/axon density and firing rates, our modeling results show that surface MUA due to axonal inputs is over an order of magnitude larger than that due to firing of layer 2 pyramidal neurons. Thus, a combination of surface MUA recordings with two-photon calcium imaging can provide complementary information about the input to a cortical column and the local circuit response. Cortical layer I plays an important role in integration of a broad range of cortico-cortical, thalamocortical and neuromodulatory inputs. Therefore, detecting their activity as MUA while combining electrode recording with two-photon imaging using optically transparent surface electrode arrays would facilitate studies of the input/output relationship in cortical circuits, inform computational circuit models, and improve the accuracy of the next generation brain-machine interfaces.

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Polymer Skulls With Integrated Transparent Electrode Arrays for Cortex‐Wide Opto‐Electrophysiological Recordings

Cited by 19 publications

References 77 publications

Recent Progress on Transparent Microelectrode-Based Soft Bioelectronic Devices for Neuroscience and Cardiac Research

Recent Progress on Transparent Microelectrode-Based Soft Bioelectronic Devices for Neuroscience and Cardiac Research

Wide-Field Calcium Imaging of Neuronal Network Dynamics In Vivo

Imaging through Windansee electrode arrays reveals a small fraction of local neurons following surface MUA

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