Polymeric Scaffold Based Gene Delivery Strategies to Improve Angiogenesis in Tissue Engineering: A Review

Hadjizadeh, Afra; Ghasemkhah, Farzaneh; Ghasemzaie, Niloofar

doi:10.1080/15583724.2017.1292402

Cited by 32 publications

(18 citation statements)

References 174 publications

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“…The further aim of this study is achieving different release rates through blending polymers and cross‐linking with this aim that the specific release rates are needed for some biomolecules. For instance, each of the stages of angiogenesis is regulated by a balance between stimulatory and inhibitory angiogenic growth factors (GFs) which need different release rates due to having different functionality …”

Section: Introductionmentioning

confidence: 99%

“…For instance, each of the stages of angiogenesis is regulated by a balance between stimulatory and inhibitory angiogenic growth factors (GFs) which need different release rates due to having different functionality. 45 In this study, we assumed that the release rates, mechanical properties, and scaffold-cell interactions will differ at different GT contents as well as by cross-linking. Herein, the core-shell structured scaffolds were designed by combining GT and PCL as the shell layer and/or their cross-linking to evaluate the potential of GT-based core-shell nanofibers for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

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Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Ghasemkhah

Latifi

Hadjizadeh

et al. 2019

J Biomedical Materials Res

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The biomaterials design as core–shell structures opens a new door to the release of susceptible biomolecules in a controllable manner and enables to place natural biomaterials as shell layers to impart the effective biofunctional features at surfaces. In this study, core–shell designed scaffolds were prepared using coaxial electrospinning with hybrid of gelatin (GT)/polycaprolactone (PCL) at different weight ratios as their shell and protein solution as their core, followed by cross‐linking to impart controllable release rates, tunable mechanical properties, and enhanced cytocompatibility. SEM, FM, and TEM confirmed the successful production of uniform core–shell nanofibers and homogeneous protein distribution. Results showed that an increase in GT proportion in the shell resulted in a decrease in fiber diameter, an increase of Young's modulus, and an intense burst release of BSA 0.2% which could be controlled through cross‐linking. The mechanical tests revealed that the GT/PCL combining and cross‐linking improved mechanical properties which correlated with an increase in spreading and proliferation of HUVECs. A slight burst release was also detected from BSA 0.05% and EGF encapsulated GT73P‐cross‐linked scaffold which demonstrated their applicability for a controlled release of dilute proteins. We were able to successfully incorporate two types of protein with different concentrations without supporting polymer into the GT shell to provide scaffolds possessing tunable mechanical properties and controllable release rates through blending with PCL at different ratios and/or cross‐linking. These findings are promising to promote delivery systems of angiogenic growth factors that are needed a sustained release with different rates at each angiogenesis stage. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Ghasemkhah

Latifi

Hadjizadeh

et al. 2019

J Biomedical Materials Res

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“…Then, bovine serum albumin-basic fibroblast growth factor (BSA-bFGF) was loaded in chitosan nanoparticles and entrapment efficiency (EE%) and in vitro release profile were investigated. bFGF is an angiogenic growth factor that has a vital role in angiogenesis and vasculogenesis processes [33,34] and BSA is a physiological protein which can enhance the stability and duration of release profile when combined with other factors [9]. Finally, GelMA/chitosan nanoparticles hydrogel was characterized and its bFGF Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of GelMA/chitosan nanoparticles composite hydrogel for angiogenic growth factor delivery

Modaresifar

Hadjizadeh

Niknejad

2017

Artificial Cells, Nanomedicine, and Biotechnology

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The cellular microenvironment plays a crucial role in improving cell response and function of an engineered tissue. Scaffolds mimicking the native ECM and capable of releasing growth factors are great candidates for tissue engineering applications. Gelatin methacryloyl (GelMA) hydrogel, a photocrosslinkable biomaterial possessing tunable properties, has been widely used in tissue engineering. It has been suggested that incorporating micro/nano carriers in GelMA could provide a sustained release of growth factors. Specifically, chitosan nanoparticles can be used for growth factor delivery due to its biocompatibility, easy method of synthesis, and preventing the biomolecule from degradation. In this study, GelMA/chitosan nanoparticles composite hydrogel was developed to deliver an angiogenic growth factor (bFGF). The hydrogel was prepared by photopolymerization and its chemical and physical properties were characterized. Its degradation and swelling characteristics were also evaluated. The size of nanoparticles was evaluated and the profile of bFGF release from the hydrogel and its effect on the viability of fibroblast cells was studied. The results showed that GelMA/chitosan nanoparticles can significantly promote cell proliferation due to its biocompatible structure and providing a sustained profile of bFGF release. This hydrogel scaffold can be used for efficient delivery of bFGF in various applications and especially for angiogenesis.

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“…These systems in addition to carrying and delivering the desired construct can be effective in bone regeneration by itself. 61 For example, chitosan-based hydrogel have been used in bone regeneration as gene delivery systems to transfer the BMP2 gene. 62…”

Section: Delivery Approachs For Gene Enhanced Regenerationmentioning

confidence: 99%

Gene-Enhanced Personalized Regenerative Medicine for Bone

Alavian

Alizadeh

Ghasemi

2019

J Apple Biotechnol Rep

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Regenerative medicine (RM) is a developing multidisciplinary science that uses different principles and methods of other fields or sciences for tissue regeneration, repair or replacement. Gene therapy refers to transferring genes or gene expression regulator factors for the desired purposes. In some cases, gene therapy plays an important role in regenerative medicine by modulating stem cells from different sources. Genetic heterogeneity of individuals can affect the results of gene therapy or other therapies in RM. This is why personal genomics should be considered in RM and is called personalized regenerative medicine (PRM). The purpose of PRM is to employ strategies and methods tailored to the individual's genetics in order to efficiently reconstruct or substitute various parts of the body. In this study, the strategies and recent advances in bone regeneration such as gene therapy, epigenetic-based therapies, RNA-based therapy and CRISPR/Cas9 system with an attitude to personalized medicine are introduced.

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Polymeric Scaffold Based Gene Delivery Strategies to Improve Angiogenesis in Tissue Engineering: A Review

Cited by 32 publications

References 174 publications

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Design and fabrication of GelMA/chitosan nanoparticles composite hydrogel for angiogenic growth factor delivery

Gene-Enhanced Personalized Regenerative Medicine for Bone

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