Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China

Hao, Xiao-Fang; Lu, Zengjun; Kuang, Wendong; Sun, Pu; Fu, Yu; Wu, Lei; Zhao, Qing; Bao, Huang; Fu, Yuanfang; Cao, Yimei; Li, Pinghua; Bai, Xingwen; Li, Dong; Liu, Zaixin

doi:10.1186/1743-422x-8-73

Cited by 12 publications

(12 citation statements)

References 43 publications

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“…In 2006, an atypical PRRS outbreak in China was caused by an emerging highly-pathogenic PRRSV (HP-PRRSV) strain with a discontinuous deletion of 30-amino acid in the Nsp2 coding region in comparison with classical PRRSV strains [9,10]. Since then, the HP-PRRSV strain, which caused a more severe pneumonia and higher mortality than the classical PRRSV, has become the dominant strain found in China [11,12]. The immense genetic variability in the coding region of Nsp2 suggests that Nsp2 may play a limited role in viral replication but likely to play a very important part in modulating virus pathogenesis and the inflammation response in pigs during infection [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation

Fāng

Fang

Wang

et al. 2012

Virol J

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BackgroundNuclear factor-kappaB (NF-κB) is an inducible transcription factor that plays a key role in inflammation and immune responses, as well as in the regulation of cell proliferation and survival. Previous studies by our group and others have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection could activate NF-κB in MARC-145 cells and alveolar macrophages. The nucleocapsid (N) protein was identified as an NF-κB activator among the structural proteins encoded by PRRSV; however, it remains unclear whether the nonstructural proteins (Nsps) contribute to NF-κB activation. In this study, we identified which Nsps can activate NF-κB and investigated the potential mechanism(s) by which they act.ResultsBy screening the individual Nsps of PRRSV strain WUH3, Nsp2 exhibited great potential to activate NF-κB in MARC-145 and HeLa cells. Overexpression of Nsp2 induced IκBα degradation and nuclear translocation of NF-κB. Furthermore, Nsp2 also induced NF-κB-dependent inflammatory factors, including interleukin (IL)-6, IL-8, COX-2, and RANTES. Compared with the Nsp2 of the classical PRRSV strain, the Nsp2 of highly pathogenic PRRSV (HP-PRRSV) strains that possess a 30 amino acid (aa) deletion in Nsp2 displayed greater NF-κB activation. However, the 30-aa deletion was demonstrated to not be associated with NF-κB activation. Further functional domain analyses revealed that the hypervariable region (HV) of Nsp2 was essential for NF-κB activation.ConclusionsTaken together, these data indicate that PRRSV Nsp2 is a multifunctional protein participating in the modulation of host inflammatory response, which suggests an important role of Nsp2 in pathogenesis and disease outcomes.

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Section: Introductionmentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation

Fāng

Fang

Wang

et al. 2012

Virol J

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“…1, analysis of the complete deduced amino acid sequence of N-protein from the 36 PRRSV isolates shared common six mutations with Chinese HP-PRRSV strains. These mutations were also detected in other HP-PRRSV strains isolated in China [11,12] . Compared to classical strain CH-1a, four mutations at position 46, 91, 109 and 117 were just observed in HP strains.…”

Section: Discussionmentioning

confidence: 60%

“…N protein contains conserved nucleotide determinants such as nuclear localization signal (NLS), nuclear export signal (NES). Previous study had demonstrated that mutations at 43 and 44 within the NLS weaken viral replication [24] , NLS motif such as "Pat7" located at position 41-47 (PGKKNKK) might block the recognition of the epitopes [11] . In our study, the substitution K46R observed in 36 isolates and also in Chinese HP-PRRSV strains might influence to its function.…”

Section: Discussionmentioning

confidence: 99%

“…2), the 36 Vietnamese PRRSV isolates were belonged to the type II (NA genotype) and were grouped closely to each others and to representative Chinese HP strains, such as HUN4, JXA1, 09HEN2, 09HUB1,... (Lineage 1). They were divided into two subgroups, which is corresponding to subgroup IV and V of the previous classification [11] . The first subgroup contained 24 of our isolates belonged to eight haplotypes (2,4,5,8,9,10,11,12) and two Chinese HP reference strains (09HEN2 and 09HUB1).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…Those HP-PRRSV isolates share high sequence identity and have similar deletions/mutations in difference regions of viral genome such as two deletions in non-structural protein 2, one deletion in the 5'-untranslated region, and one deletion in the 3' untranslated region, and some other point mutations [9] . Recently, several studies on genetic variation and phylogenetic relationship based on major structural genes of PRRSV isolates such as ORF5, ORF7 have been done [10][11][12][13][14] . In this study, the 372-bp complete ORF7 of 36 PRRSV isolated from Vietnam collected during 2008-2012 were sequenced and analyzed with ORF7 sequences of PRRS vaccine viruses as well as its parental virus and other published PRRSV strains.…”

Section: Introductionmentioning

confidence: 99%

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Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Thúy¹,

Thu²,

Son³

et al. 2015

Kafkas Univ Vet Fak Derg

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Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases for the swine industry worldwide. The 372-bp complete nuclear capsid protein (N-protein) encoding gene (ORF7) of 36 field PRRSV isolates from Vietnam collected during 2008-2012 were sequenced and compared with certain vaccines and published strains of PRRSV. The ORF7 nucleotide sequence (nt) similarity and amino acid (aa) identity among 36 strains showed the highest and ranged from 95.1 to 100%. These isolates shared similarities with VR-2332 (nt 91.9-94.3%, aa 92.6-96.7%) and LV (nt 62.7-65.3%, aa 58.5-60.1%). There were higher level of similarity with QN07 (nt 96.2-99.1%, aa 96.7-99.1%) from the 2007 PRRS outbreak in Quang Nam province, CH-1a (nt 93.0-96.2%, aa 92.6-95.9%) isolated in China in 1995 and JXA1 (nt 96.7-99.7%, aa 97.5-100%), the highly pathogenic strain from China isolated in 2006. Six aa mutations located in both variable and conserved regions of N-protein amino acid sequence were detected in most of the 36 isolates and highly pathogenic PRRSV strains in China in comparison to the prototype strain VR-2332. Results of sequence analyses indicated that PRRSVs isolated in Vietnam during 2008-2012 were classified as North American genotype. Phylogenetic tree also clustered those 36 PRRSV isolates, other recently reported Vietnamese strains, highly pathogenic Chinese strains and JXA1-R vaccine strain in the same cluster and separated from the prototype strain (VR-2332) and Ingelvac MLV/Besta vaccine strains. The result on genetic characterization of ORF7 of circulating PRRSV strains may assist the development of effective strategies for monitoring and controlling PRRS in Vietnam. Keywords: Genetic variation of ORF7, North American genotype, Phylogenetic analysis, PRRSV Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni'nin Genetik Analizi

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Analysis of molecular variation of porcine reproductive and respiratory syndrome virus in Central China from 2006 to 2012

Shi

Xiong

et al. 2012

Arch Virol

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To analyze the epidemiology of PRRSV in Hubei Province of China, 668 serum samples collected from 14 pig-breeding farms were tested. We found that the PRRSV-positive rate was 5.24 % and that HP-PRRSV had become the dominant strain. To further investigate the genetic variation of PRRSV strains in this region, the complete gene sequences of nsp2, orf5, and orf7 from nine PRRSV strains collected during 2011-2012 were determined and compared with 33 known sequences. The results revealed that diverse HP-PRRSV strains are present in this region. An analysis of orf5 gene sequences showed that the strains collected during 2009-2010 formed a tightly clustered branch. When compared with the JXA1 strain, they had one mutation (V29 → A29) in a decoy epitope. Furthermore, we found that the number of potential N-glycosylation sites had apparently increased since 2006. These findings increase our knowledge of PRRSV epidemiology in Central China.

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Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China

Cited by 12 publications

References 43 publications

Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation

Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Analysis of molecular variation of porcine reproductive and respiratory syndrome virus in Central China from 2006 to 2012

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