Polymorphic microsatellite loci isolated from the great scallop, Pecten maximus (Bivalvia: Pectinidae)

Abstract

We isolated and characterized nine polymorphic microsatellite loci from the great scallop (Pecten maximus) as part of an attempt to better understand the population–genetic structure of this species. Levels of genetic diversity were assessed in 32 scallops collected from the coastal waters of Port Erin Bay, Isle of Man. The number of alleles ranged from two to 29 and observed and expected heterozygosities varied between 0.031 to 0.906 and 0.062 to 0.973, respectively. Two loci showed significant (P < 0.05) heterozygote deficits, likely because of null (nonamplifying) alleles; one pair of loci was in linkage disequilibrium.

Multiplex PCR sets of novel microsatellite loci for the great scallopPecten maximusand their application in parentage assignment

“…The new microsatellites display the same range of variability as those described previously in P. maximus by Watts et al (2005), which were also isolated from genomic DNA. In contrast, the present markers are more polymorphic than those identified by Charrier et al (2012) that were derived from expressed sequence tags (Na varying from 2 to 11 alleles, and H o from 0.05 to 0.84).…”

“…Null allele frequency estimates were computed using CERVUS 3.0. The eight best quality loci were selected for the multiplexing process, in addition to three loci from the literature: LIST-15_005 and LIST-15_012 (Watts et al 2005) and PmGC05 (Charrier et al 2012). …”

“…They are especially useful for the characterization of genetic stocks, parentage analysis and broodstock selection, linkage mapping, and studies of a Corresponding author: jean.laroche@univ-brest.fr quantitative trait loci (Chistiakov et al 2006). However, few microsatellite markers have been described so far for P. maximus (Watts et al 2005;Charrier et al 2012).…”

Isolation and characterization of microsatellite markers in the queen scallopAequipecten opercularisand their application to a population genetic study

“…The number of polymorphic microsatellite loci obtained with respect to the total sequences examined in A. opercularis (6.0%) is higher than that yielded by enriched libraries constructed in the scallop Mizuhopecten yessoensis (4.6%, An et al 2005), the oyster Crassostrea virginica (2.4%, Reece et al 2004) or the pearl oyster Pinctada maxima (0.2%, Evans et al 2006), and lower than that obtained in other scallops, Chlamys farreri (12.5%, Zhan et al 2007), Pecten maximus (15.8%, Watts et al 2005) and C. nobilis (27.5%, Ma and Yu 2009) or the oyster C. gigas (19.6%, Li et al 2003). The efficiency of the isolation of microsatellites as usable markers might be affected by both biological and technical factors.…”

Identification, Inheritance, and Variation of Microsatellite Markers in the Black Scallop Mimachlamys varia

“…In other scallop species, such as Mizuhopecten yessoensis and Aequipecten opercularis (Arias et al 2010), minisatellites have also been found in clones with microsatellites, but in these cases they are near or surrounding the microsatellite. The number of polymorphic microsatellite loci obtained with respect to the total sequences examined in Mimachlamys varia (5.62%) was within the range observed for other species where enriched libraries were examined; values obtained in other species were 4.55% in Mizuhopecten yessoensis ), 6.02% in A. opercularis (Arias et al 2010), and 15.79% in Pecten maximus (Watts et al 2005). The abundance of microsatellites and their location in the genomes may explain the differences observed among species in the success of establishing reliable microsatellite markers (Meglécz 2007).…”