Polymerase Chain Reaction Analysis of Immunoglobulin Gene Rearrangement in Cutaneous Lymphoid Hyperplasias

Bouloc, Anne; Delfau‐Larue, Marie‐Hélène; Lenormand, Bernard; Meunier, Farnaz; Wechsler, Janine; Thomine, E; Revuz, J.; Farcet, Jean‐Pierre; Joly, P.; Bagot, Martine

doi:10.1001/archderm.135.2.168

Cited by 57 publications

(53 citation statements)

References 17 publications

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“…In contrast, false positivity is common in investigation of clonality in suspicious cutaneous involvements as lymphocyte populations are located separately in suspicious cutaneous lesions, and there are limited numbers of lymphoid cells in these lesions [37][38][39]. Thus, the use of inflammatory dermatosis and natural cutaneous cells as negative controls in clonality assessment is recommended to prevent false positivity [38].…”

Section: Discussionmentioning

confidence: 99%

PCR Analysis of IgH and TCR-γ Gene Rearrangements as a Confirmatory Diagnostic Tool for Lymphoproliferative Disorders

Poopak

Valeshabad

Elahi

et al. 2014

Indian J Hematol Blood Transfus

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This study investigates PCR analysis of immunoglobulin heavy chain (IgH) and T cell receptor (TCR) gene rearrangements on paraffin-embedded tissue sections and bone marrow aspirates of patients suspected to have lymphoproliferative disorders but with inconclusive diagnosis in histopathological examination. 130 samples of patients with inconclusive immunohistochemistry results were evaluated for clonal rearrangement of IgH and TCR genes. Based on histopathology examination, the patients were divided into three groups: the first group without any definite diagnosis of lymphoproliferative disorders (60 cases, 46.2 %), the second group suspected to have a lymphoproliferative disorder but in favor of benign disorders (19 cases, 14.6 %) and the third group suspect to lymphoproliferative disorders but relatively in favor of malignant disorders (51 cases, 39.2 %). After DNA extraction and quality control, semi-nested PCR was performed using consensus primers for amplification of TCR-c and CDR-3 regions of IgH genes. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis, and were subject to silver staining. Totally, in over half of the cases (55.4 %), a monoclonal pattern was found in IgH or TCR-c genes rearrangements. Monoclonal IgH gene rearrangement was detected in 48.1 % of patients, whereas monoclonal TCR-c gene rearrangement was found in 33.6 % of them, which was not statistically significant (P = 0.008). Only in 32 patients (24.6 %) were the results of TCR-c and IgH gene rearrangements consistent with respect to the presence (2.3 %) or absence (22.3 %) of monoclonality. Finally, PCR analysis of TCR-c and IgH gene rearrangements led to definite diagnosis in 105 patients (80.8 %), and only 25 cases (19.2 %) remained inconclusive. Our results emphasize the usefulness of gene rearrangement study in cases without a definite diagnosis in immunohistochemistry studies. Multiple PCR analysis results when combined with patient's clinical course and immunohistochemistry can lead to early diagnosis and subsequent therapy.

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Section: Discussionmentioning

confidence: 99%

PCR Analysis of IgH and TCR-γ Gene Rearrangements as a Confirmatory Diagnostic Tool for Lymphoproliferative Disorders

Poopak

Valeshabad

Elahi

et al. 2014

Indian J Hematol Blood Transfus

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“…Although they are defined as benign lesions, even cases showing progression to lymphoma have been reported in the literature (5). Therefore, these lesions should be distinguished from cutaneous lymphomas with histopathological studies (4,6).…”

Section: Discussionmentioning

confidence: 99%

“…Topical or intralesional steroid injection, cryotherapy, interferon alfa, local radiation, and surgical excision can be applied in the treatment of pseudolymphoma (1,3,6). If monoclonality is found in a localized pseudolymphoma lesion, full surgical removal of the lesion and local radiation treatment are also recommended, but there is no evidence to influence the prognosis positively and lesions that are not monoclonal initially can still progress to cutaneous lymphoma (1,6).…”

Section: Discussionmentioning

confidence: 99%

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Nasal Pseudolymphoma

Yıldız¹,

Server²,

Yiğit³

et al. 2016

Istanbul Med J

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Pseudolymphoma (cutaneous lymphoid hyperplasia) caused by various antigenic stimulations in which benign proliferation of polyclonal T and B lymphocytes is observed. Pseudolymphoma lesions have similar clinical and histopathological findings to those of cutaneous lymphoma. Some studies have reported the conversion potential of pseudolymphomas to cutaneous lymphomas. Nasal Pseudolymphoma etiology, clinical features, diagnosis and therapy is discussed in male patients diagnosed with spontaneous Pseudolymphoma at the age of 45.

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“…Hafif atrofik epidermis ile birlikte epidermal-dermal alanda çok dar sağlam bir bölge bırakarak tüm dermisi infiltre eden küçük matür lenfositik hücre infiltrasyonu, arada plazma hücreleri ile bir kaç alanda germinal merkezi belirgin lenfoid folikül yapısının görünümü. Psödolenfomalar klinikopatolojik korelasyonlarından dolayı histokimyasal çalışmalar ile lenfomalardan ayırt edilmelidir (9). Psödolenfomaların aksine kutanöz lenfomalar genellikle generalize ve ilerleyici seyir gösterirler (8).…”

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