Polymerase chain reaction detection of Listeria monocytogenes using oligonucleotide primers targeting actA gene

Zhou, Xiaohui; Jiao, Xinan

doi:10.1016/j.foodcont.2004.01.001

Cited by 34 publications

(20 citation statements)

References 27 publications

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“…monocytogenes specific actA gene was reproduced by using specific designed primers (5'-GCTGATTTAAGAGA TAGAGGAACA-3'and 5'-TTTATGTGGTTATTTGCTGTC -3') [17] . The PCR program consisted of an initial denaturation step at 94°C for 2 min, followed by 35 cycles of DNA denaturation at 94°C for 60 s, primer annealing at 50°C for 60s, and primer extension at 72°C for 60 s. PCR products were analysed by gel electrophoresis with 1.5% agarose (Sigma-Aldrich, USA) and visualised.…”

Section: Pcrmentioning

confidence: 99%

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

İkiz¹,

Dümen²,

Kahraman³

et al. 2016

Kafkas Univ Vet Fak Derg

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The present study was conducted to investigate the presence of Salmonella spp. and Listeria monocytogenes in 700 seafood (400 raw fish, 100 raw shrimps and 200 raw molluscs) collected from retailers. Isolations were performed by conventional culture methods. The isolates were also confirmed by PCR assays. Salmonella spp. and L. monocytogenes were detected in 9.9% and 3.86% fish and shellfish samples, respectively. The highest rates of Salmonella spp. (12.5%) were found in fish samples and L. monocytogenes (7.0%) were in shrimp samples. Therefore, it is essential to ensure improving the quality of production technology from fishing to retail outlet and developing the sanitation conditions of food contact surfaces and handling areas. Keywords

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Section: Pcrmentioning

confidence: 99%

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

İkiz¹,

Dümen²,

Kahraman³

et al. 2016

Kafkas Univ Vet Fak Derg

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show abstract

“…The PCR procedures used in this study have been described previously (Zhou & Jiao, 2005). Briefly, 1 mL of pure culture of L. monocytogenes was centrifuged at 13,000g for 5 min at room temperature.…”

Section: Dna Extraction and Pcr Conditionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Listeria species isolated from milk and dairy products in Iran

Ameri²,

2010

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“…The PCR procedures used in this study have been described previously (Zhou and Jiao 2005). Briefly, 1 ml pure culture of L. monocytogenes was centrifuged at 13,000 g for 5 min at room temperature.…”

Section: Dna Extraction and Pcr Conditionsmentioning

confidence: 99%

Prevalence of Listeria species in fresh and frozen fish and shrimp in Iran

2011

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The objective of this study was to determine the prevalence of Listeria spp. in sea food samples in Isfahan and Shahrekord, Iran. From April 2009 to January 2010, a total of 264 samples of fresh and frozen fish and shrimp samples were obtained from randomly selected retail stores in Isfahan and Shahrekord, Iran. The samples were tested for the presence of Listeria spp. using a selective enrichment and isolation protocol recommended by the United States Department of Agriculture. Only Listeria monocytogenes isolates identified by bacteriological methods were tested by polymerase chain reaction (PCR). Using conventional bacteriologic methods, out of the total of 264 samples examined, 20 (7.6%) were found to be positive for Listeria. Listeria species were isolated in 7.5%, 4.2%, 11.7% and 6.6% of fresh fish, frozen fish, fresh shrimp and frozen shrimp samples, respectively. Listeria monocytogenes and L. innocua were detected in 1.9% and 5.7% of the samples analyzed, respectively. All five Listeria strains identified as L. monocytogenes were also positive using PCR. Consumption of these sea foods, either raw or undercooked, may contribute to food-borne illness in Iran. Also, L. monocytotenes in raw seafood may pose a health risk in kitchens if contaminating ready-to-eat food.

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Polymerase chain reaction detection of Listeria monocytogenes using oligonucleotide primers targeting actA gene

Cited by 34 publications

References 27 publications

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

Deniz Ürünlerinde Salmonella spp. ve Listeria monocytogenes Varlığının Kültürel Metotlar ve PCR İle Araştırılması

Prevalence and antimicrobial resistance of Listeria species isolated from milk and dairy products in Iran

Prevalence of Listeria species in fresh and frozen fish and shrimp in Iran

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