Polymorphic microsatellite markers for the endangered golden-headed lion tamarin, Leontopithecus chrysomelas (Callitrichidae)

Galbusera, Peter; Gillemot, Sarah

doi:10.1007/s10592-007-9370-7

Cited by 16 publications

(35 citation statements)

References 7 publications

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“…Results found in this study showed similar estimates of genetic diversity with those found in captive GHLT populations [Galbusera and Gillemot, 2008;Orefice, 2015], even though the captive population is based on a relatively small number of founders (around 40). In addition, these similar genetic diversities among captive populations and our results were found even using different types of samples: skin (n = 29, loci = 9, N a = 3.67, H e = 0.59 [Galbusera and Gillemot, 2008]), blood (n = 55, loci = 16, N a = 5.1, H e = 0.65) and hair (n = 49, loci = 16, N a = 4.6, H e = 0.55) [Orefice, 2015]. The importance of genetically diverse captive populations for the conservation of lion tamarin species with markedly smaller wild populations has also been documented [Ayala-Burbano et al, 2017;Moraes et al, 2017].…”

Section: Genetic Diversitysupporting

confidence: 82%

“…DNA amplifications were performed in the thermocycler TC412 (Techn), and profiles of the amplification reactions can be seen in Table 1. Seven polymorphic loci isolated from L. chrysomelas were used -Lchµ1, Lchµ3, Lchµ4, Lchµ5, Lchµ6, Lchµ8 and Lchµ9 [Galbusera and Gillemot, 2008]. These primers were constructed with M13 tails and used in combination with an M13 primer that had the complementary sequence but was dye-labelled, following the protocol established by Schuelke [2000].…”

Section: Laboratory Proceduresmentioning

confidence: 99%

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Population Genetic Structure of an Endangered Endemic Primate (Leontopithecus chrysomelas) in a Highly Fragmented Atlantic Coastal Rain Forest

Moraes

Grativol

Vleeschouwer

et al. 2018

IJFP

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This study evaluated the genetic structure of wild populations of the endangered primate, Leontopithecus chrysomelas. We tested the assumption that populations of L. chrysomelas, given their larger population size and a higher degree of habitat continuity, would have higher genetic diversity and less genetic structuring than other lion tamarins. We used 11 microsatellites and 122 hair samples from different locations to assess their genetic diversity and genetic structure, and to make inferences about the isolation by distance. The overall expected heterozygosity (0.51 ± 0.03) and the average number of alleles (3.6 ± 0.2) were relatively low, as is the case in other endangered lion tamarins. Genetic clustering analyses indicated two main clusters, whereas the statistical analyses based on genotype similarities and F_st suggested further substructure. A Mantel test showed that only 34% of this genetic differentiation was explained by the linear distance. In addition to linear distance, structural differences in the landscape, physical barriers and behavioural factors may be causing significant genetic structuring. Overall, this study suggests that these populations have a relatively low genetic diversity and a relatively high population genetic structure, putting in question whether the presence of agroforest systems (known locally as cabruca) is enough to fully re-establish functional landscape connectivity.

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Section: Genetic Diversitysupporting

confidence: 82%

Section: Laboratory Proceduresmentioning

confidence: 99%

Population Genetic Structure of an Endangered Endemic Primate (Leontopithecus chrysomelas) in a Highly Fragmented Atlantic Coastal Rain Forest

Moraes

Grativol

Vleeschouwer

et al. 2018

IJFP

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“…From these 19 microsatellites, 17 (89.4 %) pro-duced robust alleles, of which 9 (47.4%) were polymorphic and 8 (42.1%) monomorphic; the remaining 2 (10.5%) failed to amplify fragments under all tested conditions [Martins and Galetti Jr., 2011]. As such, the 9 polymorphic microsatellite markers used by us were those identified in L. rosalia [Grativol et al, 2001], L. chrysopygus [Perez-Sweeney et al, 2005] and L. chrysomelas [Galbusera and Gillemot, 2008]. One primer of each locus pair was constructed with a M13 tail on the 5 -end, and a fluorescently labelled M13 primer was used in a 3-primer PCR, following a pre-established protocol [Schuelke, 2000].…”

Section: Microsatellite Genotypingmentioning

confidence: 81%

Genetic Analysis Reveals Population Structuring and a Bottleneck in the Black-Faced Lion Tamarin (Leontopithecus caissara)

Martins

Nascimento²,

Nali³

et al. 2012

IJFP

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The ability of a population to evolve in a changing environment may be compromised by human-imposed barriers to gene flow. We investigated the population structure and the possible occurrence of a genetic bottleneck in two isolated populations of the black-faced lion tamarin (Leontopithecus caissara), a species with very reduced numbers (less than 400) in a very restricted range in the Atlantic Forest of southeast Brazil. We determined the genotypes of 52 individuals across 9 microsatellite loci. We found genetic divergence between the populations, each exhibiting low genetic diversity. Analysis revealed broad- and fine-scale population structuring. Both populations have evidently experienced population reduction and a genetic bottleneck without presenting any apparent detrimental effect. Anyway, measures should be taken to effectively protect the forests where L. caissara occurs in order to allow its populations to increase and counteract the eventual effects of genetic impoverishment.

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“…*Departs significantly from HWE at P = 0.017 after correction (Benjamini and Yekutieli, 2001). d Galbusera and Gillemot (2008).…”

Section: Resultsmentioning

confidence: 99%

“…Seventeen microsatellites, which were previously designed for and successfully amplified in multiple Neotropical primate species, were tested. Four microsatellites were isolated in at least two Sapajus species (PEPC3, PEPC8, PEPC40, and PEPC59: Escobar-Páramo, 2000), one in Lagothrix lagotricha (PEPL4: Escobar-Páramo, 2000), eight in Cebus capucinus (Ceb3, Ceb8, Ceb9, Ceb11, Ceb119, Ceb120, Ceb121, and Ceb130: Muniz and Vigilant, 2008), one in Leontopithecus rosalia (Lr.P2BH6: Grativol et al, 2001), two in Leontopithecus chrysopygus (Leon15c85 and Leon21c75: Perez-Sweeney et al, 2005), and one in Leontopithecus chrysomelas (Lchµ07: Galbusera and Gillemot, 2008).…”

Section: Methodsmentioning

confidence: 98%

Transferability of microsatellites for studies on the social behavior of the tufted capuchin monkey (genus Sapajus)

Tokuda¹,

Martins²,

Izar³

2014

Genet. Mol. Res.

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ABSTRACT. Because of relevant results that indicated that molecular techniques can provide increased knowledge of animal social systems, they usually complement observational field studies. Despite the great utility of microsatellites, they are not available for all species. Gathering genetic information using microsatellites that were originally designed for other species is a time-saving procedure. The aim of this study was to test the transferability of microsatellites and their usefulness in studies of social behavior of black capuchin monkeys (Sapajus nigritus). We noninvasively sampled adult and subadult black capuchins of three wild groups in southeastern Brazil. Seventeen microsatellites, which were previously designed for and successfully amplified in multiple Neotropical primate species, were tested. Nine of the 17 microsatellite loci tested produced an average of 6.22 alleles (range 2-12) per locus. The allelic richness and the expected heterozygosity for all loci was 5.93 and 0.70, respectively. The combined non-exclusion probability for one candidate parent across all loci was 0.01. The nine microsatellite loci Microsatellites for capuchin monkeys optimized in this study have a great potential for application in studies of social structure and dispersal patterns in S. nigritus populations and in other Neotropical primate species.

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Polymorphic microsatellite markers for the endangered golden-headed lion tamarin, Leontopithecus chrysomelas (Callitrichidae)

Cited by 16 publications

References 7 publications

Population Genetic Structure of an Endangered Endemic Primate (Leontopithecus chrysomelas) in a Highly Fragmented Atlantic Coastal Rain Forest

Population Genetic Structure of an Endangered Endemic Primate (Leontopithecus chrysomelas) in a Highly Fragmented Atlantic Coastal Rain Forest

Genetic Analysis Reveals Population Structuring and a Bottleneck in the Black-Faced Lion Tamarin (Leontopithecus caissara)

Transferability of microsatellites for studies on the social behavior of the tufted capuchin monkey (genus Sapajus)

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