Polymorphic microsatellite DNA markers in the endangered land snail, <i>Trichia caelata</i> (Gastropoda, Stylommatophora)

Armbruster, Georg F. J.; Alvarez, C.; Pesaro, M.; Baur, Bruno

doi:10.1111/j.1471-8286.2007.01801.x

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…DNA concentration was measured with a NanoDrop Ò ND-1000 UV-Vis Spectrophotometer (Thermo Fisher Scientific) and the DNA samples were diluted to a standard concentration of 10 ng/ll. Eight microsatellite loci (primers 08, 09, 10, 13, 16, 18, 22 and 26) developed for T. caelatus were used (Armbruster et al 2007b). PCR was performed in 25 ll volume following the protocol described by Armbruster et al (2007b).…”

Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

“…Eight microsatellite loci (primers 08, 09, 10, 13, 16, 18, 22 and 26) developed for T. caelatus were used (Armbruster et al 2007b). PCR was performed in 25 ll volume following the protocol described by Armbruster et al (2007b). Spreadex EL-400 or EL-600 gels (Elchrom Scientific AG, Switzerland) were used to resolve allelic patterns by horizontal electrophoresis performed on SEA 2000 advanced submerged gel electrophoresis equipment using M3 size marker (Elchrom Scientific AG, Switzerland; see Armbruster et al 2005).…”

Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

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High population differentiation in the rock-dwelling land snail (Trochulus caelatus) endemic to the Swiss Jura Mountains

et al. 2009

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Understanding patterns of genetic structure is fundamental for developing successful management programmes for isolated populations of threatened species. Trochulus caelatus is a small terrestrial snail endemic to calcareous rock cliffs in the Northwestern Swiss Jura Mountains. Eight microsatellite loci were used to assess the effect of habitat isolation on genetic population structure and gene flow among nine populations occurring on distinct cliffs. We found a high genetic differentiation among populations (mean F ST = 0.254) indicating that the populations are strongly isolated. Both allelic richness and effective population size were positively correlated with the size of the cliffs. Our findings support the hypothesis that T. caelatus survived on ice-free cliffs during the Pleistocene glacier advancements from the Alps. Due to the establishment of beech and pine forest under recent, temperate climate conditions, dispersal between cliffs is no longer possible for rock-dwelling snails such as T. caelatus. Our results provide basic data for developing a conservation action plan for this endangered gastropod species.

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Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

High population differentiation in the rock-dwelling land snail (Trochulus caelatus) endemic to the Swiss Jura Mountains

et al. 2009

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“…However, checking the results per locus in all populations, reveal only in two cases a significant deviation from HWE over two (Mont_5483) or even all three populations (Mont_4477). The high deficiency of heterozygotes over several loci has already been reported in other gastropod species and could be due to self-fertilization or the patchy distribution leading to drift effects due to restricted gene flow, single loci could be affected by allele scoring artefacts or the presence of null alleles [10]. Thus, the potential presence of null alleles was tested using MICRO-CHECKER 2.2.3 [11].…”

Section: Resultsmentioning

confidence: 99%

First establishment of microsatellite markers in clausiliid snails (Mollusca: Gastropoda: Clausiliidae)

et al. 2017

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BackgroundClausiliidae (door snails) are gastropods with a very high diversity concerning shell morphology, especially of their complex closing apparatus, which provides the most important diagnostic traits for classification of taxa. Due to the high variability, a high number of taxa has been described, though their systematics and taxonomy is partially controversially discussed. Montenegrina is the second most speciose door snail genus in Europe. It is an obligate rock-dwelling land snail and has, compared to its complex systematics, a rather small distribution range in the western parts of the Balkan Peninsula. The different taxa themselves show a very narrow and patchy distribution range. As Montenegrina is comprehensively sampled over the whole distribution range, it is a perfect study system for general questions on speciation and morphological differentiation in land snails. To study the amount of gene flow between geographically close or co-occurring populations, highly polymorphic markers are needed.ResultsThirteen microsatellite loci with a tetranucleotid repeat were isolated and tested in three geographically close Montenegrina populations (two populations of M. dofleini prespaensis from the Prespa Lake, n = 35 and one population from M. stankovici from the Ohrid Lake, n = 20). The number of alleles per locus ranged from 2 to 27. No significant linkage disequilibria between the same two loci were found in all three tested populations. The deviation from Hardy–Weinberg equilibrium reveal only for two loci a significant deviation from HWE in more than one population (Mont_5483 and Mont_4477).ConclusionThe 13 newly established genetic markers will help to gain better insights to the population genetic structure of Montenegrina and might reveal new results about speciation processes in co-occurring taxa. Furthermore, these microsatellite loci could also be tested in other clausiliid species.

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“…Plasmids from 46 positive clones were sequenced and primers were designed for 12 microsatellite inserts, all of which were tested for polymorphism. ecogenics GmbH used M13‐modified primers to determine the polymorphism of the microsatellite markers (for details see Schuelke 2000 and Armbruster et al . 2007).…”

Section: Cross‐amplification Of Grass Snake (Natrix Natrix) Microsatementioning

confidence: 99%

Novel microsatellite loci in the grass snake (Natrix natrix) and cross‐amplification in the dice snake (Natrix tessellata)

Meister¹,

Armbruster²,

Frauenfelder³

et al. 2009

Molecular Ecology Resources

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Six novel polymorphic microsatellite loci are presented for the grass snake (Natrix natrix), a species with declining populations in many regions. The number of alleles per locus ranged from two to seven. Four dice snake (Natrix tessellata) microsatellites were polymorphic in the grass snake with three to four alleles. At two loci, the expected heterozygosity differed significantly from observed heterozygosity. Cross-amplification of the grass snake markers in the dice snake showed two polymorphic microsatellites with two and four alleles.

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Polymorphic microsatellite DNA markers in the endangered land snail, Trichia caelata (Gastropoda, Stylommatophora)

Cited by 3 publications

References 10 publications

High population differentiation in the rock-dwelling land snail (Trochulus caelatus) endemic to the Swiss Jura Mountains

High population differentiation in the rock-dwelling land snail (Trochulus caelatus) endemic to the Swiss Jura Mountains

First establishment of microsatellite markers in clausiliid snails (Mollusca: Gastropoda: Clausiliidae)

Novel microsatellite loci in the grass snake (Natrix natrix) and cross‐amplification in the dice snake (Natrix tessellata)

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