Polymeric nanoparticles surface-complexed with boric acid actively target solid tumors overexpressing sialic acid

Glucosylated Hybrid TiO2/Polymer Nanomaterials for Actively Targeted Sonodynamic Therapy of Cancer

“…[ 65 ] To obtain a more preclinically relevant model to test NP uptake, toxicity, and sonodynamic efficacy and optimize the NP dose and US regimen toward preclinical efficacy studies, here we use a 3D Rh30 cell model, namely a rhabdomyosarcoma spheroid, displaying cellular and cell‐extracellular matrix interactions similar to those in a solid tumor in vivo. [ 66 ] To produce Rh30 cell spheroids, we utilize a modified technique comprising centrifugation and Matrigel, a mixture of extracellular proteins secreted by mouse tumor cells. [ 67 ] The growth of the spheroids is monitored at regular intervals by optical microscopy until the formation of rounded and dense cellular constructs with a size of ≈600 µm ( Figure a).…”