Polymorphism in miR-31 and miR-584 binding site in the angiotensinogen gene differentially influences body fat distribution in both sexes

Abstract

Angiotensinogen (AGT), its active fragments and microRNA-31 (miR-31) play an important role in adipocyte differentiation. AGT contains a miR-31 polymorphic binding site. We hypothesize that the rs7079 polymorphism in the miR-31/584 binding site of the AGT gene could influence body fat distribution. A total of 751 subjects (195 men, 556 women) were enrolled in the study. The rs7079 genotypes were determined by qRT-PCR. Anthropometric measurements were taken on all subjects, who were subsequently divided into two groups: obese ([30 kg m -2 ) and non-obese (\30 kg m -2 ). Linear regression models were created to determine the contributions of sex, obesity status and rs7079 to all measured parameters. Adding the rs7079 genotype significantly contributed to the linear regression model for waist circumference (p = 0.013), hip circumference (p = 0.018) and supraspinal skin-fold thickness (p = 1 9 10 -3 ). Differences between sexes and between the obese and nonobese groups were observed. Waist circumference was lower in men carrying the A allele (p = 0.022); hip circumference was higher only in obese women carrying the A allele (p = 0.015). While men carrying the A allele had lower supraspinal skin-fold thickness (p = 0.022), this parameter was found to be higher in A allele carrying women (p = 3 9 10 -3 ). The higher total sum of skin-fold thickness in A allele carrying women was restricted to obese individuals (p = 0.028). The presence of the A allele was associated with both lower tricipital skin-fold thickness in non-obese women (p = 0.023) and a trend of higher thickness in non-obese men (p = 0.065). Significant associations of rs7079 in the AGT gene and body fat distribution were observed. The distribution followed opposing patterns in both sexes.

Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness

“…Overexpression of miR-137 inhibited both human adipose tissue stromal cell proliferation and adipogenic differentiation by negatively controlling protein and mRNA levels of the cell division control protein 42 homolog (CDC42) [37]. MiR-31 has been shown to play an important role in the adipogenic differentiation process [38], and could influence body fat distribution by regulating the angiotensinogen (AGT) gene [39]. MiR-183, which was up-regulated in the HB group, was found to promote 3T3-L1 adipogenesis by inhibiting the Wnt/β-catenin signalling pathway [40].…”

High expression of microRNA‑31 and its host gene LOC554202 predict favorable outcomes in patients with colorectal cancer treated with oxaliplatin

“…Similarly, the contribution of miR-31 polymorphisms to the survival rates of patients with CRC cannot be ruled out and requires investigation in future investigations. To date, polymorphisms of miR-31 have been described in the involvement of this RNA in the regulation of angiotensinogen expression and body fat distribution (50). The miR-31 rs13283671 polymorphism was previously described in lung cancer, although it was not correlated with survival rate and/or certain clinicopathological parameters (51).…”

Differentially Expressed miRNA-Gene Targets Related to Intramuscular Fat in Musculus Longissimus Dorsi of Charolais × Holstein F2-Crossbred Bulls

“…Interestingly, miR-10b through targeting PPARA [ 55 ], miR-101 by modulating phosphatidylserine synthase 1 ( PTDSS1 ) and fatty acid elongase 5 ( ELOVL5 ) genes [ 56 ], miR-100 curtailing IGF1R and SREBF1 [ 57 , 58 ] and miR-135a regulating IRS2 play important roles in fat accretion, lipid and fatty acid metabolism and insulin signaling. While miR-135a [ 59 ] and miR-31 [ 60 , 61 ] are downregulated in 3T3L-1 cellular adipogenesis, they have also been associated with increased glycerol/cholesterol levels [ 62 ] and fat deposition through angiotensin [ 63 ], respectively. The expression of miR-874 has been confirmed and found increased in cattle with moderate intramuscular fat [ 24 ].…”