Polymerase chain reaction-based detection of myc transduction in feline leukemia virus-infected cats

Sumi, Ryosuke; Miyake, Ariko; Endo, Taiji; Ohsato, Yoshiharu; Ngo, Minh Ha; Nishigaki, Kazuo

doi:10.1007/s00705-018-3721-1

Cited by 3 publications

(3 citation statements)

References 35 publications

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“…Other studies have shown that mediastinal lymphoma develops in young-aged cats [ 7 , 18 , 25 ] but there is no available information on FeLV-antigen-positive cats. The mechanism of FeLV-antigen-positive cats causing lymphoma is through the insertion of a provirus at many different sites in the host’s genome near a cellular oncogene (most commonly myc ) [ 14 , 29 , 30 , 31 ]. This finding should be investigated further in terms of clinical outcomes in feline lymphoma with FeLV-antigen-positive status.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness and Adverse Events of Cyclophosphamide, Vincristine, and Prednisolone Chemotherapy in Feline Mediastinal Lymphoma Naturally Infected with Feline Leukemia Virus

Sunpongsri

Kovitvadhi

Rattanasrisomporn

et al. 2022

Animals

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Feline leukemia virus (FeLV) infection is considered a poor prognostic factor for feline lymphoma. This study investigated the prevalence of cats suffering from feline lymphoma with natural infection of the feline leukemia virus, as well as clinical signs, adverse events, and survival time after cyclophosphamide, vincristine and prednisolone (COP) chemotherapy. This retrospective study involved 92 cats diagnosed with mediastinal or mediastinal plus other anatomical sites of lymphoma and treated with COP chemotherapy. FeLV-antigen-positive was observed in all cats. Clinical signs and adverse events were observed after the 1st, 2nd, and 3rd inductions. Clinical signs improved after the 3rd induction of COP chemotherapy. The response rate was 96.74% (81.52% complete response, 15.22% partial response, and 3.26% no response). The overall median survival time was 338 days (range 62–1057 days). The overall response rate and median survival time of cats with feline lymphoma that were FeLV-antigen-positive and treated with COP chemotherapy were higher than from other studies. This study found that cats aged <4 years survived longer than those aged at least 4 years. Anemia (before COP), azotemia (after 2nd induction), and elevated alanine aminotransferase (after 1st induction) were associated with an increased chance of mortality.

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Section: Discussionmentioning

confidence: 99%

Effectiveness and Adverse Events of Cyclophosphamide, Vincristine, and Prednisolone Chemotherapy in Feline Mediastinal Lymphoma Naturally Infected with Feline Leukemia Virus

Sunpongsri

Kovitvadhi

Rattanasrisomporn

et al. 2022

Animals

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“…The existing literature regarding the quantitative detection of FeLV virus in various reticuloendothelial tissues is profoundly limited. Most of published works about the molecular detection of FeLV have focused on the presence of FeLV in the peripheral blood [1, 2, 4, 18–20]. In this study, we evaluated the presence of FeLV in the whole blood, bone marrow and spleen, and tried to comparatively quantitate the number of viral RNA by RT-qPCR in these lymphohematogenic tissues of cats with hematological cytopenias.…”

Section: Discussionmentioning

confidence: 99%

“…Feline leukemia virus (FeLV) is a retrovirus belonging to the family Retroviridae , subfamily Orthoretrovirinae, genus Gammaretroviru s, which is responsible for infection, tumor development and immunological dysfunction in domestic cats [1–4]. Viremic cats serve as sources of infection for other cats, which can be transmitted via saliva, nasal secretions, urine, feces, and milk [5].…”

Section: Introductionmentioning

confidence: 99%

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias

et al. 2019

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BackgroundFeline leukemia virus (FeLV) is a serious viral infection in cats. FeLV is found in some tissues, such as spleen, lymph nodes and epithelial tissues. However, there is controversy about the organ in which the virus can be reliably detected in infected cats. The purpose of this study was to determine the level of viral infection in hemolymphatic tissues, including blood, bone marrow and spleen by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR).ResultsA total of 31 cats with clinical signs of FeLV infection associated with at least a single lineage hematologic cytopenia were included in this study. Peripheral blood, bone marrow and spleen samples were obtained from each cat. Complete blood counts, biochemical tests, and a rapid test to detect FeLV p27 antigen in blood samples of cats were performed. Of 31 cats, 9 had anemia alone, 4 had thrombocytopenia alone, 2 had neutropenia alone, 9 had bicytopenia of anemia and thrombocytopenia, 3 had bicytopenia of anemia and neutropenia, and 4 had pancytopenia. FeLV RNA was then detected by RT-qPCR in the whole blood, bone marrow and spleen. Viral RNA copy numbers were detected in all cats by RT-qPCR whereas 24 out of 31 cats were positive for the serum FeLV antigen. We detected a significantly greater number of viral RNA in the spleen compared with the whole blood and bone marrow.ConclusionSpleen is a site where FeLV is most frequently detected in cats with hematologic cytopenias.

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What’s New in Feline Leukemia Virus Infection

Hartmann¹,

Hofmann‐Lehmann

2020

Veterinary Clinics of North America: Small Animal Practice

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Polymerase chain reaction-based detection of myc transduction in feline leukemia virus-infected cats

Cited by 3 publications

References 35 publications

Effectiveness and Adverse Events of Cyclophosphamide, Vincristine, and Prednisolone Chemotherapy in Feline Mediastinal Lymphoma Naturally Infected with Feline Leukemia Virus

Effectiveness and Adverse Events of Cyclophosphamide, Vincristine, and Prednisolone Chemotherapy in Feline Mediastinal Lymphoma Naturally Infected with Feline Leukemia Virus

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias

What’s New in Feline Leukemia Virus Infection

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