Polymeric Thin Films That Resist the Adsorption of Proteins and the Adhesion of Bacteria

Chapman, Robert; Ostuni, Emanuele; Liang, Michael N.; Meluleni, Gloria; Kim, Enoch; Yan, Lin; Pier, Gerald B.; Warren, A.; Whitesides, George M.

doi:10.1021/la001222d

Cited by 344 publications

(297 citation statements)

References 45 publications

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“…In turn, it might be expected that the enthalpic cost of species adsorbing to the polymer brushes and displacing the bound water would not be offset by entropic gain as water escaped to bulk solvent. 28,29 As a consequence, the brushes were less likely to adsorb biopolymers and cells as the polymers chains expanded and acquired an associated bound water layer. As shown in Figure 3, a significant increase of Lewis basicity for Brush 2 took place between 25 and 32 C, while for Brush 3 the value of γ S − changed markedly between 32 and 37 C.…”

Section: Resultsmentioning

confidence: 99%

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

et al. 2017

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We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment. Thermoresponsive poly(N-acryloyl glycinamide)-statpoly(N-phenylacrylamide) (PNAGAm-PNPhAm) brushes with three different monomer ratios were synthesized to give tunable phase transition temperatures (Tp) in solution. Surface energies of surface-grafted brushes of these polymers at 25, 32, 37 and 50 C were calculated from contact angle measurements and atomic force microscopy (AFM) studies confirmed that these polymers were highly extended at temperatures close to Tp in physiologically-relevant media. Importantly, NIH-3T3 cells were attached on the collapsed PNAGAm-PNPhAm brush surface at 30 C after 20 h incubation, while release of cells from the extended brushes was observed within 2 h after the culture temperature was switched to 37 C. Furthermore, the changes in cell attachment followed changes in the Lewis base component of surface energy. The results indicate that, in contrast to the established paradigm of enhanced cell attachment to surfaces where polymers are above a Lower Critical Solution Temperature (LCST), these novel substrates enable detachment of cells from surfaces at temperatures above a UCST. In turn these responsive materials open new avenues for the use of polymer-modified surfaces to control cell attachment for applications in cell manufacture and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

et al. 2017

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“…In order to avoid implant-associated infections, several strategies have been reported with the aim of creating antimicrobial surfaces, such as the development of (i) non-fouling surfaces (surfaces that avoid protein adsorption and cell adhesion) [1,[18][19][20], (ii) surfaces previously colonized with non-pathogenic bacteria [9], (iii) surfaces combined with biocidal substances [21][22][23] and (iv) surfaces combined with antibiotics [24][25][26].…”

Section: Antimicrobial Coatingsmentioning

confidence: 99%

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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a b s t r a c tBacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs' potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and longterm stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.

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“…Functionalization with polymer multilayers allows the introduction of reactive groups, for example, carboxyl acid or amine groups onto the nanotube surface, and these groups may be utilized to attach biospecific ligands [53] and other groups as well. Thus, nonspecific adsorption of biomolecules [54] have been used for applications in biosensors.…”

Section: Preparation Of Aunp-filled Cntsmentioning

confidence: 99%

Carbon Nanotube and Gold‐Based Materials: A Symbiosis

Singh

Premkumar

Shin

et al. 2010

Chemistry A European J

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Carbon nanotubes constitute a novel class of nanomaterials with potential applications in many areas. The attachment of metal nanoparticles to carbon nanotubes is new way to obtain novel hybrid materials with interesting properties for various applications such as catalysts and gas sensors as well as electronic and magnetic devices. Their unique properties such as excellent electronic properties, a good chemical stability, and a large surface area make carbon nanotubes very useful as a support for gold nanoparticles in many potential applications, ranging from advanced catalytic systems through very sensitive electrochemical sensors and biosensors to highly efficient fuel cells. Here we give an overview on the recent progress in this area by exploring the various synthesis approaches and types of assemblies, in which nanotubes can be decorated with gold nanoparticles and explore the diverse applications of the resulting composites.

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Polymeric Thin Films That Resist the Adsorption of Proteins and the Adhesion of Bacteria

Cited by 344 publications

References 45 publications

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Carbon Nanotube and Gold‐Based Materials: A Symbiosis

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