Polymeric alginate nanoparticles containing the local anesthetic bupivacaine

Grillo, Renato; Melo, Nathalie Ferreira Silva de; Araújo, Daniele Ribeiro de; Paula, Eneida de; Rosa, André Henrique; Fraceto, Leonardo Fernandes

doi:10.3109/10611861003649738

Cited by 88 publications

(76 citation statements)

References 57 publications

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“…1D). The infrared spectrum shows the PLGA polymer, with characteristic bands of ester carbonyl (C = O) stretch at 1735 cm 21 and O-H stretch at 3440 cm…”

Section: Resultsmentioning

confidence: 99%

“…The release of 15d-PGJ 2 from loaded NC used a system composed of two compartments (donor and acceptor), separated by a cellulose membrane with a molecular exclusion pore size of 1 kDa. The system was constantly stirred, under sink conditions (20)(21)(22). Aliquots were withdrawn from the acceptor compartment at intervals of 15, 30, and 60 min, over a total period of 360 min, and analyzed by HPLC.…”

Section: In Vitro Release Kinetics Assaysmentioning

confidence: 99%

“…The instrument was operated in transmittance mode, with 32 accumulations covering the frequency range 4000 to 400 cm 21 .…”

Section: Attenuated Total Reflectance-fourier Transform Infrared Analmentioning

confidence: 99%

“…1C). It also shows the carboxyl (C = O) stretch band at 1710 cm 21 and saturated carbon (C-H) stretch bands between 2800 and 3000 cm 21 . Characteristic peaks of NC (Fig.…”

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Exogenous Administration of 15d-PGJ2–Loaded Nanocapsules Inhibits Bone Resorption in a Mouse Periodontitis Model

Napimoga

Silva

Carregaro

et al. 2012

The Journal of Immunology

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The 15-deoxy-Δ12,14-PG J2 (15d-PGJ2) has demonstrated excellent anti-inflammatory results in different experimental models. It can be used with a polymeric nanostructure system for modified drug release, which can change the therapeutic properties of the active principle, leading to increased stability and slower/prolonged release. The aim of the current study was to test a nanotechnological formulation as a carrier for 15d-PGJ2, and to investigate the immunomodulatory effects of this formulation in a mouse periodontitis model. Poly (D,L-lactide-coglycolide) nanocapsules (NC) were used to encapsulate 15d-PGJ2. BALB/c mice were infected on days 0, 2, and 4 with Aggregatibacter actinomycetemcomitans and divided into groups (n = 5) that were treated daily during 15 d with 1, 3, or 10 μg/kg 15d-PGJ2-NC. The animals were sacrificed, the submandibular lymph nodes were removed for FACS analysis, and the jaws were analyzed for bone resorption by morphometry. Immunoinflammatory markers in the gingival tissue were analyzed by reverse transcriptase-quantitative PCR, Western blotting, or ELISA. Infected animals treated with the 15d-PGJ2-NC presented lower bone resorption than infected animals without treatment (p < 0.05). Furthermore, infected animals treated with 10 μg/kg 15d-PGJ2-NC had a reduction of CD4+CD25+FOXP3+ cells and CD4/CD8 ratio in the submandibular lymph node (p < 0.05). Moreover, CD55 was upregulated, whereas RANKL was downregulated in the gingival tissue of the 10 μg/kg treated group (p < 0.05). Several proinflammatory cytokines were decreased in the group treated with 10 μg/kg 15d-PGJ2-NC, and high amounts of 15d-PGJ2 were observed in the gingiva. In conclusion, the 15d-PGJ2-NC formulation presented immunomodulatory effects, decreasing bone resorption and inflammatory responses in a periodontitis mouse model.

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“…1D). The infrared spectrum shows the PLGA polymer, with characteristic bands of ester carbonyl (C = O) stretch at 1735 cm 21 and O-H stretch at 3440 cm…”

Section: Resultsmentioning

confidence: 99%

Section: In Vitro Release Kinetics Assaysmentioning

confidence: 99%

“…The instrument was operated in transmittance mode, with 32 accumulations covering the frequency range 4000 to 400 cm 21 .…”

Section: Attenuated Total Reflectance-fourier Transform Infrared Analmentioning

confidence: 99%

“…1C). It also shows the carboxyl (C = O) stretch band at 1710 cm 21 and saturated carbon (C-H) stretch bands between 2800 and 3000 cm 21 . Characteristic peaks of NC (Fig.…”

mentioning

confidence: 99%

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Exogenous Administration of 15d-PGJ2–Loaded Nanocapsules Inhibits Bone Resorption in a Mouse Periodontitis Model

Napimoga

Silva

Carregaro

et al. 2012

The Journal of Immunology

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“…8 A literatura descreve apenas alguns trabalhos relacionados à liberação de compostos com aplicações em agronegócio, no entanto a abordagem de utilização de nanopartículas poliméricas ainda tem sido muito pouco explorada. [9][10][11] Sistemas de liberação nanoestruturados poliméricos agem como compartimentos transportadores de substâncias ativas e apresentam vantagens que justificam sua aplicação, dentre elas, a boa estabilidade física, química e biológica, fácil preparo e boa reprodutividade, além de serem aplicáveis a uma ampla variedade de substâncias visando melhorar suas propriedades químicas.…”

Section: Introductionunclassified

Nanopartículas de alginato como sistema de liberação para o herbicida clomazone

Silva¹,

Cocenza²,

Melo³

et al. 2010

Quím. Nova

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Recebido em 13/12/09; aceito em 30/6/10; publicado na web em 21/9/10 ALGINATE NANOPARTICLES AS A CONTROLLED RELEASE SYSTEM FOR CLOMAZONE HERBICIDE. In this paper, we describe the preparation of alginate nanoparticles as a delivery system for the herbicide clomazone. Two different methods were investigated and characterized by size distribution, zeta potencial, pH and in vitro release. The alginate/AOT nanoparticles had higher rates of association of the herbicide clomazone than alginate/chitosan nanoparticles. Clomazone release profile, showed a significant difference in release behavior of pure herbicide in solution when compared with herbicide loaded in both alginate nanoparticles. This study is important to construct a biodegradable release system using herbicide for later release into more specific targets, avoiding contamination of environmental matrices.Keywords: environmental chemistry; herbicide; alginate nanoparticles. INTRODUÇÃOHerbicida (de acordo com a etimologia: herbi, erva, e cida matar) é um produto químico utilizado na agricultura para o controle de ervas classificadas como daninhas. Os herbicidas constituem um tipo de pesticida, sendo que as vantagens da utilização deste produto são rapidez de ação, custo reduzido, efeito residual e não revolvimento do solo. Representam hoje os produtos mais amplamente encontrados em recursos hídricos superficiais e subterrâneos do mundo todo, justificado pelo amplo uso em áreas agrícolas e urbanas. Trata-se de uma variedade de moléculas com diferentes propriedades que lhes conferem diferentes graus de persistência ambiental, mobilidade e potenciais tóxico, carcinogênico, mutagênico e teratogênico ou algum efeito endócrino a diversos organismos não alvos, inclusive o ser humano., pertencente ao grupo químico isoxazolidinonas, é indicado para controle em pré-emergência de mono e dicotiledôneas, principalmente feijão, algodão, milho, tabaco, arroz e cana-de-açúcar. É altamente solúvel em água e classificado pela Legislação Brasileira com toxicidade de classe III (média). O herbicida possui grande potencial de poluição aquática em ecossistemas. Trata-se de um agente químico muito usado na região sul do Brasil, principalmente em culturas irrigadas de arroz. 2A fim de desenvolver mecanismos de liberação modificada para herbicidas, testou-se como alternativa a utilização de nanopartículas poliméricas. As nanopartículas poliméricas são sistemas carreadores de substâncias que apresentam diâmetro inferior a 1000 nm e entre as estruturas possíveis destacam-se as nanocápsulas (NC) e as nanoesferas (NE). Esses dois sistemas diferem entre si segundo a composição e organização estrutural. As NC são constituídas por um invólucro polimérico e um núcleo oleoso, podendo o herbicida estar dissolvido neste núcleo e/ou adsorvido à parede polimérica. Já as NE são formadas por uma matriz polimérica e não apresentam óleo em sua composição.3,4 Neste sistema, o herbicida pode ficar retido ou adsorvido.Os herbicidas atualmente empregados também apresentam diversos problemas relacionados ...

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