Prevalence of Listeria species in fresh and frozen fish and shrimp in Iran

Rahimi, Ebrahim; Shakerian, Amir; Raissy, Mehdi

doi:10.1007/s13213-011-0222-9

Cited by 26 publications

(13 citation statements)

References 31 publications

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“…monocytogenes in dumplings/steamed stuffed bun was 32.1% (27/84), and that in frozen meats was 54.5% (61/112). These values are remarkably higher than those reported in previous studies [ 35 , 36 ]. In contrast, the mean level of L .…”

Section: Resultscontrasting

confidence: 78%

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Zhang

et al. 2015

PLoS ONE

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The prevalence and levels of Listeria monocytogenes in retail raw foods covering most provincial capitals in China were studied with testing of 1036 samples of vegetables, edible mushrooms, raw meat, aquatic products and quick-frozen products from September 2012 to January 2014. The total prevalence of Listeria monocytogenes was 20.0% (207/1036), and the most probable number (MPN) values of 65.7% of the positive samples ranged from 0.3 to 110 MPN/g. Geographical differences were observed in this survey, and the results of both qualitative and quantitative methods indicated that the levels in the samples from North China were higher than those in the samples from South China. A total of 248 isolates were analyzed, of which approximately half belonged to molecular serogroup 1/2a-3a (45.2%), followed by 1/2b-3b-7 (30.6%), 1/2c-3c (16.1%), 4b-4d-4e (5.2%) and 4a-4c (2.8%). Most of the isolates carried hly (100%), inlB (98.8%), inlA (99.6%), inlC (98.0%) and inlJ (99.2%), and 44.8% of the isolates were llsX-positive. Seventeen epidemic clones (ECs) were detected, with 7 strains belonging to ECI (2.8%) and 10 belonging to ECIII (4.03%). Resistance to clindamycin (46.8%) was commonly observed, and 59 strains (23.8%) were susceptible to all 14 tested antibiotics, whereas 84 (33.9%) showed an intermediate level of resistance or were resistant to two or more antibiotics, including 7 multi-resistant strains that exhibited resistance to more than 10 antibiotics. The data obtained in the present study provides useful information for assessment of the possible risk posed to Chinese consumers, and this information will have a significant public health impact in China. Furthermore, the presence of virulence markers, epidemic clones, as well as the antibiotic resistance amongst the isolates strongly implies that many of these strains might be capable of causing listeriosis, and more accurate treatment of human listeriosis with effective antibiotics should be considered. This research represents a more full-scale and systematical investigation of the prevalence of L. monocytogenes in retail raw foods in China, and it provides baseline information for Chinese regulatory authorities that will aid in the formulation of a regulatory framework for controlling L. monocytogenes with the aim of improving the microbiological safety of raw foods.

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Section: Resultscontrasting

confidence: 78%

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Zhang

et al. 2015

PLoS ONE

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“…However, our findings showed higher prevalence of Listeria spp. compared with the previous studies [ 43 , 44 ].…”

Section: Resultsmentioning

confidence: 80%

Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets

et al. 2015

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BackgroundThe aim of this study was to investigate the prevalence and characterization of Listeria species and Listeria monocytogenes isolated from raw fish and open-air fish market environments. Eight hundred and sixty two samples including raw fish and fish market environments (samples from workers’ hands, workers’ knives, containers and work surface) were collected from the open-air fish markets in the Northern region of Iran.ResultsListeria spp. was isolated from 104/488 (21.3 %) raw fish and 29/374 (7.8 %) of samples from open-air fish market environment. The isolates of Listeria spp. included L. innocua (35.3 %), L. monocytogenes (32.3 %), L. seeligeri (18 %), and L. ivanovii (14.3 %). Of the 43 L. monocytogenes isolates, 31 (72.1 %), 10 (23.3 %) and 2 (4.7 %) belonged to serovars 1/2a, 4b, and 1/2b, respectively. The inlA, inlB, inlC, inlJ, actA, hlyA, iap, plcA, and prfA virulence-associated genes were detected in almost all of the L. monocytogenes isolates. The Listeria spp. isolates showed high resistance against tetracycline (23.3 %), penicillin G, and cephalothin (each 16.5 %). Besides, we observed significant resistance level to tetracycline (27.9 %), ampicillin (20.9 %), cephalothin, penicillin G, and streptomycin (each 16.3 %) in the L. monocytogenes isolates. All of the isolates were susceptible to cefotaxime, gentamicin, kanamycin, and pefloxacin. We found that tetM (25.6 %), tetA (23.3 %), ampC (14 %), and penA (11.6 %) were the most prevalent antibiotic resistance genes in the L. monocytogenes isolates.ConclusionsRecovery of potentially pathogenic L. monocytogenes from raw fish and environment of open-air fish market samples in this study is a convincing evidence for the zoonotic potential of listeriosis.

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“…Finally, the prevalence of Listeria species in fresh and frozen fish and shrimp was studies in Iran by Rahimi et al (2012). Listeria species were isolated in 7.5, 4.2, 11.7 and 6.6% of fresh fish, frozen fish, fresh shrimp and frozen shrimp, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Listeria species were isolated in 7.5, 4.2, 11.7 and 6.6% of fresh fish, frozen fish, fresh shrimp and frozen shrimp, respectively. Almost 2% of identified species were L. monocytogenes which led to the conclusion that consumption of sea food either raw or frozen may lead to food borne illnesses in Iran (Rahimi et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Virulence and antimicrobial susceptibility profile of Listeria monocytogenes isolated from frozen vegetables available in the Egyptian market

Mohamed¹,

Abdelmonem²,

Amin³

2018

Afr. J. Microbiol. Res.

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Listeria monocytogenes is among the most important foodborne pathogens. It may enter foodprocessing environments through raw materials, handlers or equipment and may persist due to ineffective cleaning or sanitation. The bacterium can be isolated from both frozen vegetables and fresh food substances. This study aimed to estimate the prevalence of L. monocytogenes in spices and frozen vegetables and screen for some virulence factors and drug-resistance determinants of the isolated bacteria. First, conventional microbiological methods were used for the isolation and identification of bacteria. Next, the identity of isolated bacteria was confirmed by molecular techniques, and the virulence genes iap and hlyA were identified by real-time polymerase chain reaction (PCR). The hemolytic activity of the isolates was assessed by cultivation on sheep blood agar. Furthermore, the antimicrobial susceptibility of confirmed L. monocytogenes isolates was tested by the disk diffusion method against 10 antibiotics. Out of 331 vegetable samples, 47 isolates were confirmed to contain L. monocytogenes, whereas none of 40 spice samples tested positive. All isolates were positive for iap and hlyA genes. Susceptibility testing indicated that all isolates were sensitive to trimethoprim/ sulfamethoxazole, but only 36% were sensitive to penicillin G, while 100% and 70% showed intermediate resistance to chloramphenicol and erythromycin, respectively. All tested isolates were resistant to amoxicillin, gentamicin and norfloxacin; on the other hand, 90, 86 and 84% of the tested strains were resistant to ciprofloxacin, ceftazidime/clavulanic acid and amikacin, respectively. In summary, L. monocytogenes isolates disseminated in frozen vegetable samples from the Egyptian market were highly virulent, entirely multiple-drug resistant and were enriched in iron-containing vegetables. Since L. monocytogenes is primarily pathogenic to humans and causes a life-threatening disease, there is a potential infection risk for people who usually deal with frozen vegetables before cooking. Hence, surveillance to L. monocytogenes in frozen products, together with implementation of tight measures would be valuable in preventing listeriosis, and are highly recommended.

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Prevalence of Listeria species in fresh and frozen fish and shrimp in Iran

Cited by 26 publications

References 31 publications

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China

Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets

Virulence and antimicrobial susceptibility profile of Listeria monocytogenes isolated from frozen vegetables available in the Egyptian market

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