Polymorphic microsatellite loci for eusocial wasps (Hymenoptera: Vespidae)

Daly, Derek; Archer, M; Watts, Phillip C.; Speed, Michael P.; Hughes, Martin; Barker, F. S.; Jones, J.; Odgaard, K.; Kemp, Stephen J.

doi:10.1046/j.1471-8286.2002.00220.x-i2

Cited by 34 publications

(12 citation statements)

References 3 publications

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“…Along with these males, a queen and multiple workers were sampled for evaluation of the quality of male DNA, and to assure the haploid origin of the males. The quality of the DNA originating from different body parts (head, thorax, abdomen) of a few individuals was tested based on amplification using three microsatellite primer pairs (LIST 2001 F-R, LIST 2002 F-R and LIST 2003 F-R [38]). PCR-conditions were optimized for each microsatellite primer pair as follows: For LIST 2001 we used an annealing temperature of 52°C and 1.5 mM MgCl 2 , for LIST 2002 an annealing temperature of 47°C and 1.5 mM MgCl 2 and for LIST 2003 an annealing temperature of 54°C and 1.0 mM MgCl 2 .…”

Section: Methodsmentioning

confidence: 99%

A high recombination rate in eusocial Hymenoptera: evidence from the common wasp Vespula vulgaris

Sirviö

Johnston

Wenseleers³

et al. 2011

BMC Genet

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BackgroundHigh recombination rates have previously been detected in two groups of eusocial insects; honeybees and ants. In this study we estimate recombination rate in a eusocial wasp Vespula vulgaris that represents a third phylogenetic lineage within eusocial hymenopterans.ResultsA genetic linkage map of V. vulgaris based on 210 markers shows that the total map length is 2129 cM and the recombination rate is 9.7 cM/Mb (or 103 kb/cM). The present estimate in V. vulgaris is somewhat smaller than in the honeybee Apis mellifera and intermediate between the estimates from two ant species (Acromyrmex echinatior, Pogonomyrmex rugosus). Altogether, the estimates from these eusocial species are higher than in any other insect reported so far.ConlusionsThe four species (V. vulgaris, A. mellifera, A. echinatior, P. rugosus) are characterized by advanced eusociality with large colonies, clear queen-worker dimorphism and well developed task specialization. They also have colonies with a single, normally multiply inseminated (polyandrous) queen. Benefits of genotypic diversity within colonies (e.g. through improved task specialization or pathogen and parasite resistance) may have selected for both polyandry and high recombination rate in such advanced eusocial insects.

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Section: Methodsmentioning

confidence: 99%

A high recombination rate in eusocial Hymenoptera: evidence from the common wasp Vespula vulgaris

Sirviö

Johnston

Wenseleers³

et al. 2011

BMC Genet

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“…For monogynous colonies, DNA microsatellite genotyping has revealed a large variation in queen mating frequency from one to 69 mates per queen (Boomsma & Ratnieks 1996; Crozier & Pamilo 1996; Strassmann 2001; Tarpy & Nielsen 2002). Although DNA microsatellite markers have been developed for the Vespinae (Thorén 1998; Daly et al . 2002; Hasegawa & Takahashi 2002), only a small number of markers are available, so that data on paternity distribution remain limited (Strassmann 2001).…”

Section: Introductionmentioning

confidence: 99%

Mating frequency and genetic relatedness of workers in the hornetVespa analis(Hymenoptera: Vespidae)

Takahashi

Akimoto

Nakamura

et al. 2003

Entomological Science

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Mating frequency of Vespa analis queens and the genetic relatedness of their workers was analyzed by DNA microsatellite genotyping. Of 20 colonies studied, 18 had a queen inseminated by a single male and two had queens each inseminated by two males. The estimated effective number of matings was 1.05 ± 0.037 (mean ± SE), with 75–85% of the offspring of the two multiply mated queens sired by a single male. The pedigree relatedness between nestmate workers averaged over the 20 colonies was estimated to be 0.74 ± 0.008, almost identical to the predicted value of 0.75 for colonies headed by a singly mated queen. Multiple matrilines; that is, the presence of workers not related to the current queens, were detected in six colonies, suggesting that queen replacement occurred via usurpation of the founding queens in these six colonies. These results demonstrate that the kin structure of V. analis is similar to that reported in other vespid species.

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“…Genotypes of V. velutina populations were assessed using 11 of the 15 microsatellite loci previously developed for the analysis of the origin of the invasion of V. velutina in France: D2-185, R4-100, R4-114, D3-15, R1-36, R1-75, R1-77, R4-33, R1-137, R1-169 and D2-142 [36], as well as two supplementary loci (List 2015 and List 2020B) [37]. PCR protocols and genotype scoring are detailed by Arca [38].…”

Section: Methodsmentioning

confidence: 99%

Geographic Variation of Melanisation Patterns in a Hornet Species: Genetic Differences, Climatic Pressures or Aposematic Constraints?

et al. 2014

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Coloration of stinging insects is often based on contrasted patterns of light and black pigmentations as a warning signal to predators. However, in many social wasp species, geographic variation drastically modifies this signal through melanic polymorphism potentially driven by different selective pressures. To date, surprisingly little is known about the geographic variation of coloration of social wasps in relation to aposematism and melanism and to genetic and developmental constraints. The main objectives of this study are to improve the description of the colour variation within a social wasp species and to determine which factors are driving this variation. Therefore, we explored the evolutionary history of a polymorphic hornet, Vespa velutina Lepeletier, 1836, using mitochondrial and microsatellite markers, and we analysed its melanic variation using a colour space based on a description of body parts coloration. We found two main lineages within the species and confirmed the previous synonymy of V. auraria Smith, 1852, under V. velutina, differing only by the coloration. We also found that the melanic variation of most body parts was positively correlated, with some segments forming potential colour modules. Finally, we showed that the variation of coloration between populations was not related to their molecular, geographic or climatic differences. Our observations suggest that the coloration patterns of hornets and their geographic variations are determined by genes with an influence of developmental constraints. Our results also highlight that Vespa velutina populations have experienced several convergent evolutions of the coloration, more likely influenced by constraints on aposematism and Müllerian mimicry than by abiotic pressures on melanism.

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Polymorphic microsatellite loci for eusocial wasps (Hymenoptera: Vespidae)

Cited by 34 publications

References 3 publications

A high recombination rate in eusocial Hymenoptera: evidence from the common wasp Vespula vulgaris

A high recombination rate in eusocial Hymenoptera: evidence from the common wasp Vespula vulgaris

Mating frequency and genetic relatedness of workers in the hornetVespa analis(Hymenoptera: Vespidae)

Geographic Variation of Melanisation Patterns in a Hornet Species: Genetic Differences, Climatic Pressures or Aposematic Constraints?

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