Polymorphism Identification of Pit1 Gene in Indonesian Buffaloes (Bubalus bubalis) and Holstein-Friesian Cows

Abstract

Pit-1 gene has been identifi ed as the pituitary specifi c transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) genes in the anterior pituitary. The use of polymorphic markers in breeding programmes could make selection more accurate and effi cient. A total of 320 Indonesian buff aloes from four populations and 45 FH cows from nine populations were genotyped for polymorphism of Pit1|Hinf1 gene by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. The gene polymorphism was detected only in FH ca le. The frequency of AA, AB, and BB genotype was 2%, 44%, and 53% respectively. The frequency of A allel was 25% and B allel was 75%, but no polymorphism was detected in 320 Indonesia buff aloes.

Associations of Leptin and Pituitary‐Specific Transcription Factor Genes’ Polymorphisms with Reproduction and Production Traits in Dairy Buffalo

“…Our results regarding Pit‐1 gene was supported by the finding of Misrianti et al. () and Othman et al. ().…”

“…Our results regarding Pit-1 gene was supported by the finding of Misrianti et al (2010) and Othman et al (2011). They reported that all studied animals exhibited monomorphic pattern in Pit1Hinf1 locus in Indonesian and Egyptian buffaloes; thus, the association between Pit-1 gene and productive and reproductive traits could not be achieved due to the monomorphism.…”

Identification of a Single Nucleotide Polymorphism at Hinf-1 Enzyme Restriction Site of Pit-1 Gene on Indonesian Bali Cattle Population

“…Besides, the study of this gene was also intensively conducted in native cattle breeds in some countries, including Iranian cattle (Javanmard et al, 2005), Southern Anatolian red cattle (Oztabak et al, 2008), Hanwoo cattle (Han et al, 2010;Seong et al, 2011), Turkey cattle (Ozdemir, 2012), Qinchuan cattle (Zhang et al, 2009) and Piemontese cattle (Ribeca et al, 2014). Even more, Pit-1 gene was also identified in non-cattle species, including chicken (Nie et al, 2008), sheep (Bastos et al, 2006) and buffalo (Javanmard et al, 2005;Misrianti et al, 2010).…”

The polymorphism in g.1256G>A of bovine pituitary specific transcription factor-1 (bPIT-1) gene and its association with body weight of Pasundan cattle

“…Previous studies reported that one SNP was in the exon 6 of bPit-1 gene at position g.1256G>A based on GenBank: Y15995 (Javanmard et al, 2005;Misrianti et al, 2010;Aytekin and Boztepe, 2013;Nahavandi et al, 2010;Chauhan et al, 2015;Bayram et al, 2017). Moreover, SNP of g.1256G>A can be detected by HinfI restriction enzyme through PCR-RFLP method (Dybus et al, 2003).…”