Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon

Machado, Edson; Souza, Bonifácio Benício de; Guimarães, R C; Azevedo, José Luís; Gonçalves, Evonnildo Costa; Ribeiro, H. F. L.; Filho, S. T. Rolim; Filho, Ednaldo da Silva

doi:10.4238/gmr.15027960

Cited by 3 publications

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References 14 publications

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“…Machado et al (2016) also observed inbreeding (based on a SNP found on an intron of the melatonin receptor gene) in two Amazonian populations of buffaloes that are characterized by their systems of rearing (Terra Firme and Varzea); the authors concluded that both populations had low to moderate genetic variability.…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

et al. 2016

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ABSTRACT. Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production. By using the polymerase chain reaction-single-strand conformation polymorphism technique, the alleles A (0.79), B (0.20), and D (0.01) were found in the Murrah breed. In the Mediterranean and mixed-breed buffaloes, we found .03), and the Mediterranean and mixed-breed buffaloes had the genotypes AA (0.44) and (0.61), AB (0.50) and (0.31), and BB (0.06) and (0.08), respectively. For the Murrah, Mediterranean, and mixedbreed buffaloes, respectively, the expected heterozygosity values were 0.34, 0.43, and 0.35, the inbreeding coefficients were 0.78, -0.15, and 0.17, and the Hardy-Weinberg probabilities were 0.70, 0.67, and 0.52. The genotypes evaluated did not have an effect on milk production; however, the single nucleotide polymorphisms can be used in studies on genetic variability.

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Section: Discussionmentioning

confidence: 99%

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

et al. 2016

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Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon

Silva

Filho

Silva

et al. 2021

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The objective this work was to evaluate genetically the buffaloes populations using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the leptin and stearoyl-CoA desaturase (SCD) genes, and associate the genotypes with milk production. Sixty-nine samples of Bubalus bubalis - 38 Murrah breed, 18 Mediterranean and 13 mixed-breed of the Murrah with Mediterranean - were evaluated. Allele and genotype frequencies, the heterozygosity observed and expected, the inbreeding coefficients (FIS), the probabilities of the Hardy-Weinberg equilibrium, the F statistic for population differentiation and Shannon index were calculated using GENEPOP and GenALEx programs. The associations of the different genotypes with a role in milk production were tested by Analysis of Variance (ANOVA) and t-test. We reported the leptin and SCD allele contribution to genetic variability in buffaloes herds in the Brazilian Amazonian region. The A allele was more representative in leptin and SCD genes for all breed groups. No significant effects between genotypes and milk production were found in the present study, but there is an indicative that AA genotype in leptin gene affects milk production in Murrah breed.

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Novel polymorphism in exon 1 of the melatonin receptor gene unassociated with reproductive characteristics of buffaloes in the Amazon Region

et al. 2016

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ABSTRACT. The objective of this study was to sequence part of the exon 1 in the melatonin receptor 1A gene (MTRN1A) in buffaloes to detect a novel polymorphism with which to associate reproductive characteristics, such as age at first birth and the interval between births, in buffaloes from the northeastern region of the State of Pará (Brazil). Buffalo hair samples (77) were collected from the Terra Firme region of Pará. DNA was extracted and polymerase chain reactions (PCRs) were carried out with a primer that was designed using the GenBank accession No. AY524665 reference sequence. PCR products were purified and sequenced. After editing and analysis of the sequences, a mutation was observed at the 62nd position in exon 1 of MTRN1A (T↔C), which corresponded with a change in the 21st amino acid from leucine to proline. All possible genotypes were observed, with the most common being genotype CC (0.481). The allele frequencies were T = 0.377 and C = 0.623. Statistical analysis of F IS showed inbreeding within the sample group (F IS = 0.397) and deviations from the HardyWeinberg equilibrium were observed (P < 0.05). Associations between genotypes and reproductive characteristics were not significant (P > 0.05). Although the related SNP was not synonymous, there were no observable effects on the reproductive characteristics under investigation. As such, it would be ideal to detect other SNPs in exon 1 of the MTRN1A gene that can be associated with reproductive characteristics in Amazonian buffaloes.

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Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon

Cited by 3 publications

References 14 publications

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon

Novel polymorphism in exon 1 of the melatonin receptor gene unassociated with reproductive characteristics of buffaloes in the Amazon Region

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