Polymerase chain reaction for non-invasive diagnosis of brain mass lesions caused by Mycobacterium tuberculosis: Report of five cases in human immunodeficiency virus-positive subjects

Monno, Laura; Angarano, Gioacchino; Romanelli, Chiara; Giannelli, Alessio; Appice, A.; Carbonara, Sergio; Costa, Danila; Pastore, Giuseppe

doi:10.1016/s0962-8479(96)90014-3

Cited by 12 publications

(5 citation statements)

References 18 publications

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“…The PCR assay for the hup B gene [20][21][22] used in this study could fulfill two conditionsa rapid means of diagnosis of tuberculous meningitis and also as a tool for differentiation between M. tuberculosis and M. bovis. Various workers using different PCR targets in pediatric CSF samples, for diagnosis of tuberculous meningitis, have shown sensitivity ranging from 40-100% and specificity of 80-100% [36,37]. In all these studies, AFB smear and culture results were very poor.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of a hup B gene based diagnostic PCR with other conventional techniques for pediatric tuberculous meningitis

Nambam

Prasad

Sherwal

et al. 2015

J Pediatr Infect Dis

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Tuberculous meningitis, an extrapulmonary manifestation of tuberculosis, is associated with poor prognosis and high mortality in the pediatric age group with delayed treatment. The main causative agent in humans is Mycobacterium tuberculosis but other members of the M. tuberculosis complex especially Mycobacterium bovis, have also been implicated as potential human pathogens. Our study utilizes the hup B gene (Rv2986c in M. tuberculosis and Mb3010c in M. bovis) as a target for a two-step 'in house' nested polymerase chain reaction (PCR) in cerebrospinal fluid samples and can differentiate between M. tuberculosis and M. bovis. The products obtained for M. tuberculosis and M. bovis are 116 and 89 base pair respectively due to a 27 base pair deletion at the C-terminal of hup B gene of M. bovis. Fifty cases, suggestive of tuberculous meningitis, and fifty controls (admitted for other causes) were chosen as per Modified Ahuja's criteria and correlated with response to therapy. They were regrouped retrospectively as definitive tuberculous meningitis [25] and non-tuberculous meningitis [75]. Of the non-tuberculous meningitis category, 45 patients were diagnosed as pyogenic meningitis while the rest were diagnosed as febrile seizures [16], hypocalcemic seizures [11] and enteric encephalopathy [3]. Mycobacterial DNA extracted from cerebrospinal fluid samples were subjected to a two-step nested PCR. A sensitivity of 92% and specificity of 98.7% was obtained. M. tuberculosis and M. bovis infection was seen in 56% and 35% of the PCR positive cases respectively while 9% showed co-infection with both species. Thus this novel gene target shows promising potential as a rapid molecular diagnostic aid in pediatric tuberculous meningitis where rapid institution of antituberculosis therapy is essential.

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Section: Discussionmentioning

confidence: 99%

Comparative analysis of a hup B gene based diagnostic PCR with other conventional techniques for pediatric tuberculous meningitis

Nambam

Prasad

Sherwal

et al. 2015

J Pediatr Infect Dis

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“…30,31 A small study in five patients with AIDS, using PCR for M. tuberculosis in cerebrospinal fluid, allowed identification of cerebral tuberculosis without meningitis. 32 Another study in 27 patients presenting with bacterial abscesses and tuberculous abscesses, all of them without HIV infection, reported that magnetic resonance spectroscopy and magnetization transfer magnetic resonance imaging could differentiate between etiologies. 33 Further studies must be carried out to determine the real value of those diagnostic strategies in HIV-infected patients and tuberculous brain abscess.…”

Section: Discussionmentioning

confidence: 99%

Tuberculous brain abscess in AIDS patients: report of three cases and literature review

Vidal

Oliveira

Filho

et al. 2005

International Journal of Infectious Diseases

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“…Some investigators [15] reported that PCR for detection of M. tuberculosis complex in CSF was successfully used in the diagnosis of tuberculous brain lesions in 5 patients without meningitis. (Two diagnoses were made on the basis of positive culture results, 1 was biopsy proven, and 2 were based on treatment response).…”

Section: Discussionmentioning

confidence: 99%

New Times for an Old Disease: Intracranial Mass Lesions Caused by Mycobacterium tuberculosis in 5 HIV-Negative African Immigrants

Ripamonti¹,

Barbó²,

Rizzi³

et al. 2004

Clinical Infectious Diseases

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Polymerase chain reaction for non-invasive diagnosis of brain mass lesions caused by Mycobacterium tuberculosis: Report of five cases in human immunodeficiency virus-positive subjects

Cited by 12 publications

References 18 publications

Comparative analysis of a hup B gene based diagnostic PCR with other conventional techniques for pediatric tuberculous meningitis

Comparative analysis of a hup B gene based diagnostic PCR with other conventional techniques for pediatric tuberculous meningitis

Tuberculous brain abscess in AIDS patients: report of three cases and literature review

New Times for an Old Disease: Intracranial Mass Lesions Caused by Mycobacterium tuberculosis in 5 HIV-Negative African Immigrants

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