Polymerizable dye for colored particles synthesis with potential use in immunoassays

Preparation of dyed polymer microspheres by a physical–chemical dual-binding method and their application in lateral flow immunoassay

“…The traditional preparation methods of dyed microspheres can be divided into two categories according to the combination of microspheres and dyes: (1) physical methods, including the swelling method [21][22][23] and embedding method; [24][25][26] (2) chemical methods, including the copolymerization method [27][28][29] and surface covalent bonding method. [30][31][32] The swelling method typically penetrates the dye into the polymer chain with the organic solvent, and then the dyes are immobilized aer selectively removing the swelling agent.…”