Polymerase chain reaction versus enzyme-linked immunosorbent assay in detection of &lt;i&gt;Chlamydia trachomatis&lt;/i&gt; infection among gynaecological patients in southwestern Nigeria

Ajani, Tinuade Adesola; Anaedobe, Chinenye Gloria; Oluwasola, Timothy A.O.; Ajani, Mustapha Akanji; Fayemiwo, Samuel A.; Bakare, Rasheed

doi:10.4314/ajcem.v19i2.1

Cited by 2 publications

(1 citation statement)

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“…Indeed, a study has shown that the PCR technique is more sensitive and more specific than the ELISA technique, which was prone to false positive results. 15,16 The objective of this study was to uncover epidemiological data that may serve as a useful tool for the better management of C. trachomatis infections in women in West Cameroon.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and genotyping of Chlamydia trachomatis circulating in women of Western Cameroon: a cross-sectional study

Gomseu¹,

Dadwal²,

Tamokou

et al. 2021

Int J Community Med Public Health

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Background: In Cameroon, C. trachomatis screening is not routinely practiced, and its epidemiology is still unexplored. The present study aimed to determine the prevalence of C. trachomatis infection, its risk factors and the genotypes circulating in the West Cameroon region.Methods: A cross-sectional study was carried out amongst patients in five district hospitals in the West region of Cameroon. Endocervical samples were collected from women visiting the hospitals forantenatal, prenuptial and contraception consultations and at least 18 years old, sexually active, and non-menstruating. The molecular detection of C. trachomatis was performed using conventional polymerase chain reaction (PCR) followed by sequencing of the ompA gene.Results: The prevalence of C. trachomatis infection was determined to be 11.47%. Having sex for the first time between the ages of 15 and 17 (OR=1.683, 95% CI: 1.1-2.5), non-usage of condom (OR=1.622, 95% CI: 1.2-2.1), being single (OR=1.263, 95% CI: 1.0-1.5) and age range 18-30 years (OR=1.426, 95% CI: 1.1-1.8) were risk factors for C. trachomatis infection. Three genotypes of C. trachomatis circulated in West Cameroon viz. D (49%), E (29.4%) and G (21.6%).Conclusions: This study revealed that, three genotypes; D (dominant), E and G were identified circulating in the population of the study area. This information may be important for controlling the dissemination of C. trachomatis infection in West Cameroon as well as strategizing the therapeutic approach.

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Section: Introductionmentioning

confidence: 99%