Polymeric Columns for Liquid Chromatography

Abstract

Polymeric columns are becoming used more frequently in modern liquid chromatography applications as improvements in polymeric packing materials are realized and as more applications are developed for these materials. Modern polymers have overcome earlier problems associated with their use; higher rigidity now allows them to be used at normal eluent flow rates resulting in faster analyses, and improved synthesis techniques have resulted in efficiencies comparable to the best silica materials, sometimes exceeding 100,000 plates/m. In addition, polymers offer distinct advantages over silica packing for particular applications. Because silica packings are readily degraded by aqueous eluents, they are not always suitable for separations involving ionic species. Polymeric columns are particularly useful in determinations of amino acids, peptides, proteins, organic acids, carbohydrates, and inorganic cations and anions. Polymers are also characterized by exceptional lot-to-lot reproducibility. Reproducibility is often problematic with silica packings, particularly the bonded phases.

Investigation of the properties of hypercrosslinked polystyrene as a stationary phase for high-performance liquid chromatography

“…The lesser performance of PS-DVB adsorbents is believed to have several causes [3,9,[35][36][37][38]: -swelling and shrinkage of the adsorbent particles with changing mobile-phase composition; -strong re-re interactions between the PS-DVB adsorbent and sorbates with aromatic rings in the mole- nant zones' and slow adsorption-desorption kinetics; and -slow diffusion of sorbate within the polymer matrix. It has been shown above that the volume of MN-200 particles does not change noticeably in different mobile phases.…”

The Column in Liquid Chromatography

“…Macroporous polymer packings have found increasing use for those applications that silica based packings are inadequate due to their hydrolytic instability or surface heterogeneity [9,12,[103][104][105][106]. The polymeric packings are totally organic and have a more homogeneous surface devoid of strong bonding sites, and depending on their structure, can frequently be used at high and low pH where silica packings are degraded due to the formation of soluble silicates or hydrolysis of siloxane bonded ligands.…”

Trends in Analytical Scale Separations

“…However, Guiochon et al (18) have proposed a bolder scheme more nearly analogous to two-dimensional thin-layer chromatography. Their approach is to use a flat rectangular "column" packed with high-performance packing material through which 19 mobile phase is pumped in c Later, a second mobile pha in a direction perpendiculai Detection is carried out ondiode-array or vidicon imal nary results with this systen ing, but further refinement Preparation of new pack for HPLC is an active ani area of research. This is par of HPLC for separation of where problems of protein are to be avoided.…”

“…This is par of HPLC for separation of where problems of protein are to be avoided. As des review by Regnier (1), mu learned about the HPLC biopolymers, and some gei sions about how biomolecu handled in HPLC are emer, An interesting material I phase chromatography is a rous form of polystyrene/di, available as 10-,um beads withstanding pressures to pounds per square inch (19) tage of this material over ti ylated silica materials is its used with a mobile phase p 13, unlike silica, which has E of about 8. Polystyrene al troublesome surface silb which often lead to unde tailing.…”