Polymorphism analysis of three Chinese indigenous sheep breeds by microsatellite markers

Bai, Jun; Yang, You; Wang, Yu Qin; Zhang, Xiao Hui; Pang, You Zhi

doi:10.18805/ijar.5566

Cited by 9 publications

(10 citation statements)

References 11 publications

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“…Nei's genetic distances (Nei, 1972) between the five populations of sheep were calculated using 12 STR loci (Table 3), which varied from 0.469 to 0.217. The results of genetic distance of the present study were lower than the findings of Bai et al (2015), who reported that the genetic distance ranged from 0.21 to 0.62 for Chinese indigenous sheep breeds, which was higher than that of Egyptian sheep breeds (Rushdi et al, 2015). The values of genetic distances showed that the investigated sheep populations are characterized as having a high range of variability of the allele pool, presence or absence of certain alleles, and differences in frequency of occurrence alleles.…”

Section: Discussioncontrasting

confidence: 94%

“…Today, the FAO and the ISAG-FAO Advisory Group on Animal Genetic Diversity recommend specific sets of STR (short tandem repeat, also known as microsatellite) loci for genetic analyses, such as for horse, cattle, and pig breeds. Of the different types of molecular markers, STRs are suitable for studying genetic diversity because of their abundancethe large amount of allelic variation at each locus is highly polymorphic, their distribution throughout the genome is random, and inheritance is codominant (Rekha et al, 2016;Barcaccia et al, 2013;Putman et al, 2014). In addition, STRs are able to generate information for the planning of crossings and further selection of genotypes in genetic breeding programs (Faleiro et al, 2007;Crispim et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of different breeds of Kazakh sheep using microsatellite analysis

et al. 2019

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A total of 75 individuals from five sheep populations in Kazakhstan were investigated based on 12 STR (short tandem repeat, also known as microsatellite) markers in order to study their genetic structure and phylogenetic relationship based on genetic distances. These sheep had a high level of genetic diversity. In total, 163 alleles were found in all the populations using 12 microsatellite loci. The mean number of alleles, effective number of alleles, and polymorphism information content (PIC) values per loci were 13.4, 5.9, and 0.78, respectively. Comparing the allelic diversity between the populations, the highest genetic diversity was observed in the Edilbay-1 sheep breed (8.333±0.644), and the lowest parameter was for Kazakh Arkhar-Merino (7.083±0.633). In all populations, there is a deficiency of heterozygosity. The largest genetic diversity was found in loci INRA023 and CSRD247 with 16 alleles, and the smallest polymorphism was noted for the locus D5S2 with 8 alleles. The level of observed heterozygosity was in the range 0.678±0.051 for Kazakh Arkhar-Merino and 0.767±0.047 for Kazakh fat-tailed coarse wool. The expected heterozygosity level range was from 0.702±0.033 for Kazakh Arkhar-Merino to 0.777±0.023 for Edilbay-1. When 12 microsatellite loci are compared, the OarFCB20 locus showed the highest level of genetic variability. Excess of heterozygosity was observed at three loci; MAF065, McM042, and OarFCB20. The highest genetic distance was observed between Kazakh Arkhar-Merino and Edilbay-1, whereas the genetic distance between Edilbay-1 and Edilbay-2 is the smallest using Nei's standard genetic distance. The Edilbay-1 sheep breed possesses the largest genetic diversity among these five populations.

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Section: Discussioncontrasting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of different breeds of Kazakh sheep using microsatellite analysis

et al. 2019

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“…When Table 1 was analyzed in terms of PIC value, it was observed that there was a difference among quail lines. This value could be stated as higher than 0.477 (Kayang et al, 2002), but lower than 0.57 (Chang et al, 2007), 0.64 (Farrag et al, 2011), and 0.69 (Bai et al, 2013). Also, Tadano et al (2014) reported that of 49 polymorphic microsatellite markers, 55.1% were highly informative (PIC≥0.50), 28.6% were reasonably informative (0.50>PIC>0.25) and 16.3% were slightly informative (PIC ≤0.25) according to Botstein et al (1980) criteria.…”

Section: Discussionmentioning

confidence: 86%

“…This value could be informative for such studies according to Farrag et al (2011) who suggested that the average number of alleles per locus in the genetic studies distances must be >4 to reduce the standard error in the estimation of genetic distances. Average allele number found in studies that were carried on different quail genotypes by Chang et al (2007), Farrag et al (2011), Tadano et al (2014) and Bai et al (2013) as 2.33 to 5.67, 5.39, 5.20, and 5.3, respectively. As clearly seen here we found a higher allele number.…”

Section: Discussionmentioning

confidence: 94%

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Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

Karabağ

Balcıoğlu

Karlı

et al. 2016

Asian Australas. J. Anim. Sci

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Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research was to determine genetic diversity and population genetic structures of selected Japanese quail lines (high body weight 1 [HBW1], HBW2, low body weight [LBW], and layer [L]) throughout 15th generations and an unselected control (C). A total of 69 individuals from five quail lines were genotyped by fifteen microsatellite markers. When analyzed profiles of the markers the observed (Ho) and expected (He) heterozygosity ranged from 0.04 (GUJ0027) to 0.64 (GUJ0087) and 0.21 (GUJ0027) to 0.84 (GUJ0037), respectively. Also, Ho and He were separated from 0.30 (L and LBW) to 0.33 (C and HBW2) and from 0.52 (HBW2) to 0.58 (L and LBW), respectively. The mean polymorphic information content (PIC) ranged from 0.46 (HBW2) to 0.52 (L). Approximately half of the markers were informative (PIC≥0.50). Genetic distances were calculated from 0.09 (HBW1 and HBW2) to 0.33 (C and L). Phylogenetic dendrogram showed that the quail lines were clearly defined by the microsatellite markers used here. Bayesian model-based clustering supported the results from the phylogenetic tree. These results reflect that the set of studied markers can be used effectively to capture the magnitude of genetic variability in selected Japanese quail lines. Also, to identify markers and alleles which are specific to the divergence lines, further generations of selection are required.

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Assessment of variations in productive performance of two different plumage color varieties of Japanese quail and their reciprocal crosses

Elkhaiat

El‐Kassas

Eid

et al. 2023

Trop Anim Health Prod

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This study aimed to detect the phenotypic differences between the brown (BB) and white (WW) feathered quails and their reciprocal crosses (BW and WB) over two successive generations. The WW and cross quails, especially the BW, had the heaviest body weights, throughout the studied period, with significant variations between the two studied generations (P<0.05). Moreover, the WW and BW possessed the largest egg production during the F1, while in the F2, the BB had superiority among the studied quails with a prominent superiority of the F2 over the F1 (P<0.05). However, the F1 had higher egg weights than F2 with superiority of WW quails compared to the others (P<0.05). Also, the WW quails had the lowest lipid contents of the eggs. These phenotypic variations among the studied quails might be preliminarily explained by the results of the analyzed microsatellite markers despite the few markers used. The high variability among the BW and WB quails might be due to the larger number of alleles (NA and Ne) and the lower values of FIS with low heterozygosity levels (HO and He). Moreover, the BW and BB were the closest, while WB and WW were the farthest because of the high and low genetic identities and the high and low genetic distance between them, respectively. So the obtained results might introduce an initial scientific basis for evaluating and employing the genetic properties of BB, WW, BW, and WB quails in further genetic improvement program, and more microsatellite markers are recommended.

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Polymorphism analysis of three Chinese indigenous sheep breeds by microsatellite markers

Cited by 9 publications

References 11 publications

Genetic diversity of different breeds of Kazakh sheep using microsatellite analysis

Genetic diversity of different breeds of Kazakh sheep using microsatellite analysis

Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

Assessment of variations in productive performance of two different plumage color varieties of Japanese quail and their reciprocal crosses

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