Polymicrobial tenosynovitis with<i>Pasteurella multocida</i>and other Gram negative bacilli after a Siberian tiger bite

Isotalo, Phillip A.; Edgar, Daniel; Toye, Baldwin

doi:10.1136/jcp.53.11.871

Cited by 43 publications

(43 citation statements)

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“…multocida and P. multocida-like bacteria have occasionally been isolated from the wounds of humans bitten by large cats, like lions and tigers (6,25,29,48,50). Similar organisms have also been isolated from the dental-gingival junction of several species of large cats (50).…”

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Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds

et al. 2005

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To validate the identification of Pasteurella multocida-like bacteria negative for acid formation from sucrose, including isolates from bite wounds caused by large cats, 17 strains were phenotypically and genotypically characterized. Phylogenetic analysis of partially sequenced rpoB and infB genes showed the monophyly of the strains characterized and the reference strains of P. multocida. The sucrose-negative strains formed two groups, one related to reference strains of P. multocida and the other related to a separate species-like group (taxon 45 of Bisgaard). DNA-DNA hybridization further documented the species-like nature of this group. Ribotyping showed the heterogeneity of all strains except four strains that shared the same ribotype and that were isolated from bovine lungs. Phylogenetic analysis by 16S rRNA sequence comparison showed the monophyly of the strains characterized and the reference strains of P. multocida. Two strains isolated from leopard bite wounds were related to the type strain of P. dagmatis; however, they represented a new taxon (taxon 46 of Bisgaard), in accordance with their distinct phenotypic and genotypic identifications. The present study documents that sucrose-negative strains of P. multocida-like bacteria belong to two genotypically distinct groups. The study further confirms the phenotypic heterogeneity of P. multocida strains and documents two new species-like taxa of Pasteurella related to P. multocida. Until diagnostic tools have been further elaborated, special care should be taken in the identification of Pasteurella-like bacteria isolated from bite wounds caused by large cats. The evidence of phenotypic and genotypic divergence calls for the further development of PCR tests and DNA sequencing to document doubtful isolates.Pasteurella multocida has been isolated from a multitude of hosts (26), and different lineages of P. multocida may be responsible for various diseases in both birds and mammals (9, 15). Human infections with P. multocida are in most cases of animal origin and are most often related to the bites of carnivores. However, other types of infections are also occasionally reported (25,28,34).Significant variations in the phenotypic properties of P. multocida have been reported (27), leading to confusion in the definition and identification of this organism. Mutters et al. (37) reclassified the genus Pasteurella on the basis of DNA-DNA hybridization studies. Three clusters of P. multocida showing 84 to 100, 91 to 100, and 89 to 100% DNA reassociation between strains subsequently described as P. multocida subsp. multocida, P. multocida subsp. gallicida, and P. multocida subsp. septica, respectively, were identified. Representatives of the existing capsular types were found to be closely related on the basis of DNA-DNA hybridization (44), despite the diversity of disease manifestations and hosts. This is contradictory to the diversity shown by outer membrane protein profiling (17,18,19), multilocus enzyme electrophoresis (5), and ribotyping (43). The study of Pet...

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Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds

et al. 2005

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“…The tenosynovitis case was polymicrobial in nature and also had a Comamonas species, P. mul- VOL. 42, 2004 GENETIC COMPARISON OF ZOONOTIC BERGEYELLA ZOOHELCUM 291 tocida, and other gram-negative bacteria (12). These clinical cases suggest that B. zoohelcum is an opportunistic pathogen.…”

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confidence: 90%

“…It has also caused cases of septicemia in (i) an 80-year-old diabetic woman, (ii) in a 77-year-old woman suffering from cat associated-severe skin infections, and (iii) in a 33-year-old man after a dog bite (13,17,18). Other cases included a leg abscess in a 10-year-old after a dog bite and tenosynovitis in a 35-year-old healthy man after a Siberian tiger bite (12,20). The tenosynovitis case was polymicrobial in nature and also had a Comamonas species, P. mul- VOL.…”

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confidence: 99%

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Isolation of a Fastidious Bergeyella Species Associated with Cellulitis after a Cat Bite and a Phylogenetic Comparison with Bergeyella zoohelcum Strains

et al. 2004

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Bergeyella zoohelcum is an uncommon zoonotic pathogen typically associated with cat or dog bites. Previously, only five cases of B. zoohelcum infection have been reported. We report the isolation and characterization of a fastidious Bergeyella species from acute cellulitis in the upper extremity of a 60-year-old woman. The organism was too fastidious for identification and susceptibility testing with traditional culture methods. The isolate was characterized further by PCR amplification and sequencing of the 16S rRNA gene with broad-range eubacterial primers. Phylogenetic analysis of the 16S ribosomal DNA sequence indicated that this isolate was a member of the species B. zoohelcum (previously Weeksella zoohelcum), a gram-negative bacillus that is rarely associated with infections in humans. Despite sharing a close genetic relationship with other B. zoohelcum strains, this isolate was extremely fastidious in nature, raising the possibility that similar strains from cat or dog bite wound infections have been underreported.

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“…The oral cavities of many feline species are colonized with Pasteurella multocida, a nonmotile, gram-negative bacteria that can cause wound infection upon inoculation. 14,15 Less commonly, Neisseria weaveri species have also been cultured from the wounds of tiger bites. 5 In addition, bite wounds can be coinfected by Staphylococcus aureus from the victim's skin flora and by other anaerobic organisms from the animal's oral flora.…”

Section: Infection Prophylaxis After a Tiger Bitementioning

confidence: 99%

Traumatic tiger attack

Chum¹,

2011

PED

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Attacks on humans by large cats are uncommon occurrences and thus the principles of managing such injuries are not well documented. The authors here report the case of an 11-year-old boy who was mauled by a privately owned tiger. The attack resulted in multiple cranial lacerations and fractures, dissection of the internal carotid artery, and persistent neurological deficits. This case outlines the multiple sources of injury and pathology that can result from such an attack. Discussion is focused on the pattern of injury seen in large feline attacks and the treatment approach.

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Polymicrobial tenosynovitis withPasteurella multocidaand other Gram negative bacilli after a Siberian tiger bite

Cited by 43 publications

References 9 publications

Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds

Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds

Isolation of a Fastidious Bergeyella Species Associated with Cellulitis after a Cat Bite and a Phylogenetic Comparison with Bergeyella zoohelcum Strains

Traumatic tiger attack

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