Polymères photoréticulables à unités cinnamiques, 3. Photoréticulation

Remmas, Miloud; Soutif, Jean‐Claude; Brosse, Jean‐Claude

doi:10.1002/macp.1991.021920820

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…One of the most prominent reactions applied for the crosslinking of polymers is the UV‐induced [2 + 2] cycloaddition reaction of cinnamic acid derivatives. Because the pioneering work of Schmidt and coworkers17–19 as well as many others,20–26 the topochemical dimerization reaction of cinnamic acid and its derivatives has become a useful tool in polymer chemistry27–31 and material science 8, 11, 32–36. Herein, we report the synthesis and characterization of statistical and amphiphilic block‐random copolymers prepared by ring opening metathesis polymerization (ROMP).…”

Section: Introductionmentioning

confidence: 99%

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

et al. 2011

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We present a method for the specific labelling of fusion proteins with synthetic fluorophores in Zebrafish. The method uses the SNAP-tag technology and O 6 -benzylguanine derivatives of various synthetic fluorophores. We demonstrate how the method can be used to label subcellular structures in Zebrafish such as the nucleus, cell membranes, and endosomal membranes. The stability of the synthetic fluorophores makes them attractive choices for long-term imaging and allows, unlike most of the autofluorescent proteins, the use of acid fixatives such as trichloroacetic acid. Furthermore, the use of O 6 -benzylguanine derivatives bearing caged fluorescein allows cell lineage tracing through photo-deprotection of the fluorophore and its detection either through fluorescence microscopy or through immunohistochemistry after fixation using anti-fluorescein antibodies. Developmental Dynamics 240:820-827,

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Section: Introductionmentioning

confidence: 99%

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

et al. 2011

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“…8,[13][14][15][16] One of the most prominent reactions applied for the crosslinking of polymers is the UV-induced [2 þ 2] cycloaddition reaction of cinnamic acid derivatives. Because the pioneering work of Schmidt and coworkers [17][18][19] as well as many others, [20][21][22][23][24][25][26] the topochemical dimerization reaction of cinnamic acid and its derivatives has become a useful tool in polymer chemistry [27][28][29][30][31] and material science. 8,11,[32][33][34][35][36] Herein, we report the synthesis and characterization of statistical and amphiphilic block-random copolymers prepared by ring opening metathesis polymerization (ROMP).…”

Section: Introductionmentioning

confidence: 99%

UV‐induced crosslinking of ring opening metathesis block copolymer micelles

Sandholzer

Bichler

Stelzer

et al. 2008

J. Polym. Sci. A Polym. Chem.

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Statistical and amphiphilic block copolymers bearing cinnamoyl groups were prepared by ring opening metathesis polymerization (ROMP). The UV‐induced [2 + 2] cycloaddition reaction of polymer bound cinnamic acid groups was studied in polymer thin films as well as in block copolymer micelles. In both cases, exposure to UV‐light for 10 min led to a crosslinking conversion of about 60%, as determined by FT‐IR spectroscopy and UV–vis absorption measurements. Time based IR‐spectroscopy revealed a maximum conversion of 78% reached after an irradiation time of about 16 min. For micelles obtained from polymers bearing 5 mol % or more cinnamoyl groups, the crosslinking reaction proceeded smoothly, yielding in crosslinked particles which were stable in a non‐selective solvent (CHCl3). Diameters determined by dynamic light scattering in the selective solvent (MeOH) were similar for both, non‐crosslinked and crosslinked micelles, whereas diameters of crosslinked micelles in the non‐selective solvent (CHCl3) were significantly larger compared to MeOH samples. This strategy of direct self assembly of block‐copolymers in a selective solvent followed by “clean” crosslinking, without the need for additional crosslinking reagents or crosslinking initiators, provides a straight forward approach toward ROMP‐based polymeric nano‐particles. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 2402–2413, 2008

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