Polymeric gene delivery of ischemia-inducible VEGF significantly attenuates infarct size and apoptosis following myocardial infarct

Cardiac Vasculature: Development and Pathology

“…For example, some constructs allow VEGFA levels to be increased specifically during cardiac ischemia. (Lee et al, 2003;Su et al, 2002) and the treated animals have reduced infarct size and increased angiogenesis (Dong et al, 2009;Yockman et al, 2009). Hepatocyte growth factor gene therapy: Human Hepatocyte Growth Factor (hHGF) gene therapy induced angiogenesis in rats and dogs after experimental myocardial infarction (MI) and also improved cardiac function (Ahmet et al, 2002;Ahmet et al, 2003;Cho et al, 2008;Jayasankar et al, 2003;Jin et al, 2004;Li et al, 2003;Taniyama et al, 2002;Yang et al, 2007;Yang et al, 2010).…”

“…Isoforms of VEGFA bind to specific receptors on endothelial cells and play an essential role in angiogenesis (Hao et al, 2007). VEGFA-165 (VEGFA isoform) gene therapy using plasmids in rats (Dong et al, 2009;Yockman et al, 2009) or through non-viral delivery systems in rabbits (Aoki et al, 2000) induces significant neovascularization and improves fractional shortening after modeling myocardial infarction (MI). Concerns with angiogenic therapies exist because VEGFA is a powerful factor whose expression level must be tightly regulated in isoform expression, timing and location.…”

Stem cells and growth factor delivery systems for cardiovascular disease

“…At present, GF delivery in regenerative medicine basically relies upon two strategies: (1) (2) direct delivery by incorporation into a vehicle (Yockman et al, 2009;Layman et al, 2009). Table 1 provides an overview of large animal and main human preclinical and clinical studies of GF delivery for ischemic vascular disease.…”

Approaches in Gene Therapy of Cancer and Cardiovascular Diseases

“…In a mouse model of myocardial infarction, the ultrasoundtargeted gene delivery of VEGF or stem cell factor resulted in increased vascular density and improved myocardial perfusion and ventricular function (Fujii et al, 2009). Yockman et al, used an ischemia-inducible plasmid construct expressing VEGF to treat myocardial ischemia and infarction (Yockman et al, 2009). Ischemia-inducible system was superior to constitutively expressed gene construct in reducing the infarct size.…”